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Superior to PEG for hybridoma preparation and other cell fusion procedures

GenomONE™ - CF EX Sendai virus (HVJ) Envelope Cell Fusion Kit - References

Cell Fusion

Nuclear transfer
Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes.
Yamada M, Egli D.
Stem Cell Reports. 2017 Mar 14;8(3):576-588. PMID: 28242217
Analysis of cellular function
Developmentally Regulated RNA-binding Protein 1 (Drb1)/RNA-binding Motif Protein 45 (RBM45), a Nuclear-Cytoplasmic Trafficking Protein, Forms TAR DNA-binding Protein 43 (TDP-43)-mediated Cytoplasmic Aggregates.
Mashiko T, Sakashita E, Kasashima K, Tominaga K, Kuroiwa K, Nozaki Y, Matsuura T, Hamamoto T, Endo H.
J Biol Chem. 2016 Jul 15;291(29):14996-5007. PMID: 27226551
Analysis of cellular function
Color-coded Live Imaging of Heterokaryon Formation and Nuclear Fusion of Hybridizing Cancer Cells.
Suetsugu A, Matsumoto T, Hasegawa K, Nakamura M, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
Anticancer Res. 2016 Aug;36(8):3827-31. PMID: 27466484
Chromosome Transfer
Quantitative assessment of chromosome instability induced through chemical disruption of mitotic progression.
Markossian S, Arnaoutov A, Saba NS, Larionov V, Dasso M.
Cell Cycle. 2016 Jul 2;15(13):1706-14. PMID: 27104376
Pronuclear Transplantation
Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease.
Hyslop LA1,2, Blakeley P3, Craven L4, Richardson J1, Fogarty NM3, Fragouli E5, Lamb M, Wamaitha SE, Prathalingam N, Zhang Q, O'Keefe H, Takeda Y, Arizzi L, Alfarawati S, Tuppen HA, Irving L, Kalleas D, Choudhary M, Wells D, Murdoch AP, Turnbull DM, Niakan KK, Herbert M.
Nature. 2016 Jun 16;534(7607):383-6. PMID: 27281217
Analysis of cellular function
Targeted and ultrasound-triggered cancer cell injury using perfluorocarbon emulsion-loaded liposomes endowed with cancer cell-targeting and fusogenic capabilities.
Ninomiya K, Yamashita T, Tanabe Y, Imai M, Takahashi K, Shimizu N.
Ultrason Sonochem. 2016 Jan;28:54-61. PMID: 26384883
Hybridoma production
Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin.
Honma R, Fujii Y, Ogasawara S, Oki H, Liu X, Nakamura T, Kaneko MK, Takagi M, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2016 Feb;35(1):41-7. PMID: 26788987
Hybridoma production
Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis.
Honma R, Fujii Y, Ogasawara S, Oki H, Konnai S, Kagawa Y, Takagi M, Kaneko MK, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2016 Apr;35(2):65-72. PMID: 26977704
Mitochondria transfer
Selective elimination of mitochondrial mutations in the germline by genome editing.
Reddy P, Ocampo A, Suzuki K, Luo J, Bacman SR, Williams SL, Sugawara A, Okamura D, Tsunekawa Y, Wu J, Lam D, Xiong X, Montserrat N, Esteban CR, Liu GH, Sancho-Martinez I, Manau D, Civico S, Cardellach F, Del Mar O'Callaghan M, Campistol J, Zhao H, Campistol JM, Moraes CT, Izpisua Belmonte JC.
Cell. 2015 Apr 23;161(3):459-69. PMID: 25910206
Hybridoma production
Characterization of monoclonal antibody LpMab-3 recognizing sialylated glycopeptide of podoplanin.
Oki H, Ogasawara S, Kaneko MK, Takagi M, Yamauchi M, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2015 Feb;34(1):44-50. PMID: 25723283
Chromosome Transfer
Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTAVP64 expression cassette.
Kononenko AV, Lee NC, Liskovykh M, Masumoto H, Earnshaw WC, Larionov V, Kouprina N.
Nucleic Acids Res. 2015 May 19;43(9):e57. PMID: 25712097
Analysis of cellular function
New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique.
Takahashi Y, Tomotsune D, Takizawa S, Yue F, Nagai M, Yokoyama T, Hirashima K, Sasaki K.
World J Stem Cells. 2015 Jun 26;7(5):883-93. PMID: 26131319
Hybridoma production
Human T-cell leukemia virus type-I Tax induces the expression of CD83 on T cells.
Tanaka Y, Mizuguchi M, Takahashi Y, Fujii H, Tanaka R, Fukushima T, Tomoyose T, Ansari AA, Nakamura M.
Retrovirology. 2015 Jul 1;12:56. PMID: 26129803
Analysis of cellular function
Cardiac Stem Cell Hybrids Enhance Myocardial Repair.
Quijada P, Salunga HT, Hariharan N, Cubillo JD, El-Sayed FG, Moshref M, Bala KM, Emathinger JM, De La Torre A, Ormachea L, Alvarez R Jr, Gude NA, Sussman MA.
Circ Res. 2015 Sep 25;117(8):695-706. PMID: 26228030
Hybridoma production
Tau monoclonal antibody generation based on humanized yeast models: impact on Tau oligomerization and diagnostics.
Rosseels J, Van den Brande J, Violet M, Jacobs D, Grognet P, Lopez J, Huvent I, Caldara M, Swinnen E, Papegaey A, Caillierez R, Buee-Scherrer V, Engelborghs S, Lippens G, Colin M, Buee L, Galas MC, Vanmechelen E, Winderickx J.
J Biol Chem. 2015 Feb 13;290(7):4059-74. PMID: 25540200
Chromosome Transfer
Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice.
Liskovykh M, Ponomartsev S, Popova E, Bader M, Kouprina N, Larionov V, Alenina N, Tomilin A.
Cell Cycle. 2015;14(8):1268-73. PMID: 25695642
Hybridoma production
Monoclonal Antibody LpMab-9 Recognizes O-glycosylated N-Terminus of Human Podoplanin.
Kaneko MK, Oki H, Hozumi Y, Liu X, Ogasawara S, Takagi M, Goto K, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2015 Oct;34(5):310-7. PMID: 26492618
Analysis of cellular function
Developmental potential of 2n/3n mixoploid mouse embryos produced by fusion of individual second polar bodies and blastomeres of 2-cell embryos.
Hino T, Tateno H.
Reprod Fertil Dev. 2015 Jul 8. PMID: 26151553
Nuclear transfer
Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos.
Kang E, Wu G, Ma H, Li Y, Tippner-Hedges R, Tachibana M, Sparman M, Wolf DP, Scholer HR, Mitalipov S.
Nature. 2014 May 1;509(7498):101-4. PMID: 24670652
Nuclear transfer
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada M, Johannesson B, Sagi I, Burnett LC, Kort DH, Prosser RW, Paull D, Nestor MW, Freeby M, Greenberg E, Goland RS, Leibel RL, Solomon SL, Benvenisty N, Sauer MV, Egli D.
Nature. 2014 Jun 26;510(7506):533-6. PMID: 24776804
Nuclear transfer
Polar body genome transfer for preventing the transmission of inherited mitochondrial diseases.
Wang T, Sha H, Ji D, Zhang HL, Chen D, Cao Y, Zhu J.
Cell. 2014 Jun 19;157(7):1591-604. PMID: 24949971
Analysis of cellular function
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
Saka SK, Honigmann A, Eggeling C, Hell SW, Lang T, Rizzoli SO5.
Nat Commun. 2014 Jul 25;5:4509. PMID: 25060237
Hybridoma production
A cancer-specific monoclonal antibody recognizes the aberrantly glycosylated podoplanin.
Kato Y, Kaneko MK.
Sci Rep. 2014 Aug 1;4:5924. PMID: 25080943
Chromosome Transfer
A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function.
Kononenko AV, Bansal R, Lee NC, Grimes BR, Masumoto H, Earnshaw WC, Larionov V, Kouprina N.
Nucleic Acids Res. 2014 Dec 1;42(21). PMID: 25260588
Nuclear transfer
Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.
Neupane J, Vandewoestyne M, Ghimire S, Lu Y, Qian C, Van Coster R, Gerris J, Deroo T, Deforce D, De Sutter P, Heindryckx B.
Mitochondrion. 2014 Sep;18:27-33. PMID: 25229667
Mitochondria transfer
Cell fusion through a microslit between adhered cells and observation of their nuclear behavior.
Wada K, Hosokawa K, Kondo E, Ito Y, Maeda M.
Biotechnol Bioeng. 2014 Jul;111(7):1464-8. PMID: 24420735
Nuclear transfer
Embryonic development following somatic cell nuclear transfer impeded by persisting histone methylation.
Matoba S, Liu Y, Lu F, Iwabuchi KA, Shen L, Inoue A, Zhang Y.
Cell. 2014 Nov 6;159(4):884-95. PMID: 25417163
Analysis of cellular function
A biotin-streptavidin-biotin bridge dramatically enhances cell fusion.
Li J, Yu X, Wagner TE, Wei Y.
Oncol Lett. 2014 Jul;8(1):198-202. PMID: 24959245
Analysis of cellular function
Transitory FGF treatment results in the long-lasting suppression of the proliferative response to repeated FGF stimulation.
Poole A, Knowland N, Cooper E, Cole R, Wang H, Booth L, Kacer D, Tarantini F, Friesel R, Prudovsky I.
J Cell Biochem. 2014 May;115(5):874-88. PMID: 24375433
Nuclear transfer
Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants.
Paull D, Emmanuele V, Weiss KA, Treff N, Stewart L, Hua H, Zimmer M, Kahler DJ, Goland RS, Noggle SA, Prosser R, Hirano M, Sauer MV, Egli D.
Nature. 2013 Jan 31;493(7434):632-7. PMID: 23254936
Chromosome Transfer
Towards germline gene therapy of inherited mitochondrial diseases.
Tachibana M, Amato P, Sparman M, Woodward J, Sanchis DM, Ma H, Gutierrez NM, Tippner-Hedges R, Kang E, Lee HS, Ramsey C, Masterson K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer R, Mitalipov S.
Nature. 2013 Jan 31;493(7434):627-31. PMID: 23103867
Pronuclear Transplantation
Production of offspring after sperm chromosome screening: an experiment using the mouse model.
Watanabe H, Kusakabe H, Mori H, Yanagimachi R, Tateno H.
Hum Reprod. 2013 Feb;28(2):531-7. PMID: 23136143
Mitochondria transfer
Chromosome transfer in mature oocytes.
Tachibana M, Sparman M, Mitalipov S.
Nat Protoc. 2010 Jun;5(6):1138-47. PMID: 20539289
Pronuclear Transplantation
Pronuclear transfer in human embryos to prevent transmission of mitochondrial DNA disease.
Craven L, Tuppen HA, Greggains GD, Harbottle SJ, Murphy JL, Cree LM, Murdoch AP, Chinnery PF, Taylor RW, Lightowlers RN, Herbert M, Turnbull DM.
Nature. 2010 May 6;465(7294):82-5. PMID: 20393463
Mitochondrial gene replacement
Mitochondrial gene replacement in primate offspring and embryonic stem cells.
Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O, Mitalipov S.
Nature. 2009 Sep 17;461(7262):367-72. PMID: 19710649

Product List

Product Name Cat# Quantity Price

GenomONE-CF EX SeV-E (HVJ-E) 1 vial Cell Fusion Reagents

ISK-CF-001-EX 1SET

¥ 25,000
$ 334
€ 250

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.