PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

Anti Dnmt1 (1037-1386) polyclonal antibod

Background

Dnmt1 (DNA (cytosine-5-)-methyltransferase 1) has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues (epigenetics). Hypermethylation in promoter regions is asssociated with repression of gene expression. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1, 2). This antibody was raised by Prof. S. Tajima of Osaka Univ. (3, 4)

Application

1) Western blotting (0.2~1 ug /ml)
2) Immunoprecipitation Especially good for denatured protein and useful for ChiP assays
3) Indirect immuno-fluorescence staining


Immunodetection of Dnmt1
1. Murine erythrolekemia cells, MEL11A2
2. Mouse myoblast cells,C2C12
3. C2C12 carrying Dnmt1 overexpressing plasmid
4. C2C12 carrying negative control plasmid
1~4; Western blotting.
5~8; Immunoprecipitation of SDS denatured Dnmt1.

<Reference>
This product was used in Ref.3 and 4
1. Di Croce L, et al. (2002). Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor. Science 295: 1079-82.
2. Rhee I, et al. (2002). DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature 416: 552-6.
3. Takagi H, et al (1995) Overexpression of DNA methyltransferase in myoblast cells accelerates myotube formation. Eur. J. Biochem. 282-91
4. Inano K, et al (2000) Maintenance-type DNA methyltransferase is highly expressed in post-mitotic neurons and localized in the cytoplasmic compartment. J. Biochem. 128: 315-21

Product List

Product Name Cat# Quantity Price

Anti Dnmt1 (1037-1386)

BAM-70-203-EX 50UG

¥ 29,000
$ 387
€ 290

Anti Swi6

BAM-63-101-EX 50UL

¥ 30,000
$ 400
€ 300

Anti Cut5/Rad4

BAM-63-107-EX 100UL

¥ 35,000
$ 467
€ 350

Anti DIS3

BAM-63-123EX 100UL

¥ 30,000
$ 400
€ 300

Anti H2B

BAM-63-125-EX 50UL

¥ 30,000
$ 400
€ 300

Anti Pad

BAM-63-133-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Ppa2

BAM-63-135-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Psm1

BAM-63-137-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Sds22

BAM-63-141-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Mrc1

BAM-63-151-EX 50UL

¥ 30,000
$ 400
€ 300

Anti Dnmt1 (1-248)

BAM-70-201-EX 50UG

¥ 26,300
$ 351
€ 263

Anti HP1 (ALPHA) /CBX5

BAM-70-221-EX 50UG

¥ 21,000
$ 280
€ 210

Anti HP1 (BETA)/CBX1

BAM-70-223-EX 50UG

¥ 21,000
$ 280
€ 210

Anti HP1 (GAMMA)/CBX3

BAM-70-225-EX 50UG

¥ 21,000
$ 280
€ 210

Anti 5-Methylcytosine

BAM-51-003-EX 100UG

¥ 34,500
$ 460
€ 345

Anti Rpn3

BAM-62-201-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn5

BAM-62-203-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn7

BAM-62-205-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn9

BAM-62-207-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Rpn12

BAM-62-209EX 50UL

¥ 30,000
$ 400
€ 300

Anti Nob1

BAM-62-211-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Nas6/p28

BAM-62-213-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Tem1

BAM-62-215-EX 100UL

¥ 42,000
$ 560
€ 420