HVJ (hemagglutinating virus of Japan) Envelope VECTOR KIT is a tool for transfection of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion.
The HVJ envelope, carrying the molecule to be transfected, is composed of a completely inactivated and purified HVJ (Sendai virus) while preserving the cell membrane-fusing capability of the envelope.
GenomONE provides ready-to-use kits containing the HVJ envelope and auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, buffer).
[Transfection, Cell fusion] Flyer
What is HVJ Envelope (HVJ-E)?
HVJ Envelope is a purified product prepared through complete inactivation of Sendai virus (HVJ*). It is a vesicle in which only the cell membrane-fusing capability of the envelope protein of Sendai virus is retained.
It is known that the HN protein in the tunica externa of the Sendai virus recognizes the receptor (possessing sialic acid at the terminal of sugar chain) on the cell membrane and adsorbs it, leading to the induction of membrane fusion mediated by F protein (another component of the envelope). The genomic RNA of the Sendai virus contained in HVJ-E has been inactivated completely and has neither infective nor proliferative potentials in humans or experimental animals. HVJ-E can be used safely at ordinary laboratories, without requiring any special operations or facilities.
*HVJ:Hemagglutinating Virus of Japan.
Conventional non-viral transfection tools, including cationic lipids, are incorporated into cells through endocytosis which results in degradation of the transferred DNA by lysosomes.
On the other hand, HVJ Envelope VECTOR resists degradation by lysosomes, making it easy to transfer the specified DNA. Therefore, HVJ Envelope VECTOR yields highly efficient gene expression.
Sialic acid receptors, which are needed to trigger binding with HN protein, exist in almost all animal cells. Thus, HVJ Envelope VECTOR is useful for a wide range of targets.
1. Wide usability
GenomONE HVJ Envelope VECTOR KIT is a highly flexible tool for transfecting a wide variety of molecules (plasmid DNAs, siRNAs, oligonucleotides, proteins, antibodies etc.) into cultured cells (adherent and non-adherent) and tissue. GenomONE is useful for transfecting sensitive primary cells and is further distinguished by its ability to deliver contents into live animals. Many literature citations are available for each GenomONE-Neo application.
Unlike cationic lipids, GenomONE-Neo delivers the molecule of interest into cells via membrane fusion, not by endocytosis where cargo may be degraded by lysosomal enzymes. Since GenomeONE-Neo is a purified HVJ (Sendai virus) product, prepared from virus particles completely inactivated for infectious ability and proliferative potential it is completely safe to use without special precautions.
GenomONE provides ready-to-use kits containing the HVJ envelope and required auxiliary reagents (incorporation enhancer, incorporation reagent, introduction enhancer, and buffer). Suggested protocols for all major applications are included.
1. Comparison of GenomONE-Neo with cationic lipid-based reagent for siRNA delivery
1. Phospholamban (PLB) gene knockdown in primary rat myocardial cell cultures
|Cationic lipid reagent
1: PLB siRNA (10µg )
〈Data〉 Drs. M. Arai and A. Watanabe (Gunma University Graduate School of Medicine)
〈Related article〉 Watanabe et al., J. Mol. Cell. Cardiol., 37 (3), 691-698 (2004)
2 Bin1 gene knockdown in C2C12 (mouse myoblast cell line) after induction of differentiation
〈Data〉 Drs. C. Kojima* and H. Sabe (Osaka Bioscience Institute, *Current address: Osaka Prefecture University)
〈Related article〉 Kojima et al., EMBO J., 23, 4413-4422 (2004).
3 Eg5 gene knockdown in various cell cultures
4 EGFP gene knockdown in monkey ES cells
Biochem. Biophys. Res. Commun., 331, 1039-1044 (2005).
2. Highly efficient in vivo gene transfer to the mouse uterus
-Comparison data with cationic lipid-based reagent-
Mol. Hum. Reprod., 9, 603-609 (2003)
3.Transfection of neuroprogenitor cells with iron nanoparticles for magnetic resonance imaging tracking
-Comparison data with cationic lipid-based reagent-
Mol. Imaging Biol., 7, 286-295 (2005).
TOPICS (Representative Application Examples)
Applications for difficult-to-transfect cells and primary cells as well as in vivo experiments.
GenomONE-NEO EX siRNA-data
GenomONE-NEO EX Protein-data
 siRNA transfectionin vitro
 Protein deliveryin vitro
 Oligo DNA transfectionin vitro
 Plasmid DNA transfectionin vivo
GenomONE - Neo EX Instruction Manual
|GenomONE-Neo EX Kit Contents||GN001EX||GN004EX|
|0.26 ml||1 tube||4 tubes|
|reagent A||0.5 ml||1 tube||1 tube|
|reagent B||0.3 ml||1 tube||1 tube|
|reagent C||1.0 ml||1 tube||1 tube|
|Buffer||6.5ml||1 tube||1 tube|
The HVJ Envelope VECTOR KIT "GenomONE-Neo EX" series consists of HVJ Envelope and sub reagent set. Sub reagent set consists of 3 reagents (reagent A, reagent B and reagent C) and buffer for suspension or dilution.
GenomONE-Neo EX HVJ-E 1 vial Transfection Reagents
GenomONE-Neo EX HVJ-E 4 vials Transfection Reagents
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.