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AteloCell® Series are perfectly suitable for cell culture from daily cell maintenance to fundamental research of regenerative medicine.

AteloCell® series Frequently Asked Questions (FAQ)

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AteloCollagen sponge for 35mm culture dish

Question Is the collagen sponge thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer We have not measured the thermal-resistant of this product.
For your information, since liquid collagen denatures around 40°C, the sponge type collagen may not be denatured until more high temperature.
Question What is the elasticity of this collagen? Will it torn easily? How much weight can it endure? Most importantly, how careful and delicate we should be while handling the collagen?
Answer We have not tested the elasticity of this product.
However, it might resist a certain level of load because this product is freeze dried insoluble collagen.
Question Is it suitable for transplantation, for example skin transplant for wound healing?
Answer This is not suitable for transplantation because this is native collagen which has not eliminated telopeptide.
Question Will the collagen sponge dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation (such as skin cells) onto/into animal models? What will happen to the cultured cells when the collagen dissolved?
Answer This product is made from insolble collagen, so it does not dissolve easily.
In vivo situation, it is dissolved from MMP etc.
Although this product is not suitable to transplantation, there is a report which this product is used for in vivo experiment. According to the report, the collagen removed from live body after 8 weeks.
It is considered that transplanted collagen would replace to extracelluar matrix from transplanted cell until it is disappeared.
Question Can we cut the collagen sheet to smaller sizes manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the collagen sheet/structure/integrity during and after cutting?
Answer You can cut the collagen sheet, but the pore structure might be crumpled up if you use blunt cutter.
Question How strongly/intact will the cells adhere/attach to the collagen sponge? Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question How could we detach the cells from the collagen sponge with minimal damage?
Answer Please use collagenase for detach the collagen.

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Atelocollagen Sponge, 90 x 80 x 5mm

Question What is the thickness of the atelocollagen sponge after absorbing water (deform)?
Answer It will become slightly thin less than 5 mm.
Question The sponge will deform once absorbs water unless cross-link is performed. What is meant by cross-link in this aspect?
Answer Physical cross-link by UV or γ radiation or chemical cross-link by cross-linker are considered.
Question Is the atelocollagen sponge thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer We have not measured the thermal-resistant of this product. For your information, since liquid collagen denatures around 40°C, the sponge type collagen may not be denatured until more high temperature.
Question What is the elasticity of this collagen? Will it torn easily? How much weight can it endure? Most importantly, how careful and delicate we should be while handling the collagen?
Answer We have not tested the elasticity of this product.
However, it might resist a certain level of load because this product is freeze dried insoluble collagen.
Question Is it suitable for transplantation, for example skin transplant for wound healing?
Answer You can use this product for transplantation if you cross-link the collagen.
Question Will the atelocollagen sponge dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation (such as skin cells) onto/into animal models? What will happen to the cultured cells when the collagen dissolved?
Answer The sponge structure will be broken with no cross-link.
We don't have the information of the time until disappear of collagen after transplantation. But it would hold the structure for several month with strong cross-link.
Question Can we cut the collagen sheet to smaller sizes manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the collagen sheet/structure/integrity during and after cutting?
Answer You can cut the collagen sheet, but the pore structure might be crumpled up if you use blunt cutter.
Question How strongly/intact will the cells adhere/attach to the collagen sponge? Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question How could we detach the cells from the collagen sponge with minimal damage?
Answer Please use collagenase for detach the collagen.

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Atelocollagen, Permeable membrane

Question Is the membrane thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer We have not measured the thermal-resistant of this product.
For your information, since liquid collagen denatures around 40C, the film type collagen may not be denatured until more high temperature.
Question What is the elasticity of the membrane? Will it torn easily? How much weight can it endure? Most importantly, how careful and delicate we should be while handling the membrane?
Answer We have not tested the elasticity of this product.
However, it would tear easily because this product is reprocessed to film form.
Question Is it suitable for transplantation, for example skin transplant for wound healing?
Answer yes, it is.
Question Will the permeable membrane dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation (of skin cells) onto animal models? What will happen to the cultured cells when the collagen dissolved?
Answer We consider that the permeable membrane would dissolve around one month after transplantation.
Question Can we cut the membrane to smaller sizes manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the membrane structure/integrity during and after cutting?
Answer Yes, you can cut the membrane to smaller sizes.
Question How strongly/intact will the cells adhere/attach to the permeable membrane, especially when we perform sandwich culture of two different cell types on the double side of the membrane? Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question How could we detach the cells from the two-sided membrane with minimal damage?
Answer Please recover the cells by scraper or by collagenase.
Question How to view two different cell types on two different surface of the membrane? Do we flip over with forceps? Will the action cause damage or dislodge the cells?
Answer You can observe the cells by phase-contrast microscope.
However, it is difficult to discriminate which sides the cells were seeded.
Therefore, it would be clear that cells are labeled with fluorescein and observe by fluorescence microscope.
Question Is the membrane for 50mm culture dish, 6- and 24-well culture plate detachable from the plastic ware?
Answer You detach it by knife.

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Atelocollagen, Honeycomb sponge

Question How to ensure that the cultured cells adhered to honeycomb sponge? Can we view it under microscope?
Answer Phase-contrast microscope enables to observe.
Question Is the honeycomb sponge thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer We have not measured the thermal-resistant of this product.
For your information, since liquid collagen denatures around 40°C, the sponge type collagen may not be denatured until more high temperature.
Question What is the durability of the honeycomb sponge? How much weight can it endure?
Answer We have not tested the elasticity of this product.
However, it would be break by load because this product is freeze dried low concentration atelocollagen.
Question Will the honeycomb sponge dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation (such as skin cells) onto/into animal models? What will happen to the cultured cells when the honeycomb sponge dissolved?
Answer The sponge will dissolve around one month after transplantation.
Question Can we cut the honeycomb sponge to smaller sizes (thinner) manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the sponge structure/integrity during and after cutting?
Answer You can cut the collagen sheet, but the pore structure might be crumpled up if you use blunt cutter.
Question How strongly/intact will the cells adhere/attach to the honeycomb sponge? Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question Cells can be easily harvested by collagenase treatment. Will the treatment affect cells viability? Any protocol for the treatment?
Answer Please add collagenase to be 0.1% final concentration and dissolve about 30 min.
If you worry about cell damage, rise the collagenase concentration and reduce the processing time.

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Atelocollagen sponge (MIGHTY)

Question Is the MIGHTY sponge thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer We have not measured the thermal-resistant of this product.
For your information, since liquid collagen denatures around 40°C, the sponge type collagen may not be denatured until more high temperature.
Question Will the MIGHTY sponge dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation (such as skin cells) onto/into animal models? What will happen to the cultured cells when the MIGHTY dissolved?
Answer Since MIGHTY sponge is a high intensity sponge, it hard to dissolve.
There is a data that indicate MIGHTY sponge is not dissolved at least 3 months after transplantation.
Question Can we cut the MIGHTY sponge to smaller sizes in terms of thickness manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the MIGHTY structure during and after cutting? Any recommended steps or precautions to be taken during cutting?
Answer You can cut the MIGHTY sponge when it is swellheaded state.
However, the knife might be nicked after cutting some sponges because this is a high intensity sponge.
Question How strongly/intact will the cells adhere/attach to the MIGHTY sponge?
Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question How could we detach the cells from the MIGHTY sponge with minimal damage?
Answer Since MIGHTY sponge is an high intensity, it's difficult to recover live cells from the sponge.
On the one hand, recovering nucleotide or protein after homogenizing is available.

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Collagen microspheres

Question What’s the supernatant/solution in microsphere collagen?
Answer PBS
Question How to ensure that the cultured cells adhered to the microspheres? Can we view it under microscope?
Answer You can observe it by phase-contrast microscope.

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Atelocollagen coated B-TCP (B-calcium phosphate) scaffold

Question What is the thickness of this scaffold?
Answer 1.0±0.1 mm
Question Is the scaffold thermal-resistant? What is the maximum temperature it can withstand? Will it degrade when the temperature increases for an example above body temperature?
Answer The β-TCP scaffold can resist high temperature, but the coated collagen will be denatured around 40°C.
Question What is the elasticity of the scaffold? Will it torn/break easily? How much weight can it endure? Most importantly, how careful and delicate we should be while handling the collagen?
Answer We have not tested the elasticity of this product.
However, we consider that the scaffold is from β-TCP and it can resist a load.
Question Is it suitable for transplantation?
Answer Yes. But this product is designed to bone formation assay.
Question Will the scaffold dissolved? How would they get dissolved? How long does it take to get them dissolved completely, especially after transplantation onto/into animal models? What will happen to the cultured cells when the scaffold dissolved?
Answer It's difficult to dissolve because this product is made from β-TCP.
Question Can we cut the scaffold to smaller sizes manually probably with a Scalpel? Will it cause disruption/distortion/dispersion of the scaffold structure/integrity during and after cutting?
Answer It's difficult to cut because this product is made from β-TCP.
Question How strongly/intact will the cells adhere/attach to the scaffold? Will the cells detach easily even after strong agitation?
Answer The cells don't detach easily even after strong agitation once they are attached unless low adhesiveness cells.
Question How could we detach the cells from the scaffold with minimal damage?
Answer Please use collagenase for detach the collagen.

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