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This is a tool for efficient introduction of IgG antibodies into living cells using HVJ-E envelope (HVJ-E) vector for the purpose of analysis of the functions of cells and intracellular proteins.

GenomONE™ - CAb EX Antibody Delivery Reagent-Performance compared with existing reagents for protein introduction

Performance compared with existing reagents for protein introduction

1.Hs68 cells(anti-NPC antibody)
 /observed by confocal laser scanning microscopy

【Cells】
Hs68: Human, foreskin fibroblast (ATCC-CRL-1635), 5×103 cells/well

【Antibody delivered into cells】
Monoclonal Anti-βActin, Clone AC-15, Mouse IgG1(SIGMA, A5441)

2. Hs68, HeLa S3A549 cells(anti-NPC antibody) /observed by confocal laser scanning microscopy

【Cells】
Hs68: Human, foreskin fibroblast (ATCC CRL-1635), 5×103 cells/well
HeLa S3: Human, cervical epithelioid carcinoma (ATCC CCL 2.2), 2×104 cells/well
A549: Human, lung carcinoma (ATCC CCL-185), 2×104 cells/well

【Antibody】
Monoclonal Anti-Nuclear Pore Complex Proteins, Clone 414, Mouse IgG1 (SIGMA, N8786)