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Even staining, high sensitivity detection, low background, high contrast bands

2D-SILVER STAIN-II - for Electrophoresis -

Background

For detection of proteins and nucleic acids migration by polyacrylamide gel electrophoresis, silver staining is now being highlighted as a sensitive staining method. However the original silver staining method is not necessarily easy to work and takes long-time. 2D-SILVER STAIN-II is an improved reagent kit developed to provide simple and fast staining.

 

 

Application

2D-SILVER STAIN II is applicable to the detection of proteins and nucleic acids subjected to polyacrylamide and SDS-polyacrylamide slab gel electrophoresis.

Running Condition
• Loading buffer: Tris SDS
• Sample: PageRuler™ Prestained Protein Ladder #SM1811(Fermentas UAB)

(1) x4 (6) x128
(2) x8 (7) x256
(3) x16 (8) x512
(4) x32 (9) x1024
(5) x64  
(Each Lane 3)

• Electrode buffer: SDS-Tris-Glycine Electrode buffer
• Running time:30 mA constant current/sheet、About 50 min.
• Dying Method: 2D-Silver Stain

Feature and Advantages

1. Rapid staining results in a short time after electrophoresis.
2. Staining with higher sensitivity For proteins:50 to 100 times more sensitive than CBB staining For nucleic acids: 50 to 100 times more sensitive than EB staining
3. Simple preparation and simple operation.
4. No use of such materials as heavy metals controlled by regulations.
5. Capable of restaining CBB-stained gels. (Double-staining)
6. Staining process can be stopped any time during development for desired chromatic figures.

Components

Regents Main ingredients Volume
1) Fixing Reagent Thiourea 100mLx1
2) Pretreatment reagent Dithiothreitol, Glutar-aldehyde, Thiourea 100mLx1
3) Staining Solution A Siver nitrate 100mLx1
4) Staining Solution B Ammonium hydroxide, Sodium hydroxide 100mLx1
5) Concentrated developer Citric acid, Folmaldehyde, Sodium hydroxide 100mLx1
6) Stopper Citric acid 100mLx1
References

1. K. Ohsawa et. Al.: Silver Stain for Detecting 10-Femotogram Quantities of protein after Polyacrylamide Gel Electrophoresis, Anal. Biochem., 135, 409(1983)
2. S. Irie: A highly sensitive silver Staining for detection of proteins in polyacrylamide gels, biochemistry(Japan), 52, 411(1980)
3. B.R. Oakley et. al.: Visualization of proteins with silver “stain” for detecting proteins in polyacrylamide gels, Anal. Biochem., 105, 361(1980)
4. H.M. Poehling et. al.: Visualization of proteins with silver ”stain”, a critical analysis, electrophoresis, 2, 141 (1981)
5. U.K. Laemmli: Cleavage of structural proteins during the assembly of the head of bacteriophage T4., Nature, 227, 680(1970)

Product List

Product Name Cat# Quantity Price

2D-Silver Stain Reagent II [For 10 gels ]

DCB-423413 1PACK

¥ 9,000
$ 120
€ 90

1. K. Ohsawa et. Al.: Silver Stain for Detecting 10-Femotogram Quantities of protein after Polyacrylamide Gel Electrophoresis, Anal. Biochem., 135, 409(1983) 2. S. Irie: A highly sensitive silver Staining for detection of proteins in polyacrylamide gels,