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For the study of glomerular epithelial cells

Anti Rat Podocalyxin Polyclonal Antibody

Background

Podocalyxin is the major sialoprotein of glomerular epithelial cells and contains most of the sialic acid in these cells. Podocalyxin aids in maintaining filtration slit patency and in the maintainance of foot process architecture. Podocalyxin is linked to ezrin and the actin cytoskeleton through interaction with the scaffold protein NHERF2 (Na+/H+-exchanger regulatory factor 2). Podocalyxin has been found in other tissues, though its function in these tissues is unclear.

Application

Western Blotting : 2~5μg / mL
Immunofluorescence : 10μg / mL

Western blotting

Sample:200μg/lane
(1) Rat liver lysate (Negative control)
(2) Rat kidney lysate
(3) Rat heart lysate

Preparation of antibodies and instruction:
Dr. Takeda T. and Dr. Gejyo F. at Department of Applied Molecular Medicine, Niigata University Graduate School of Medical and Dental Sciences

Character

Package Size 25μg (100μL/vial)
Format
Buffer
Rabbit polyclonal antibody 0.25mg/mL
PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage
Purification method
Below –20°C
This antibody was purified from rabbit serum immunized with synthesized partial peptide of Rat Podocalyxin by peptide affinity chromatography.

<Reference>
1. Takeda T. et. al., J Clin Invest 2001 Jul;108(2):289-301. PMID : 11457882
2. Kerjaschki D. et al: Am J Pathol 1985 Mar;118(3):343-9. PMID : 3976840
3. Kerjaschki D. et al.: J Cell Biol 1984;98:1591-6. PMID: 6371025

Product List

Product Name Cat# Quantity Price

Anti Podocalyxin

KAL-KR064 25UG

¥ 49,000
$ 654
€ 490