Polyamines are generally believed to function both in protein synthesis and DNA synthesis leading to control cell proliferation. In 1971, Russel firstly reported that total amount of urinary polyamines elevated in cancer patients. And quantitative kit of urinary polyamines were already developed and utilized as a general biochemical examination.
Recently two diacetyl-derivatives, N1,N12-diacetylspermine and N1,N8-diacetylspermidine, were found to be excreted in urine and form 0.6% and 1.4% of total polyamines respectively.
Comparing urine of diseased person with urine of healthy person, some reports suggested the possibility that diacetyl-derivatives correlate to the status of disease more closely than total amount of polyamines.
Our kit is convenient to quantify amount of urinary diacetylspermine by using ELISA method. This kit is only for research use, not for diagnosis.
Highly sensitive and specific
Strip type well, antigen pre-coated microplate
Assay range: 6.25~200nM
|(1)||Antigen coated microtiter plate,96 wells||1 plate|
|(2)||Diacetylspermine standard||250l × 2|
|(3)||Antibody diluent||20mL × 1|
|(4)||Anti Diacetylspermine antibody concentrate(×100)||80l × 1|
|(5)||HRP-anti Rabbit IgG Antibody concentrate(×80)||60l × 1|
|(6)||OPD (o-phenylendiamine) tablets||2 tab.|
|(7)||Substrate solution||30 mL × 1|
|(8)||Stop solution||15 mL × 1|
|(9)||Wash buffer concentrate (×20)||30 mL × 1|
|(10)||Dilution plate||1 plate|
Diacetylspermine (Urinary) ELISA