Magnosphere MS300/Streptavidin particles are Streptavidin coated magnetic microparticles for bioseparation. The surfaces of Magnosphere MS300/Streptavidin particles are covered with hydrophilic polymer to achieve both low non-specific binding and no inhibition of enzyme activity. Consequently, Magnosphere MS300/Streptavidin particles can be used for PCR amplification systems or enzyme immunoassay systems.
PCR, quantitative PCR
Capturing biotinylated proteins
Example of use 1 Influence on PCR
Added amount of Magnosphere to the PCR cocktail (mg/tube)
Various amount of Magnosphere MS300/Streptavidin were added to PCR cocktail with defined amount of 100bp DNA and the DNA were amplified by qPCR. Obtained Threshold Cycle (Ct) were plotted against added bead amount. As a result, Ct values were not affected by addition of Magnosphere MS300/Streptavidin through 3mg/tube.
Example of use 2
Added amount of biotin labeled DNA (copies)
Added vs. immobilized DNA on the Magnosphere MS300/Streptavidin. According to the protocol above, various amount of biotin labeled 100bp DNA were reacted with the Magnosphere MS300/Streptavidin. The amount of immobilized DNA were quantitated through a qPCR using bead fraction.
This product is for research use only and not intended for the other purposes such as therapeutic or diagnostic use. Refer to or verify all regulatory and safety information, and determine suitability of this product before use. The specifications of the product are subjected to change without notice.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.