Chondrocytes produce extracellular matrix such as collagen, mucopolysaccharide and so on. There are conventional methods to measure acidic mucopolysaccharides using radioisotopes but the area and the amount used regarding radioisotopes are restricted. On the other hand, HPLC analysis is complicated to maintain the system and to extra for making samples. Acidic Mucopolysaccharide Assay Kit (PMC-AK03-COS) provides a convenient system for mucopolysaccharides visualization using Stains All, which combines with acidic mucopolysaccharides, with ease. Stains All normally combines with acidic substance, however the Stains All in this kit stains only acidic mucopolysaccharides of chondrocytes selectively.
|Staining Solution||4 mL||4-10ºC|
|Enzyme Reagent||5 vials||4-10ºC|
|Standard Stock Solution (100 ug/mL Chondroitin Sulfate Calibrator)||2 mL||4-10ºC|
One kit contains regents for 100 (20 samples x 5 times) samples
This kit cannot quantify various glycosaminoglycans individually
This kit cannot quantify the mucopolysaccharides in culture medium
[Additional Materials Required]
Fig.1 Acidic Mucopolysaccharide and acidic substances detected by Acidic Mucopolysaccharide Assay Kit
This kit stains only acidic mucopolysaccharides of chondrocytes selectively.
Fig.2 Cells form spheroid clusters under Hypoxia condition
Prepare cultured chondrocytes and cells are detached by the treatment of trypsin solution. Transfer detached cells to 15 mL centrifuge tube and centrifuge the centrifuge tube. Remove supplement and add 5 mL of medium to the centrifuge tube. In about 3 weeks, cells will form cartilage-like spheroids.
Acidic Mucopolysaccaride Assay Kit