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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti TIP49A [RUVBL1, RVB1, ECP54,TIP49, NMP238] monoclonal antibody (clone : 2943C1a)

Background

Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. [UniProtKB Function]

Application

[Western Blot]
Detection of human RUVBL1 by Western blot. Samples: Whole cell lysate (50 μg) from HT-1080 cells. [Lot No. 2943C1a-1]

Predicted molecular weight: 50 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1]

Immunoprecipitation protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti RUVBL1

CBX-CBX00431 100UG

¥ 39,000
$ 520
€ 390