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Anti MOK protein kinase Polyclonal Antibody

Application

WB 0.05~0.2 µg/mL
IHC 0.5~2.0 µg/mL
ICC Not tested
ELISA Not tested
FCM Not tested
Neutralization Not tested
IP Not tested

<Western blotting>

Sample: COS7 cell extract
1. control
2. MOK protein kinase transfected

[Preparation of antibodies and instruction]:
Nishida E, Miyata Y
Department of Cell and Developmental Biology
Graduate School of Biostudies, Kyoto University

<Immunohistochemistry>

Sample: Mouse small intestine

[Preparation of antibodies and instruction]:
Uesaka T.
Laboratory for Neuronal Differentiation and Regeneration
Center for Developmental Biology, RIKEN

Character

Package Size 25µg (100µL / vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage Store below -20°C
Once thawed, store at 4°C Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was purified from rabbit serum immunized with recombinant mouse MOK containing C-terminal 111 amino acid by antigen affinity chromatography.

Product List

Product Name Cat# Quantity Price

Anti MOK

KAL-KR085 25UG

¥ 49,000
$ 654
€ 490

Citation
  • Regulation of cilium length and intraflagellar transport by the RCK-kinases ICK and MOK in renal epithelial cells.
    Broekhuis JR, Verhey KJ, Jansen G.
    PLoS One. 2014 Sep 22;9(9):e108470. PMID: 25243405

References
  • CK2 controls multiple protein kinases by phosphorylating a kinase-targeting molecular chaperone, Cdc37.
    Miyata Y, Nishida E.
    Mol Cell Biol. 2004 May;24(9):4065-74. PMID:
  • A novel MAP kinase regulates flagellar length in Chlamydomonas.
    Berman SA, Wilson NF, Haas NA, Lefebvre PA.
    Curr Biol. 2003 Jul 1;13(13):1145-9. PMID: 12842015
  • Specific association of a set of molecular chaperones including HSP90 and Cdc37 with MOK, a member of the mitogen-activated protein kinase superfamily.
    Miyata Y, Ikawa Y, Shibuya M, Nishida E.
    J Biol Chem. 2001 Jun 15;276(24):21841-8. PMID: 11278794
  • Molecular cloning and characterization of a novel member of the MAP kinase superfamily.
    Miyata Y, Akashi M, Nishida E.
    Genes Cells. 1999 May;4(5):299-309. PMID: 10421840
  • Distantly related cousins of MAP kinase: biochemical properties and possible physiological functions.
    Miyata Y, Nishida E.
    Biochem Biophys Res Commun. 1999 Dec 20;266(2):291-5. PMID: 10600495
  • A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma.
    Gaugler B, Brouwenstijn N, Vantomme V, Szikora JP, Van der Spek CW, Patard JJ, Boon T, Schrier P, Van den Eynde BJ.
    Immunogenetics. 1996;44(5):323-30. PMID: 8781117

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.