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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti IRF3 [Interferon regulatory factor 3] monoclonal antibody (clone : IRF35I218)

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of human IRF3 by Western blot. Samples: Whole cell lysate (25 μg) from HT1080 cells. [Lot No. IRF35I218-2]

Predicted molecular weight: 47 kDa

Western_blot_protocolPDF

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-IRF3 mAb. [Lot No. IRF35I218-2]

Predicted molecular weight: 47 kDa

Immunoprecipitation protocolPDF

[Flow cytometry]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-IRF3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. IRF35I218-2]

Flow cytometry protocolPDF

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-IRF3 mAb. [Lot No. IRF35I218-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti IRF3

CBX-CBX00167 100UG

¥ 39,000
$ 520
€ 390