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useful for detection of normal peripheral blood T cells, some neoplastic T cells in human.

Anti CD3 Monoclonal Antibody (Clone No. NU-T3)

Background

The CD3-T cell receptor (TCR) complex plays a central role in the recognition of the antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. There are two types of TCR differentiated by their heterodimers, namely TCRαβ and TCRγδ. The CD3 antigen is comprised of multiple subunits (CD3γ, CD3δ, CD3ε, and CD3ζ). The variable immunoglobulin domains of TCRαβ (TCRγδ) bind to the ligand, whereas the cytoplasmic tails of the CD3 subunits interact with cytosolic-signalling proteins. This antibody is specific to human T cell associated-antigen and will be useful for detection of normal peripheral blood T cells, some neoplastic T cells in human.

Application

WB Not tested
IHC 0.5-1.0 μg/mL
ICC Not tested
ELISA Not tested
FCM 0.5-1.0 μg/mL
Neutralization Not tested
IP Not tested

Character

Primary Source -
Type Monoclonal
Immunogen Human peripheral blood T cell
Raised in Mouse
Myeloma NS-1
Clone number NU-T3
Isotype IgG2a
Source Serum-free medium
Purification notes ProteinG
Cross Reactivity Not yet tested in other species.
Concentration 0.25 mg/mL
Contents (Volume) 50 μg (200 μL/vial)
Label Unlabeled
Buffer PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]
Storage Store below -20 °C. Once thawed, store at 4 °C. Repeated freeze-thaw cycles should be avoided.

<Reference>
1. Call ME. et al.: Stoichiometry of the T-cell receptor-CD3 complex and key intermediates assembled in the endoplasmic reticulum. EMBO J. 2004 Jun 16;23(12):2348-57. Epub 2004 May 20.

2. Schamel WW. et al.: Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response. Exp Med. 2005 Aug 15;202(4):493-503. Epub 2005 Aug 8.

3. Ma MC. et al.: Oxygen-sensitive {delta}-opioid receptor-regulated survival and death signals: novel insights into neuronal preconditioning and protection. J Biol Chem. 2005 Apr 22;280(16):16208-18. Epub 2005 Feb 1.

Product List

Product Name Cat# Quantity Price

Anti CD3

KAL-KN141 50UG

¥ 55,000
$ 734
€ 550

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.