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Antibody to elucidate the nocifensive response to pain in vivo, and the molecular mechanism for sensitization-desensitization

Anti Rat phospho TRPV1 Polyclonal Antibody and TRP rerlated products

For Research Use Only

Background

Recent work has begun to clarify the function of ionotropic receptors, which are expressed in sensory neurons and promote nociception. The most representative receptors of this class belong to TRP ion channel superfamily comprised by seven sub-families; TRPC, TRPV, TRPP, TRPM, TRPN, TRPML and TRPA. TRPVl channels have six transmembrane domains that most probably assemble into tetramers to form non-selective cationic channels. The first cloned TRPV receptor was TRPV1. This receptor is activated by capsaicin, protons or heat (with a threshold > ~43°C), all of which cause pain in vivo. The recent researches on nociception and stimulus conduction systems have focused on TRPV1. Such research indicates that TRPV1 activity is regulated by protein phosphorylation and dephosphorylation, and plays key roles in the mechanism of acute inflammatory nociception.
TRPV1 activity is enhanced by PKC activity induced by the inflammatory mediators adenosine triphosphate (ATP), bradykinin(BK), prostaglandins(PGx) through G protein-coupled receptors.
TRPV1 has two phosphorylation sites for PKC-mediated phosphorylation: S502 and S800(Ref. 1).
The antibody presented here reacts with phosphorylated TRPV1 at S800. PKCe was identified as the target of S800 phosphorylation and there is evidence to support the in vivo phosphoryation of S800 in mouse and rat DRG neurons by PKCe(Ref. 2). This antibody will be useful to elucidate the nocifensive response to pain in vivo, and the molecular mechanism for sensitization-desensitization.

Package Size 25μg(100μL/vial)
Format Rabbit polyclonal antibody 0.25mg/mL
Buffer PBS [containing 50% Block Ace as a stabilizer, 0.1%Proclin as a bacteroopstat]
Storage Store below 20°C
  Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.
Purification method This antibody was established from the serum of a rabbit immunized with the partial peptide representing phosphorylated TRPV1 at S800, and purified by peptide affinity chromatography.
Working dilution For Western blotting:0.5μg/ml

Western blotting
Sample: phosphorylated TRPV1 at S800 in HEK293 cells

Preparation of antibodies and instruction
Dr. Makoto Tominaga at Section of Cell Signaling,
Okazaki Institute for Integrative Bioscience,
National Institutes of Natural Sciences

 

References

1. Tominaga M. et al. : Thermosensation and pain. J Neurobiol. 2004 Oct;61(1):3-12. Review.
2. Mandadi S. et al. : Increased sensitivity of desensitized TRPV1 by PMA occurs through PKCepsilon-mediated phosphorylation at S800.
Pain. 2006 Jul; 123(1-2): 106-16.*
* : Application Reference

Product List

Product Name Cat# Quantity Price

Anti TRPV1 / VR-1, phospho Ser800

KAL-KM112 25UG

¥ 49,000
$ 654
€ 490

Anti TRPV1 / VR-1

KAL-KM018 5UG

¥ 49,000
$ 654
€ 490

Anti TRPV2 / VRL-1

KAL-KM019 5UG

¥ 49,000
$ 654
€ 490

Anti TRPV 4

KAL-KM119 25UG

¥ 49,000
$ 654
€ 490

Anti TRPA 1

KAL-KM120 50UG

¥ 49,000
$ 654
€ 490

Anti Rim1

KAL-KO454 25UG

¥ 49,000
$ 654
€ 490

Anti Cacnb4

KAL-KO455 25UG

¥ 49,000
$ 654
€ 490

Anti Trpc3

KAL-KO456 25UG

¥ 49,000
$ 654
€ 490

Anti Trpc3

KAL-KO457 25UG

¥ 49,000
$ 654
€ 490

Anti Trpc5

KAL-KO458 25UG

¥ 49,000
$ 654
€ 490

Anti Trpc5

KAL-KO459 25UG

¥ 49,000
$ 654
€ 490

Anti Trpc6

KAL-KO460 25UG

¥ 49,000
$ 654
€ 490

Anti Trpm2

KAL-KO462 25UG

¥ 49,000
$ 654
€ 490

Anti Trpm2

KAL-KO463 25UG

¥ 49,000
$ 654
€ 490

Anti Trpm7

KAL-KO464 25UG

¥ 49,000
$ 654
€ 490

1. Tominaga M. et al. : Thermosensation and pain. J Neurobiol. 2004 Oct;61(1):3-12. Review. 2. Mandadi S. et al. : Increased sensitivity of desensitized TRPV1 by PMA occurs through PKCepsilon-mediated phosphorylation at S800. Pain. 2006 Jul; 123(1-2): 106