This kit can measure the amount of HIV-1 Gag p24 antigen in cell culture medium handily by a sandwich ELISA (Enzyme Linked Immunosorbent Assay) method.
p24 antigen is a structure protein of HIV-1, so when this is measured, it's possible to presume the virus amount in the sample
This kit is made based on the principle of a sandwich ELISA method using an antibody coated plate as a solid phase, a biotinyl antibody and a peroxidase-labeled streptavidin
・ p24 of subtype AE can be measured at the same sensitivity as subtype B, because affinity purified polyclonal antibody raised against the full length recombinant p24 is used.
・ Risk-free kit, because neither patient sera nor active virus products are used.
・ Assay can be performed at room temperature
Standard curve of HIV-1 p24 measurement by the 2 hour assay.
<Preservation and Expiration Date>
Storage : 2 8°C (Please don't freeze.)
Expiration date for use : 1 year
<Required Reagent, Apparatus and Equipment>
1. Deionized water
2. Test tubes or microtubes (for sample preparation)
3. Micropipettes and tips
4. Microplate reader
1. White, E. L., et al., J. Virol. Methods 70: 113-115 (1998)