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Monoclonal Antibodies to Transcription Factors and Nuclear DNA Binding Proteins

Anti DDX5 [p68, HLR1, G17P1, HUMP68, DKFZp686J01190] monoclonal antibody (clone : 2257C3a)

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [NCBI Entrez Gene Summary]

Application

[Western Blot]
Detection of DDX5 by Western blot. Samples: Whole cell lysate from human HeLa (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa

Western_blot_protocolPDF

 

[Immunoprecipitation]
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DDX5 mAb. [Lot No. 2257C3a-1]

Predicted molecular weight: 69 kDa

Immunoprecipitation protocolPDF

 

[Immunocytochemistry]
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX5 mAb. [Lot No. 2257C3a-1]

Immunocytochemistry protocolPDF

Product List

Product Name Cat# Quantity Price

Anti DDX5

CBX-CBX00460 100UG

¥ 39,000
$ 520
€ 390