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Useful for the chromatin research on the drosophila.

Anti Drosophila SSRP1 Polyclonal antibody
Anti Drosophila SPT16 Polyclonal antibody

Background

 dSSRP1 and dSPT16 are a Drosophila counterpart of the FACT [Facilitates Chromatin Transcription] subunit. FACT consists of a heterodimer of SSRP1 and SPT16 and was identified as a factor that facilitates chromatin transcription in vitro. Further studies revealed that the factor also facilitates various chromatin transactions such as transcriptional regulation, DNA replication and histone replacement. The antibodies against dSSRP1 and dSPT16 are powerful tool for Western blot analyses, immunostaining, immunoprecipitation and chromatin immunopreciptation in studies using Drosophila.

Application

1. Western blotting : 1/4,000 to 1/8,000
    Predicted molecular weight dSSRP1 : 93kDa dSPT16 : 130kDa
2. Immunohistochemistry : 1/500 to 1/2,000
3. ELISA : 1/4,000 to 1/10,000
4. Immunoprecipitation : 0.5 to 2ul
5. Chromain immunoprecipitation, 1 to 4ul


Immunostaining of a polytene chromosome (anti -dSSRP1 antibody)

Immunostaining of a polytene chromosome (anti -dSPT16 antibody)

<Reference>
1. Shimojima, T. et al. Drosophila FACT contributesto Hox gene expression through physical and functional interactions with GAGA factor. Genes & Dev. 17,1605-1616, 2003
2. Saunders, A. et al. Tracking FACT and RNA Polymerase II Elongation Complex Through Chromatin in Vivo. Science, 301, 1094-1096, 2003
3. Nakayama, T. et al. Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading. Genes & Dev. 21, 552-561, 2007

Product List

Product Name Cat# Quantity Price

Anti SSRP1

CAC-NIG-L1-SHA1 100UL

¥ 50,000
$ 667
€ 500

Anti SPT16

CAC-NIG-L1-SHA2 100UL

¥ 50,000
$ 667
€ 500

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.