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DNA Damage Antibody Useful for research on DNA Damage and Repair

Anti Cyclobutane Pyrimidine Dimers (CPDs) [Clone : TDM2]


Monoclonal Antibodies against DNA Damage [PDF]

Go to "Antibodies against DNA Damage"

Background

DNA damage in cells exposed to ultraviolet (UV) radiation plays significant roles in cell-cycle arrest, activation of DNA repair, cell killing, mutation, and neoplastic transformation. The major types of DNA damage induced by UVB (280-315 nm, component of sunlight) and by UVC (200-280 nm) are cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts (6-4PPs), which are formed between adjacent pyrimidine nucleotides on the same strand of DNA. Approximately 70-80% of UV-induced DNA damage is CPDs and the remaining is 6-4PPs and Dewar isomer of 6-4PPs. These types of DNA lesions are repaired by nucleotide excision repair (NER) system in normal human cells. Mori et al (19) have established monoclonal antibodies specific for CPDs or 6-4PPs. These antibodies enable one to quantitate photoproducts in DNA purified from cultured cells or from the skin epidermis using an enzyme-linked immunosorbent assay (ELISA) and to visualize and measure photoproducts in DNA in cultured cells or the skin using indirect immunofluorescence (IIF). This technology would contribute to understanding of molecular mechanisms of cellular responses to UV and DNA damage in many research fields including cancer research, photobiology, dermatology, ophthalmology, immunology, and cosmetology.

Monoclonal Antibodies 
against DNA Damage

Source

The hybridoma was established by fusion of mouse myeloma cells with Balb/c mouse splenocytes immunized with methylated BSA conjugated with calf thymus DNA which was irradiated with UVC. This hybridoma (clone TDM-2) culture supernatant was collected and precipitated with ice-cold ammonium sulfate. After centrifugation, the pellet dissolved in small volume of double-distilled water was dialysed against PBS. The dialysate was then lyophilized.

Reactivity

1) The antibodies bind to CPDs in single-stranded DNA.
2) The antibodies bind to CPDs formed in every dipyrimidine sequence (TT, TC, CT and CC).
3) The antibodies stably binds to CPDs formed in oligonucleotides consisting of more than eight bases.

Cross Reactivity

The antibodies can bind to CPDs in denatured DNA from all organisms from bacteria to human.

Features

  • React specifically with damaged DNA Research applications include ELISA and IC
  • Useful for research into DNA damage repair mechanisms
  • Visualizes the DNA damage repair process
  • Applicable to a broad range of research interests:photobiology, optical medicine, cancer, etc.

Application

Immunocytochemistry; 1:1500 PROTOCOLS< Immunofluorescence microscopy >PDF
ELISA; 1:1000 PROTOCOLS<ELISA>PDF
Western blotting; Not tested
Immunoprecipitation; Not tested
Immunohistochemistry; Not tested
Flow cytometry; Not tested


Induction and repair of UV-induced DNA damage in normal human cells measured by ELISA


Visualization of UV-induced DNA damage by using immunofluorescence

Related Product

Cosmo Bio's new Cyclobutane Pyrimidine Dimer (CPD) ELISA Kit uses the highly sensitive and specific monoclonal anti-CPD antibody clone TDM-2, established by Mori et al. TDM-2 is widely cited in the literature and is considered to be the gold standard antibody for CPD detection and quantification.Cosmo Bio's CPD ELISA Kit is the first and only commercially available ELISA kit using TDM-2. All kits components are optimized for high sensitivity CPD detection from cultured cells or skin epidermis.


Go To "High Sensitivity CPD/ Cyclobutane Pyrimidine Dimer ELISA kit"

Product List

Product Name Cat# Quantity Price

Anti CPDs

CAC-NM-DND-001 1VIAL

¥ 44,000
$ 587
€ 440

High Sensitivity CPD ELISA kit Ver.2

CSR-NM-MA-K003 1KIT

¥ 100,000
$ 1334
€ 1000

Citation
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    Gene. 2017 Jun 29. pii: S0378-1119(17)30507-3. PMID: 28669926
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    Photochem Photobiol. 2017 Jan;93(1):315-322. PMID: 27861979
  • Photo-enzymatic repair of UVB-induced DNA damage in the two-spotted spider mite Tetranychus urticae.
    Murata Y, Osakabe M.
    Exp Appl Acarol. 2017 Jan;71(1):15-34. PMID: 27873138
  • UV radiation-induced SUMOylation of DDB2 regulates nucleotide excision repair
    Chunhua Han Ran Zhao John Kroger Jinshan He Gulzar Wani Qi-En Wang Altaf A. Wani
    Carcinogenesis bgx076. 22 July 2017 PMID:
  • Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes.
    Zhao B, Shah P, Qiang L, He TC, Budanov A, He YY.
    Photochem Photobiol. 2017 Jan;93(1):375-381. PMID: 27463837
  • Time-Restricted Feeding Shifts the Skin Circadian Clock and Alters UVB-Induced DNA Damage.
    Wang H, van Spyk E, Liu Q, Geyfman M, Salmans ML, Kumar V, Ihler A, Li N, Takahashi JS, Andersen B.
    Cell Rep. 2017 Aug 1;20(5):1061-1072. PMID: 28768192
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    DNA Repair (Amst). 2017 Mar;51:79-84. PMID: 28185850
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    J Cell Physiol. 2017 May 2. PMID: 28463453
  • Stabilization of Ultraviolet (UV)-stimulated Scaffold Protein A by Interaction with Ubiquitin-specific Peptidase 7 Is Essential for Transcription-coupled Nucleotide Excision Repair.
    Higa M, Zhang X, Tanaka K, Saijo M.
    J Biol Chem. 2016 Jun 24;291(26):13771-9. PMID: 27129218
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    Zhao B, Qiang L, Joseph J, Kalyanaraman B, Viollet B, He YY.
    Genes Dis. 2016 Mar;3(1):82-87. PMID: 28066797
  • Oral Administration of Fermented Soymilk Products Protects the Skin of Hairless Mice against Ultraviolet Damage.
    Kano M, Kubota N, Masuoka N, Hori T, Miyazaki K, Ishikawa F.
    Nutrients. 2016 Aug 20;8(8). PMID: 27556484
  • Photosensitized 2-amino-3-hydroxypyridine-induced mitochondrial apoptosis via Smac/DIABLO in human skin cells.
    Goyal S, Amar SK, Dwivedi A, Mujtaba SF, Kushwaha HN, Chopra D, Pal MK, Singh D, Chaturvedi RK, Ray RS.
    Toxicol Appl Pharmacol. 2016 Apr 15;297:12-21. PMID: 26933830
  • Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.
    Boros G, Kariko K, Muramatsu H, Miko E, Emri E, Heged?s C, Emri G, Remenyik E.
    Methods Mol Biol. 2016;1428:219-28. PMID: 27236802
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    Qiang L, Zhao B, Shah P, Sample A, Yang S, He YY.
    Autophagy. 2016;12(2):357-68. PMID: 26565512
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    Ming M, Zhao B, Qiang L, He YY.
    Photochem Photobiol. 2015 Jan-Feb;91(1):242-7. PMID: 25039758
  • Phloroglucinol enhances the repair of UVB radiation-induced DNA damage via promotion of the nucleotide excision repair system in vitro and in vivo.
    Piao MJ, Ahn MJ, Kang KA, Kim KC, Cha JW, Lee NH, Hyun JW.
    DNA Repair (Amst). 2015 Apr;28:131-8. PMID: 25766644
  • DNA Ligases I and III Support Nucleotide Excision Repair in DT40 Cells with Similar Efficiency.
    Paul-Konietzko K, Thomale J, Arakawa H, Iliakis G.
    Photochem Photobiol. 2015 Sep-Oct;91(5):1173-80. PMID: 26131740
  • Protective Effect of Diphlorethohydroxycarmalol against Ultraviolet B Radiation-Induced DNA Damage by Inducing the Nucleotide Excision Repair System in HaCaT Human Keratinocytes.
    Piao MJ, Hewage SR, Han X, Kang KA, Kang HK, Lee NH, Hyun JW.
    Mar Drugs. 2015 Sep 2;13(9):5629-41. PMID: 26404324
  • Role of AtMSH7 in UV-B-induced DNA damage recognition and recombination.
    Lario LD, Botta P, Casati P, Spampinato CP.
    J Exp Bot. 2015 Jun;66(11):3019-26. PMID: 25465032
  • Simultaneous detection of ultraviolet B-induced DNA damage using capillary electrophoresis with laser-induced fluorescence.
    Guthrie JW, Limmer RT, Brooks EA, Wisnewski C2, Loggins-Davis ND, Bouzid A.
    Anal Chim Acta. 2015 Jan 1;853:676-81. PMID: 25467517
  • SUMOylation of xeroderma pigmentosum group C protein regulates DNA damage recognition during nucleotide excision repair.
    Akita M, Tak YS, Shimura T, Matsumoto S, Okuda-Shimizu Y, Shimizu Y, Nishi R, Saitoh H, Iwai S, Mori T, Ikura T, Sakai W, Hanaoka F, Sugasawa K.
    Sci Rep. 2015 Jun 4;5:10984. PMID: 26042670
  • SUMO and ubiquitin-dependent XPC exchange drives nucleotide excision repair.
    van Cuijk L, van Belle GJ, Turkyilmaz Y, Poulsen SL, Janssens RC, Theil AF, Sabatella M, Lans H, Mailand N, Houtsmuller AB, Vermeulen W, Marteijn JA.
    Nat Commun. 2015 Jul 7;6:7499. PMID: 26151477
  • The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress.
    Lee MH, Wang L, Chang ZF.
    Nucleic Acids Res. 2014 Apr;42(8):4972-84. PMID: 24561807
  • E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis.
    Biswas AK, Mitchell DL, Johnson DG.
    Cancer Res. 2014 Jun 15;74(12):3369-77. PMID: 24741006
  • Loss of Caenorhabditis elegans BRCA1 promotes genome stability during replication in smc-5 mutants.
    Wolters S, Ermolaeva MA, Bickel JS, Fingerhut JM, Khanikar J, Chan RC, Schumacher B.
    Genetics. 2014 Apr;196(4):985-99. PMID: 24424777
  • Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules.
    Okazaki Y, Wang Y, Tanaka H, Mizuno M, Nakamura K, Kajiyama H, Kano H, Uchida K, Kikkawa F, Hori M, Toyokuni S.
    J Clin Biochem Nutr. 2014 Nov;55(3):207-15. PMID: 25411528
  • Detection of UV-induced mutagenic thymine dimer using graphene oxide.
    Chung CH, Kim JH, Chung BH.
    Anal Chem. 2014 Dec 2;86(23):11586-91. PMID: 25375800
  • Overexpression of rice OsREX1-S, encoding a putative component of the core general transcription and DNA repair factor IIH, renders plant cells tolerant to cadmium- and UV-induced damage by enhancing DNA excision repair.
    Kunihiro S, Kowata H, Kondou Y, Takahashi S, Matsui M, Berberich T, Youssefian S, Hidema J, Kusano T.
    Planta. 2014 May;239(5):1101-11. PMID: 24563249
  • ELF magnetic fields do not affect cell survival and DNA damage induced by ultraviolet B.
    Mizuno K, Narita E, Yamada M, Shinohara N, Miyakoshi J.
    Bioelectromagnetics. 2014 Feb;35(2):108-15. PMID: 24123106
  • Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity.
    Puumalainen MR, Lessel D, Ruthemann P, Kaczmarek N, Bachmann K, Ramadan K, Naegeli H.
    Nat Commun. 2014 Apr 28;5:3695. PMID: 24770583
  • Differential binding kinetics of replication protein A during replication and the pre- and post-incision steps of nucleotide excision repair.
    Gourdin AM, van Cuijk L, Tresini M, Luijsterburg MS, Nigg AL, Giglia-Mari G, Houtsmuller AB, Vermeulen W, Marteijn JA.
    DNA Repair (Amst). 2014 Dec;24:46-56. PMID: 25453469
  • ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis.
    Lario LD, Ramirez-Parra E, Gutierrez C, Spampinato CP, Casati P.
    Plant Physiol. 2013 Jun;162(2):1164-77. PMID: 23596192
  • Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams.
    Takano N, Takahashi Y, Yamamoto M, Teranishi M, Yamaguchi H, Sakamoto AN, Hase Y, Fujisawa H, Wu J, Matsumoto T, Toki S, Hidema J.
    J Radiat Res. 2013 Jul 1;54(4):637-48. PMID: 23381954
  • Non-invasive diffuse reflectance measurements of cutaneous melanin content can predict human sensitivity to ultraviolet radiation.
    Coelho SG, Zmudzka BZ, Yin L, Miller SA, Yamaguchi Y, Tadokoro T, Hearing VJ, Beer JZ.
    Exp Dermatol. 2013 Apr;22(4):266-71. PMID: 23528212
  • Nrf1 CNC-bZIP protein promotes cell survival and nucleotide excision repair through maintaining glutathione homeostasis.
    Han W, Ming M, Zhao R, Pi J, Wu C, He YY.
    J Biol Chem. 2012 May 25;287(22):18788-95. PMID: 22500024
  • Cyclobutane pyrimidine dimer formation and p53 production in human skin after repeated UV irradiation.
    Yamaguchi Y, Coelho SG, Zmudzka BZ, Takahashi K, Beer JZ, Hearing VJ, Miller SA.
    Exp Dermatol. 2008 Nov;17(11):916-24. PMID: 18363705

References
  • Perturbed gap-filling synthesis in nucleotide excision repair causes histone H2AX phosphorylation in human quiescent cells.
    Matsumoto M, Yaginuma K, Igarashi A, Imura M, Hasegawa M, Iwabuchi K, Date T, Mori T, Ishizaki K, Yamashita K, Inobe M, Matsunaga T.
    J Cell Sci. 2007 Mar 15;120(Pt 6):1104-12. PMID: 17327276
  • In vivo destabilization and functional defects of the xeroderma pigmentosum C protein caused by a pathogenic missense mutation.
    Yasuda G, Nishi R, Watanabe E, Mori T, Iwai S, Orioli D, Stefanini M, Hanaoka F, Sugasawa K.
    Mol Cell Biol. 2007 Oct;27(19):6606-14. PMID: 17682058
  • Neurons and astrocytes exhibit lower activities of global genome nucleotide excision repair than do fibroblasts.
    Yamamoto A, Nakamura Y, Kobayashi N, Iwamoto T, Yoshioka A, Kuniyasu H, Kishimoto T, Mori T.
    DNA Repair (Amst). 2007 May 1;6(5):649-57. PMID: 17236820
  • UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex.
    Sugasawa K, Okuda Y, Saijo M, Nishi R, Matsuda N, Chu G, Mori T, Iwai S, Tanaka K, Tanaka K, Hanaoka F.
    Cell. 2005 May 6;121(3):387-400. PMID: 15882621
  • Trichothiodystrophy fibroblasts are deficient in the repair of ultraviolet-induced cyclobutane pyrimidine dimers and (6-4)photoproducts.
    Nishiwaki Y, Kobayashi N, Imoto K, Iwamoto TA, Yamamoto A, Katsumi S, Shirai T, Sugiura S, Nakamura Y, Sarasin A, Miyagawa S, Mori T.
    J Invest Dermatol. 2004 Feb;122(2):526-32. PMID: 15009740
  • The total amount of DNA damage determines ultraviolet-radiation-induced cytotoxicity after uniformor localized irradiation of human cells.
    Imoto K, Kobayashi N, Katsumi S, Nishiwaki Y, Iwamoto TA, Yamamoto A, Yamashina Y, Shirai T, Miyagawa S, Dohi Y, Sugiura S, Mori T.
    J Invest Dermatol. 2002 Nov;119(5):1177-82. PMID: 12445209
  • DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair.
    Wakasugi M, Kawashima A, Morioka H, Linn S, Sancar A, Mori T, Nikaido O, Matsunaga T.
    J Biol Chem. 2002 Jan 18;277(3):1637-40. Epub 2001 Nov 8. PMID: 11705987
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    Kobayashi N, Katsumi S, Imoto K, Nakagawa A, Miyagawa S, Furumura M, Mori T.
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    Otoshi E, Yagi T, Mori T, Matsunaga T, Nikaido O, Kim ST, Hitomi K, Ikenaga M, Todo T.
    Cancer Res. 2000 Mar 15;60(6):1729-35. PMID: 10749146
  • Three-dimensional visualization of ultraviolet-induced DNA damage and its repair in human cell nuclei.
    Nakagawa A, Kobayashi N, Muramatsu T, Yamashina Y, Shirai T, Hashimoto MW, Ikenaga M, Mori T.
    J Invest Dermatol. 1998 Feb;110(2):143-8. PMID: 9457909
  • Supranuclear melanin caps reduce ultraviolet induced DNA photoproducts in human epidermis.
    Kobayashi N, Nakagawa A, Muramatsu T, Yamashina Y, Shirai T, Hashimoto MW, Ishigaki Y, Ohnishi T, Mori T.
    J Invest Dermatol. 1998 May;110(5):806-10. PMID: 9579550
  • Antigen structural requirements for recognition by a cyclobutane thymine dimer-specific monoclonal antibody.
    Komatsu Y, Tsujino T, Suzuki T, Nikaido O, Ohtsuka E.
    Nucleic Acids Res. 1997 Oct 1;25(19):3889-94. PMID: 9380513
  • High incidence of ultraviolet-B-or chemical-carcinogen-induced skin tumours in mice lacking the xeroderma pigmentosum group A gene.
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    Nature. 1995 Sep 14;377(6545):165-8. PMID: 7675085
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    J Invest Dermatol. 1993 Nov;101(5):685-9. PMID: 8228329
  • DNA damage in UV-irradiated human skin in vivo: automated direct measurement by image analysis (thymine dimers) compared with indirect measurement (unscheduled DNA synthesis) and protection by 5-methoxypsoralen.
    Potten CS, Chadwick CA, Cohen AJ, Nikaido O, Matsunaga T, Schipper NW, Young AR.
    Int J Radiat Biol. 1993 Mar;63(3):313-24. PMID: 8095281
  • Simultaneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6-4)photoproduct from the same mouse immunized with ultraviolet-irradiated DNA.
    Mori T, Nakane M, Hattori T, Matsunaga T, Ihara M, Nikaido O.
    Photochem Photobiol. 1991 Aug;54(2):225-32. PMID: 1780359
  • Wavelength dependent formation of thymine dimers and (6-4) photoproducts in DNA by monochromatic ultraviolet light ranging from 150 to 365 nm.
    Matsunaga T, Hieda K, Nikaido O.
    Photochem Photobiol. 1991 Sep;54(3):403-10. PMID: 1784641

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.