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Superior to PEG for hybridoma preparation and other cell fusion procedures

GenomONE™ - CF EX Sendai virus (HVJ) Envelope Cell Fusion Kit - References

Cell Fusion

                                                     
Analysis of cellular function
Relationship between OPA1 and cardiolipin in mitochondrial inner-membrane fusion.
T. Ban et al.
Biochim. Biophys. Acta.,1859(9) 951-957 (2018). PMID: 29852142
Somatic Cell Nuclear Transfer
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer.
R. Azuma et al.
J. Vis. Exp., 134 (2018). PMID: 29757287
Nuclear transfer
Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer
Zhen Liu, Yijun Cai, Yan Wang, Yanhong Nie, Chenchen Zhang, Yuting Xu, Xiaotong Zhang, Yong Lu, Zhanyang Wang, Muming Poo, Qiang Sun
Cell. 2018 Jan 20. pii: S0092-8674(18)30057-6 PMID: 29395327
Nuclear transfer
Genomic instability during reprogramming by nuclear transfer is DNA replication dependent.
G. Chia et al.
Nature Cell Biology 19, 282–291 (2017) PMID: 28263958
Nuclear transfer
Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes.
Yamada M, Egli D.
Stem Cell Reports. 2017 Mar 14;8(3):576-588. PMID: 28242217
Nuclear transfer
Correction of β-thalassemia mutant by base editor in human embryos.
Liang P, Ding C, Sun H, Xie X, Xu Y, Zhang X, Sun Y, Xiong Y, Ma W, Liu Y, Wang Y, Fang J, Liu D, Songyang Z, Zhou C, Huang J
Protein Cell. 2017 Nov;8(11):811-822 PMID: 28942539
mitochondria transfer
Quantitative control of mitochondria transfer between live single cells using a microfluidic device.
K. Wada et al.
Biol. Open, 6(12) 1960-1965 (2017). PMID: 29092814
Preparation of monoclonal antibodies
Establishment of CMab-43, a Sensitive and Specific Anti-CD133 Monoclonal Antibody, for Immunohistochemistry.
S. Itai et al.
Monoclon. Antib. Immunodiagn. Immunother., 36(5), 231-235 (2017). PMID: 28910211
Preparation of monoclonal antibodies
Development of EMab-51, a Sensitive and Specific Anti-Epidermal Growth Factor Receptor Monoclonal Antibody in Flow Cytometry, Western Blot, and Immunohistochemistry.
S. Itai et al.
Monoclon. Antib. Immunodiagn Immunother., 36(5), 214-219 (2017). PMID: 28891752
Preparation of monoclonal antibodies
H2Mab-77 is a Sensitive and Specific Anti-HER2 Monoclonal Antibody Against Breast Cancer.
S. Itai et al.
Monoclon Antib Immunodiagn Immunother. 36(4), 143-148(2017). PMID: 28700270
Nuclear transfer
Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules.
Miyamoto K, Tajima Y, Yoshida K, Oikawa M, Azuma R, Allen GE, Tsujikawa T, Tsukaguchi T, Bradshaw CR, Jullien J, Yamagata K, Matsumoto K, Anzai M, Imai H, Gurdon JB, Yamada M.
Biol Open. 2017 Apr 15;6(4):415-424. PMID: 28412714
Analysis of cellular function
Color-coded Live Imaging of Heterokaryon Formation and Nuclear Fusion of Hybridizing Cancer Cells.
Suetsugu A, Matsumoto T, Hasegawa K, Nakamura M, Kunisada T, Shimizu M, Saji S, Moriwaki H, Bouvet M, Hoffman RM.
Anticancer Res. 2016 Aug;36(8):3827-31. PMID: 27466484
Analysis of cellular function
Developmentally Regulated RNA-binding Protein 1 (Drb1)/RNA-binding Motif Protein 45 (RBM45), a Nuclear-Cytoplasmic Trafficking Protein, Forms TAR DNA-binding Protein 43 (TDP-43)-mediated Cytoplasmic Aggregates.
Mashiko T, Sakashita E, Kasashima K, Tominaga K, Kuroiwa K, Nozaki Y, Matsuura T, Hamamoto T, Endo H.
J Biol Chem. 2016 Jul 15;291(29):14996-5007. PMID: 27226551
Chromosome Transfer
Quantitative assessment of chromosome instability induced through chemical disruption of mitotic progression.
Markossian S, Arnaoutov A, Saba NS, Larionov V, Dasso M.
Cell Cycle. 2016 Jul 2;15(13):1706-14. PMID: 27104376
Preparation of monoclonal antibodies
Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis.
R. Honma et al.
Monoclon. Antib. Immunodiagn. Immunother.,35(2) 65-72 (2016). PMID: 26977704
Analysis of cellular function
Post-Testicular Sperm Maturation: Centriole Pairs, Found in Upper Epididymis, are Destroyed Prior to Sperm's Release at Ejaculation.
Simerly C, Castro C, Hartnett C, Lin CC, Sukhwani M, Orwig K, Schatten G.
Sci Rep. 2016 Aug 18;6:31816. PMID: 27534805
Pronuclear Transplantation
Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease.
Hyslop LA1,2, Blakeley P3, Craven L4, Richardson J1, Fogarty NM3, Fragouli E5, Lamb M, Wamaitha SE, Prathalingam N, Zhang Q, O'Keefe H, Takeda Y, Arizzi L, Alfarawati S, Tuppen HA, Irving L, Kalleas D, Choudhary M, Wells D, Murdoch AP, Turnbull DM, Niakan KK, Herbert M.
Nature. 2016 Jun 16;534(7607):383-6. PMID: 27281217
Analysis of cellular function
Targeted and ultrasound-triggered cancer cell injury using perfluorocarbon emulsion-loaded liposomes endowed with cancer cell-targeting and fusogenic capabilities.
Ninomiya K, Yamashita T, Tanabe Y, Imai M, Takahashi K, Shimizu N.
Ultrason Sonochem. 2016 Jan;28:54-61. PMID: 26384883
Hybridoma production
Establishment of Novel Monoclonal Antibody PMab-32 Against Rabbit Podoplanin.
Honma R, Fujii Y, Ogasawara S, Oki H, Liu X, Nakamura T, Kaneko MK, Takagi M, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2016 Feb;35(1):41-7. PMID: 26788987
Hybridoma production
Critical Epitope of Anti-Rabbit Podoplanin Monoclonal Antibodies for Immunohistochemical Analysis.
Honma R, Fujii Y, Ogasawara S, Oki H, Konnai S, Kagawa Y, Takagi M, Kaneko MK, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2016 Apr;35(2):65-72. PMID: 26977704
Mitochondria transfer
Selective elimination of mitochondrial mutations in the germline by genome editing.
Reddy P, Ocampo A, Suzuki K, Luo J, Bacman SR, Williams SL, Sugawara A, Okamura D, Tsunekawa Y, Wu J, Lam D, Xiong X, Montserrat N, Esteban CR, Liu GH, Sancho-Martinez I, Manau D, Civico S, Cardellach F, Del Mar O'Callaghan M, Campistol J, Zhao H, Campistol JM, Moraes CT, Izpisua Belmonte JC.
Cell. 2015 Apr 23;161(3):459-69. PMID: 25910206
Hybridoma production
Characterization of monoclonal antibody LpMab-3 recognizing sialylated glycopeptide of podoplanin.
Oki H, Ogasawara S, Kaneko MK, Takagi M, Yamauchi M, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2015 Feb;34(1):44-50. PMID: 25723283
Chromosome Transfer
Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTAVP64 expression cassette.
Kononenko AV, Lee NC, Liskovykh M, Masumoto H, Earnshaw WC, Larionov V, Kouprina N.
Nucleic Acids Res. 2015 May 19;43(9):e57. PMID: 25712097
Analysis of cellular function
New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique.
Takahashi Y, Tomotsune D, Takizawa S, Yue F, Nagai M, Yokoyama T, Hirashima K, Sasaki K.
World J Stem Cells. 2015 Jun 26;7(5):883-93. PMID: 26131319
Hybridoma production
Human T-cell leukemia virus type-I Tax induces the expression of CD83 on T cells.
Tanaka Y, Mizuguchi M, Takahashi Y, Fujii H, Tanaka R, Fukushima T, Tomoyose T, Ansari AA, Nakamura M.
Retrovirology. 2015 Jul 1;12:56. PMID: 26129803
Analysis of cellular function
Cardiac Stem Cell Hybrids Enhance Myocardial Repair.
Quijada P, Salunga HT, Hariharan N, Cubillo JD, El-Sayed FG, Moshref M, Bala KM, Emathinger JM, De La Torre A, Ormachea L, Alvarez R Jr, Gude NA, Sussman MA.
Circ Res. 2015 Sep 25;117(8):695-706. PMID: 26228030
Hybridoma production
Tau monoclonal antibody generation based on humanized yeast models: impact on Tau oligomerization and diagnostics.
Rosseels J, Van den Brande J, Violet M, Jacobs D, Grognet P, Lopez J, Huvent I, Caldara M, Swinnen E, Papegaey A, Caillierez R, Buee-Scherrer V, Engelborghs S, Lippens G, Colin M, Buee L, Galas MC, Vanmechelen E, Winderickx J.
J Biol Chem. 2015 Feb 13;290(7):4059-74. PMID: 25540200
Chromosome Transfer
Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice.
Liskovykh M, Ponomartsev S, Popova E, Bader M, Kouprina N, Larionov V, Alenina N, Tomilin A.
Cell Cycle. 2015;14(8):1268-73. PMID: 25695642
Hybridoma production
Monoclonal Antibody LpMab-9 Recognizes O-glycosylated N-Terminus of Human Podoplanin.
Kaneko MK, Oki H, Hozumi Y, Liu X, Ogasawara S, Takagi M, Goto K, Kato Y.
Monoclon Antib Immunodiagn Immunother. 2015 Oct;34(5):310-7. PMID: 26492618
Analysis of cellular function
Developmental potential of 2n/3n mixoploid mouse embryos produced by fusion of individual second polar bodies and blastomeres of 2-cell embryos.
Hino T, Tateno H.
Reprod Fertil Dev. 2015 Jul 8. PMID: 26151553
Preparation of monoclonal antibodies
Monoclonal Antibody LpMab-9 Recognizes O-glycosylated N-Terminus of Human Podoplanin.
M k Kaneko et al.
Monoclon. Antib. Immunodiagn. Immunother.,34(5) 310-317 (2015). PMID: 26492618
Microcell-mediated chromosome transfer
Stable maintenance of de novo assembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice.
M. Liskovykh et al.
Cell Cycle, 14(8), 1268-1273 (2015). PMID: 25695642
Preparation of monoclonal antibodies
Tau monoclonal antibody generation based on humanized yeast models: impact on Tau oligomerization and diagnostics.
J. Rosseels et al.
J.Biol.Chem., 290(7) 4059-4074 (2015). PMID: 25540200
Nuclear transfer
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada M, Johannesson B, Sagi I, Burnett LC, Kort DH, Prosser RW, Paull D, Nestor MW, Freeby M, Greenberg E, Goland RS, Leibel RL, Solomon SL, Benvenisty N, Sauer MV, Egli D.
Nature. 2014 Jun 26;510(7506):533-6. PMID: 24776804
Nuclear transfer
Polar body genome transfer for preventing the transmission of inherited mitochondrial diseases.
Wang T, Sha H, Ji D, Zhang HL, Chen D, Cao Y, Zhu J.
Cell. 2014 Jun 19;157(7):1591-604. PMID: 24949971
Analysis of cellular function
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
Saka SK, Honigmann A, Eggeling C, Hell SW, Lang T, Rizzoli SO5.
Nat Commun. 2014 Jul 25;5:4509. PMID: 25060237
Hybridoma production
A cancer-specific monoclonal antibody recognizes the aberrantly glycosylated podoplanin.
Kato Y, Kaneko MK.
Sci Rep. 2014 Aug 1;4:5924. PMID: 25080943
Chromosome Transfer
A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function.
Kononenko AV, Bansal R, Lee NC, Grimes BR, Masumoto H, Earnshaw WC, Larionov V, Kouprina N.
Nucleic Acids Res. 2014 Dec 1;42(21). PMID: 25260588
Nuclear transfer
Assessment of nuclear transfer techniques to prevent the transmission of heritable mitochondrial disorders without compromising embryonic development competence in mice.
Neupane J, Vandewoestyne M, Ghimire S, Lu Y, Qian C, Van Coster R, Gerris J, Deroo T, Deforce D, De Sutter P, Heindryckx B.
Mitochondrion. 2014 Sep;18:27-33. PMID: 25229667
Mitochondria transfer
Cell fusion through a microslit between adhered cells and observation of their nuclear behavior.
Wada K, Hosokawa K, Kondo E, Ito Y, Maeda M.
Biotechnol Bioeng. 2014 Jul;111(7):1464-8. PMID: 24420735
Nuclear transfer
Embryonic development following somatic cell nuclear transfer impeded by persisting histone methylation.
Matoba S, Liu Y, Lu F, Iwabuchi KA, Shen L, Inoue A, Zhang Y.
Cell. 2014 Nov 6;159(4):884-95. PMID: 25417163
Analysis of cellular function
A biotin-streptavidin-biotin bridge dramatically enhances cell fusion.
Li J, Yu X, Wagner TE, Wei Y.
Oncol Lett. 2014 Jul;8(1):198-202. PMID: 24959245
Analysis of cellular function
Transitory FGF treatment results in the long-lasting suppression of the proliferative response to repeated FGF stimulation.
Poole A, Knowland N, Cooper E, Cole R, Wang H, Booth L, Kacer D, Tarantini F, Friesel R, Prudovsky I.
J Cell Biochem. 2014 May;115(5):874-88. PMID: 24375433
Nuclear transfer
Nuclear reprogramming by interphase cytoplasm of two-cell mouse embryos.
Kang E, Wu G, Ma H, Li Y, Tippner-Hedges R, Tachibana M, Sparman M, Wolf DP, Scholer HR, Mitalipov S.
Nature. 2014 May 1;509(7498):101-4. PMID: 24670652
Somatic cell nuclear transfer
MⅡ spindle transfer
Therapeutic potential of somatic cell nuclear transfer for degenerative disease caused by mitochondrial DNA mutations.
Greggains GD et al
Scientific Reports, 4, 3844 (2014). PMID: 24457623
Somatic cell nuclear transfer
Establishment of totipotency does not depend on Oct4A.
Wu G et al
Nature Cell Biol., 15, 1089–1097(2013). PMID: 23934214
Preparation of monoclonal antibodies
Development of an enzyme immunoassay using a monoclonal antibody against the psychoactive diterpenoid salvinorin A.
Paudel MK et al
J. Nat. Prod., 76 (9), 1654-1660 (2013). PMID: 23987562
Nuclear transfer
Human embryonic stem cells derived by somatic cell nuclear transfer.
Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-Hedges R, Ma H, Kang E, Fulati A, Lee HS, Sritanaudomchai H, Masterson K, Larson J, Eaton D, Sadler-Fredd K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer RL, Wolf D, Mitalipov S.
Cell. 2013 Jun 6;153(6):1228-38 PMID: 23683578
Nuclear transfer
Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants.
Paull D, Emmanuele V, Weiss KA, Treff N, Stewart L, Hua H, Zimmer M, Kahler DJ, Goland RS, Noggle SA, Prosser R, Hirano M, Sauer MV, Egli D.
Nature. 2013 Jan 31;493(7434):632-7. PMID: 23254936
Chromosome Transfer
Towards germline gene therapy of inherited mitochondrial diseases.
Tachibana M, Amato P, Sparman M, Woodward J, Sanchis DM, Ma H, Gutierrez NM, Tippner-Hedges R, Kang E, Lee HS, Ramsey C, Masterson K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer R, Mitalipov S.
Nature. 2013 Jan 31;493(7434):627-31. PMID: 23103867
Microcell mediated chromosome transfer
Protecting a transgene expression from the HAC-based vector by different chromatin insulators.
Lee NC et al.
Cell Mol. Life Sci.,70 (19), 3723-3737 (2013).PMID: 23677492
Microcell mediated chromosome transfer 
Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts.
J Radiat Res. et al. 
J. Radiat. Res., 54 (6) 989-997 (2013). PMID: 23674607
Analysis of cellular function 
Acquired resistance to EGFR inhibitors is associated with a manifestation of stem cell-like properties in cancer cells.
 Shien K et al.
Cancer Res.,73(10), 3051–3061 (2013). PMID: 23542356
Analysis of cellular function
COG complexes form spatial landmarks for distinct SNARE complexes. 
Willett R. et al 
Nat. Commun., 4, 1553 (2013).PMID: 23462996
Premature chromosome condensation (PCC)
Influence of track directions on the biological consequences in cells irradiated with high LET heavy ions. 
Fujii Y.et al. 
Int. J. Radiat. Biol.,89(6), 401-410 (2013). PMID: 23363030
Preparation of monoclonal antibodies
 Establishment of novel monoclonal antibodies KMab-1 and MMab-1 specific for IDH2 mutations. 
Kaneko MK. et al.
Biochem. Biophys. Res. Commun.,432(1), 40-45 (2013). PMID: 23376717
Somatic cell nuclear transfer 
Valproic acid treatment from the 4-cell stage improves Oct4 expression and nuclear distribution of histone H3K27me3 in mouse cloned blastocysts.
lsaji Y. et al.
J. Reprod. Dev., 59(2), 196-204 (2013).PMID: 23337102
Analysis of cellular function 
Tetraspanin is required for generation of reactive oxygen species by the dual oxidase system in Caenorhabditis elegans. 
Moribe H. et al.
PLoS Genet., 8(9), e1002957 (2012). PMID: 23028364
Pronuclear Transplantation
Production of offspring after sperm chromosome screening: an experiment using the mouse model.
Watanabe H, Kusakabe H, Mori H, Yanagimachi R, Tateno H.
Hum Reprod. 2013 Feb;28(2):531-7. PMID: 23136143
Preparation of monoclonal antibodies
Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin. 
Kaji C. et al. 
Acta. Histochem. Cytochem., 45(4), 227-237 (2012). PMID: 23012488
Preparation of monoclonal antibodies
PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells. 
Shibata T. et al.
Int. Immunol., 24(10), 613-623 (2012). PMID: 22836022
Mitochondrial transfer
Transferring isolated mitochondria into tissue culture cells.
Yang YW. et al.
Nucleic Acids Res., 40(19), e148 (2012). PMID: 22753025
Heteroplasmic oocytes 
Rapid mitochondrial DNA segregation in primate preimplantation embryos precedes somatic and germline bottleneck. 
Lee HS. Et al. 
Cell Reports 1(5), 506-515 (2012). PMID: 22701816
Preparation of monoclonal antibodies
Immunohistochemical detection of IDH1 mutation, p53, and internexin as prognostic factors of glial tumors. 
Takano S. et al.
J. Neurooncol.,108(3), 361-373 (2012). PMID: 22396072
Preparation of monoclonal antibodies
HIG1, a novel regulator of mitochondrial γ-secretase, maintains normal mitochondrial function.
Hayashi H. et al. 
FASEB J., 26(6), 2306-2317 (2012). PMID: 22355194
Microcell-mediated chromosome transfer 
Dysregulation of gene expression in the artificial human trisomy cells of chromosome 8 associated with transformed cell phenotypes. 
Nawata H. et al.
PLoS One, 6(9), e25319 (2011). PMID: 21980425
Reprogramming of somatic cells
Human oocytes reprogram somatic cells to a pluripotent state. 
Noggle S. et al.
Nature, 478, 70–75 (2011). PMID: 21979046
GV transplantation
The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model. 
Comizzoli P. et al. 
Hum. Reprod., 26(8), 2165-2177 (2011). PMID: 21665874
Analysis of cellular function
Stem cell induced cardiac regeneration: fusion/mitochondrial exchange and/or transdifferentiation? 
Song YH. et al.
Cell Cycle, 10(14), 2281-2286 (2011). PMID: 21654195
Pronuclear transfer 
Birth of normal mice following round spermatid injection without artificial oocyte activation. 
Ogonuki N. et al.
J. Reprod. Dev., 57, 534-538 (2011). PMID: 21441713
GV transplantation
Involvement of mouse nucleoplasmin 2 in the decondensation of sperm chromatin after fertilization.
Inoue A. et al.
Biol. Reprod., 85, 70-77 (2011). PMID: 21415138
Preparation of monoclonal antibodies
Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation. 
Kaneko MK. et al.
Biochem. Biophys. Res. Commun., 406(4), 608-613 (2011). PMID: 21352804
Analysis of cellular function
Human adipose tissue-derived stem cells exhibit proliferation potential and spontaneous rhythmic contraction after fusion with neonatal rat cardiomyocytes.
Metzele R. et.al. 
FASEB J., 25(3), 830-839 (2011). PMID: 21059751
Premature chromosome condensation (PCC)
A comparison of chromosome repair kinetics in G(0) and G(1) reveals that enhanced repair fidelity under noncycling conditions accounts for increased potentially lethal damage repair.
Liu C. et al.
Radiat. Res.,174(5), 566-573 (2010). PMID: 20954858
Preparation of monoclonal antibodies [Immunogen:recombinant feline cystatin C]
Molecular cloning, expression in Escherichia coli, and development of monoclonal antibodies to feline cystatin C. 
Nakata J. et al. 
Vet. Immunol. Immunopathol., 138(3), 231-234 (2010). PMID: 20739069
Reprogramming of somatic cells
Comparison of reprogramming efficiency between transduction of reprogramming factors, cell-cell fusion, and cytoplast fusion.
Hasegawa K. et al.
Stem Cells. 28(8), 1338-1348 (2010). PMID: 20572011
Preparation of monoclonal antibodies
Production of rat monoclonal antibodies against RNA-binding protein Hzf.
Nakamura H. et al.
Hybridoma (Larchmt), 29(1), 7-11 (2010). PMID: 20199145
Mitochondria transfer
Chromosome transfer in mature oocytes.
Tachibana M, Sparman M, Mitalipov S.
Nat Protoc. 2010 Jun;5(6):1138-47. PMID: 20539289
Pronuclear Transplantation
Pronuclear transfer in human embryos to prevent transmission of mitochondrial DNA disease.
Craven L, Tuppen HA, Greggains GD, Harbottle SJ, Murphy JL, Cree LM, Murdoch AP, Chinnery PF, Taylor RW, Lightowlers RN, Herbert M, Turnbull DM.
Nature. 2010 May 6;465(7294):82-5. PMID: 20393463
Reprogramming of somatic cells
Reprogramming of somatic cells induced by fusion of embryonic stem cells using hemagglutinating virus of Japan envelope (HVJ-E). 
Yue XS. Et al.
Biochem. Biophys. Res. Commun., 394(4), 1053-1057 (2010). PMID: 20338150
Reprogramming of somatic cells
Generation of induced pluripotent stem cells by efficient reprogramming of adult bone marrow cells.
Kunisato A
Stem Cells Dev., 19(2), 229-238 (2010). PMID: 19558219
Preparation of monoclonal antibodies [Immunogen: lacto-series gangliosides 30-isoLM1and 30,60-isoLD1]
GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.
Kato Y. et al.
Biochem. Biophys. Res. Commun., 391(1), 750-755 (2010).PMID: 19944071
Analysis of cellular function
A cellular restriction dictates the permissivity of nondividing monocytes/macrophages to lentivirus and gammaretrovirus infection.
Kaushik R. et al.
Reproduction, 137(4), 625-632 (2009). PMID: 19616766
Preparation of monoclonal antibodies [Immunogen: synthetic human IDH1R132H peptide]
A monoclonal antibody IMab-1 specifically recognizes IDH1R132H, the most common glioma-derived mutation.
Kato Y. et al.
Biochem. Biophys. Res. Commun., 390(3), 547-551 (2009). PMID: 19818334
Nuclear transfer
Meiotic maturation failure induced by DICER1 deficiency is derived from primary oocyte ooplasm.
Mattiske DM. et al.
Reproduction, 137(4), 625-632 (2009). PMID: 19129368
Cancer immunology
Anti-tumor effects of fusion cells of type 1 dendritic cells and Meth A tumor cells using hemagglutinating virus of Japan-envelope.
Yanai S. et al
Int. J. Oncol., 35(2), 249-255 (2009). PMID: 19578737
Analysis of cellular function
Endocytosis of flotillin-1 and flotillin-2 is regulated by Fyn kinase.
Riento K. et al.
Journal of Cell Science,122, 912- 918 (2009). PMID: 19258392
Analysis of cellular function
Maintenance and characterization of spontaneous contraction rhythm in cultured cardiac myocytes fused with cardiac fibroblasts.
Matsuyama D. et al.
Biosystems, 92(3), 226-232 (2008). PMID: 18423847
Mitochondrial gene replacement
Mitochondrial gene replacement in primate offspring and embryonic stem cells.
Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O, Mitalipov S.
Nature. 2009 Sep 17;461(7262):367-72. PMID: 19710649
GV transplantation
Inhibition of human immunodeficiency virus type 1 replication by regulated expression of a polymeric Tat activation response RNA decoy as a strategy for gene therapy in AIDS.
Lisziewicz J.et al.
Human Reproduction, 23, 1377-1384 (2008). PMID: 18367455
Preparation of monoclonal antibodies 
In vivo expansion of CD4+Foxp3+ regulatory T cells mediated by GITR molecules.
Nishioka T. et al.
Immunol,Lett, 121(2), 97-104 (2008). PMID: 18930767
Preparation of monoclonal [Immunogen : recombinant TARSH-CH protein]
Generation and characterization of novel monoclonal antibodies against murine and human TARSH proteins.
Uekawa N. et al.
Hybridoma (Larchmt), 26(6), 381-385 (2007).PMID: 18158782
Microcell mediated chromosome transfer
Microcell and HT1080 Microcell and hiMSC (immortalized human mesencymal stem cell)
S. Yamaguchi et al.
Chromosome Science , 9, 65-73 (2006).
Premature chromosome condensation (PCC)
Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the non-homologous end-joining pathway.
Okayasu R. et al.
Radiation Research, 165(1), 59-67 (2006). PMID: 16392963
Premature chromosome condensation (PCC)
Facilitated detection of chromosome break and repair at low levels of ionizing radiation by addition of wortmannin to G1-type PCC fusion incubation.
Okada M. et al.
Mutation Research, 562(1-2), 11-17 (2004). PMID: 15279826
Cancer immunology
Enhanced tumor-specific long-term immunity of hemagglutinating [correction of hemaggluttinating] virus of Japan-mediated dendritic cell-tumor fused cell vaccination by coadministration with CpG oligodeoxynucleotides.
Hiraoka K. et al.
Journal of Immunology, 173, 4297-4307 (2004). PMID: 15383558

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GenomONE-CF EX SeV-E (HVJ-E) 1 vial Cell Fusion Reagents

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.