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For determination of Rat hepatocyte growth factor [Mouse HGF]

Rat HGF EIA

Hepatocyte growth factor (HGF), which is a potent growth factor as a trigger for liver regeneration, act as accelerator for growth of various cells not only hepatocyte but also epithelial cells, endothelial cells, and some of mesenchymal tissue. Several studies have reported that HGF had correlation with tumor progression. It also established that HGF enhanced the motility of various types of cells, induced morphogenesis and tubule formation, and controlled the immune response, and many researchers have studied using mice or rats. This kit is developed for determination of rat hepatocyte growth factor (rat HGF) for research purposes. Rat HGF can be determined quantitatively using the rat HGF standard solution supplied with this kit. This kit also cross reacts with human HGF but its cross reactivity is about 4%.

Assay Principle

The detection system of this kit is of a sandwich configuration based on enzyme immuno assay consisting of three steps of antigen-antibody reaction between a monoclonal antibody raised against genetically engineered human HGF and most cross-reactive with rat HGF (anti-rat HGF mouse monoclonal antibody), rat HGF in samples, anti-rat HGF rabbit antibody and anti-rabbit goat immunoglobulin, followed by color development by enzyme reaction. The 1st reaction takes place between the anti-rat HGF mouse monoclonal antibody immobilized on a solid phase microplate and rat HGF in samples; the 2nd reaction between rat HGF in samples bound to the solid plate antibody and anti-rat HGF rabbit polyclonal antibody, and the 3rd reaction between the enzyme labeled antibody (peroxidase labeled anti-rabbit goat immunoglobulin) and the anti-rat HGF rabbit polyclonal antibody. After the 3rd reaction, color proportional to the rat HGF concentration is developed by enzyme reaction and its absorbance is measured for the determination of rat HGF on a calibration curve prepared using the rat HGF standard solution

Kit Contents

1 Solid phase microplate (8 wells/strip x 12)
(Coated with anti-rat HGF monoclonal antibody)
1 ea.
2 Rat HGF standard solution
(1 vial each of 0, 0.4, 1.0, 3.0, 10 and 25 ng/mL standard concentration: 0.5mL/vial)
6 vials
3 Sample diluent L, low salt concentration 15 mL x 1 vial
4 Sample diluent H, high salt concentration 15 mL x 1 vial
5 Anti-rat HGF rabbit antibody
(Anti-rat HGF rabbit polyclonal antibody)
10 mL x 1 vial
6 Enzyme labeled antibody
(Peroxidase labeled anti-rabbit goat immunoglobulin)
10 mL x 1 vial
7 Enzyme substrate solution
(Sodium perborate, tetrahydrate)
30 mL x 1 vial
8 Color developer
(o-phenylenediamine, dihydrochloride)
4 tablets
9 Reaction stopper solution
(19.6% sulfuric acid)
5 mL x 1 vial
10 Washing solution (20 times concentrated) 25 mL x 3 vials
11 Plate seal 3 ea.

<Reference>
1. N. Nakayama, et al: Prediction of prognosis in LEC rats with fulminant hepatitis. ACTA HEPATOLOGICA JAPONICA 31: 363-369, 1994
2. Yamada A, et al: Rapid and sensitive enzyme-linked immunosorbent assay for measurement of HGF in rat and human tissues. Biomedical Research 16 (2): 105-114, 1995.

References

1. N. Nakayama, et al: Prediction of prognosis in LEC rats with fulminant hepatitis. ACTA HEPATOLOGICA JAPONICA 31: 363-369, 1994
2. Yamada A, et al: Rapid and sensitive enzyme-linked immunosorbent assay for measurement of HGF in rat and human tissues. Biomedical Research 16 (2): 105-114, 1995.

1. N. Nakayama, et al: Prediction of prognosis in LEC rats with fulminant hepatitis. ACTA HEPATOLOGICA JAPONICA 31: 363-369, 1994 2. Yamada A, et al: Rapid and sensitive enzyme-linked immunosorbent assay for measurement of HGF in rat and human tissues. Bi

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

< Reagent related to HGF(Hepatocellular Growth Factor) >