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For determination of mouse hepatocyte growth factor [Mouse HGF]

MOUSE HGF EIA

Background

Hepatocyte growth factor (HGF), which is a potent growth factor as a trigger for liver regeneration, act as accelerator for growth of various cells not only hepatocyte but also epithelial cells, endothelial cells, and some of mesenchymal tissue. Several studies have reported that HGF had correlation with tumor progression. It also established that HGF enhanced the motility of various types of cells, induced morphogenesis and tubule formation, and controlled the immune response, and many researchers have studied using mice and rats. This kit is developed for determination of mouse hepatocyte growth factor (mouse HGF) for research purposes. Mouse HGF can be determined quantitatively sing the mouse HGF standard solution supplied with this kit. This kit also cross reacts with human HGF but its cross reactivity is about 4%.

Kit component

1 Solid phase microplate (8 wells/strip x 12)
(Coated with anti-HGF monoclonal antibody)
1 ea.
2 Mouse HGF standard solution
(1 vial each of 0, 0.4, 1.0, 3.0, 10 and 25 ng/mL standard concentration: 0.5mL/vial)
6 vials
3 Sample diluent L, low salt concentration 15 mL x 1 vial
4 Sample diluent H, high salt concentration 15 mL x 1 vial
5 Anti-HGF rabbit antibody
(Anti-HGF rabbit polyclonal antibody)
10 mL x 1 vial
6 Enzyme labeled antibody
(Peroxidase labeled anti-rabbit goat immunoglobulin)
10 mL x 1 vial
7 Enzyme substrate solution
(Sodium perborate, tetrahydrate)
30 mL x 1 vial
8 Color developer
(o-phenylenediamine, dihydrochloride)
4 tablets
9 Reaction stopper solution
(19.6% sulfuric acid)
5 mL x 1 vial
10 Washing solution (20 times concentrated) 25 mL x 3 vials
11 Plate seal 3 ea.

Assay Principle

The detection system of this kit is of a sandwich configuration based on enzyme immuno assay consisting of three steps of antigen-antibody reaction between a monoclonal antibody raised against genetically engineered human HGF and most cross-reactive with mouse HGF (anti-HGF mouse monoclonal antibody), mouse HGF in samples, anti-HGF rabbit antibody and anti-rabbit goat immunoglobulin, followed by color development by enzyme reaction.
 The 1st reaction takes place between the anti-HGF mouse monoclonal antibody immobilized on a solid phase microplate and mouse HGF in samples; the 2nd reaction between mouse HGF in samples bound to the solid plate antibody and anti-HGF rabbit polyclonal antibody, and the 3rd reaction between the enzyme labeled antibody (peroxidase labeled anti-rabbit goat immunoglobulin) and the anti-HGF rabbit polyclonal antibody. After the 3rd reaction, color proportional to the mouse HGF concentration is developed by enzyme reaction and its absorbance is measured for the determination of mouse HGF on a calibration curve prepared using the mouse HGF standard solution.

Product List

Product Name Cat# Quantity Price

Anti HGF (ALPHA-chain)

SIM-2ZCKHH1 0.5ML

¥ 64,000
$ 854
€ 640

Anti HGF (reducted ALPHA-chain)

SIM-2ZCKHH2 0.5ML

¥ 64,000
$ 854
€ 640

Anti HGF (BETA-chain)

SIM-2ZCKHH3 0.5ML

¥ 64,000
$ 854
€ 640

Anti HGF (ALPHA-chain)

SIM-2ZCKRH1 0.5ML

¥ 64,000
$ 854
€ 640