Thermus aquaticus DNA polymerase (Taq DNA polymerase) was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and the MW is 94 kDa..
This enzyme is suitable for PCR reactions; capable of amplifying DNA with various primers.
1) High-throughput PCR
2) Colony PCR
3) Incorporation of dUTP, dITP, and fluorescence-labeled nucleotides
4) Primer extension
5) Addition of a single nucleotide (adenosine) at the 3’-blunt ends
|General composition of PCR reaction mixture (total 50µl)|
|Taq DNA polymerase (5 units/µl)||*0.25 µl|
|10 x Reaction Buffer (Taq)||5 µl|
|2.5 mM (each) dNTPs||4 µl|
|Primer 1||0.2 ~ 1.0µM (final conc.)|
|Primer 2||0.2 ~ 1.0µM (final conc.)|
|Sterile distilled water||up to 50µl|
98°C 10sec 25cycles
57°C 30sec 25cycles
72°C 8min 25cycles
(2min in the case of 2kb DNA)
< SDS-PAGE of Taq DNA polymerase >
< Amplification of λ DNA >
Taq DNA polymerase (+dNTPs), with Standard buffer
Taq DNA polymerase Economy (+dNTPs) with Robust buffer
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.