Heterochromatin protein 1 (HP1) is a major component of heterochromatin play a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionarily conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family of proteins is encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1γ is encoded by the Chromobox homolog 3 (CBX3) gene. HP1γhas been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions (Ref 1).
The product is prepared by immunizing rabbit with the synthetic peptide WHSCPEDEAQ-C corresponding to the C-terminal sequence of human HP1γ(Ref 2, 3) and by affinity purification with the peptide. The antiserum preparation has been directed by Prof. T. Haraguchi.
1) Western blotting (1/2000~1/10000)
2) Immunofluoresece staining
3) Chromatin immunoprecipitation (ChIP) (Ref. 3)
Sample:Baby Hamster Kidney cells.
Sample : Baby Hamster Kidney cells
Cells were fixed with para-formaldehyde.
The second antibody : Alexa Fluor 594- conjugated goat anti-rabbit IgG antibody
1. Lomberk G et al. Genome Biol. 7, 228 (2006), review
2. Kametaka A et al. Genes Cells 7, 1231 (2002)
3. Wang F et al. Mol. Cell. Biol. 26, 4028 (2006)
Anti HP1 (GAMMA)/CBX3