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Reagents for Genetic Engineerring

Taq DNA Polymerase (+dNTPs), with Standard buffer

Background

Thermus aquaticus DNA polymerase (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa..

This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.

Application

1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine) at the 3’-blunt ends

*Use of excess amount is not recommended
General composition of PCR reaction mixture (total 50µl)
Taq DNA polymerase (5 units/µl) *0.25 µl
10 x Reaction Buffer (Taq) 5 µl
2.5 mM (each) dNTPs 4 µl
Template <500 ng
Primer 1 0.2 ~ 1.0µM (final conc.)
Primer 2 0.2 ~ 1.0µM (final conc.)
Sterile distilled water up to 50µl


PCR condition
98°C 10sec 25cycles
57°C 30sec 25cycles
72°C 8min 25cycles
(2min in the case of 2kb DNA)

< SDS-PAGE of Taq DNA polymerase >

< Amplification of λ DNA >

Product List

Product Name Cat# Quantity Price

Taq DNA polymerase (+dNTPs), with Standard buffer

BAM-02-001-EX 200UNIT

¥ 5,100
$ 68
€ 51

Taq DNA polymerase Economy (+dNTPs) with Robust buffer

BAM-02-002-5EX 5*200UNIT

¥ 20,400
$ 272
€ 204

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.