This antibody doesn't react to Calnexin, and recognizes only Calnexin Δ185-520.
Preparation of antibodies and instruction :
Department of Molecular Medicine,
Faculty of Medical and Pharmaceutical Sciences, Kumamoto University
1. Okiyoneda T, Wada I, Jono H, Shuto T, Yoshitake K, Nakano N, Nagayama S, Harada K, Isohama Y, Miyata T, Kai H. Calnexin Delta 185-520 partially reverses the misprocessing of the Delta F508 cystic fibrosis transmembrane conductance regulator. FEBS Lett. 2002 Aug 28;526(1-3):87-92.
2.Okiyoneda T, Kai H. Introduction of the conventional method of DNA transfection by adenovirus vector Nippon Yakurigaku Zasshi. 2002 Jun;119(6):337-44. Japanese.
|Format||Mouse Monoclonal Antibody 0.25mg/mL|
|Buffer||PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]|
|Storage||Store below -20°C
Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
|Purification method||The splenic lymphocytes from BALB/c mouse, immunized with partial peptide of Calnexin Δ185-520 were fused to myeloma P3U1 cells. The hybridoma is proliferated by serum free medium, and collects the conditioned medium, and IgG is refined with Protein G Affinity column.|
|Working dilution||For western blotting ;0.2〜1.0g/mL|