The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nup (Nucleoporin) 153 is a large (153kDa) O-linked glycoprotein which is a component of the basket structure located on the nucleoplasmic face of NPC. Nup153 plays a critical role in nuclear export of RNA and proteins. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by propriety chromatography processes.
1) Western blotting (160 kDa single band in Hela cell extract)
Other applications were not tested
When injected into the nucleus, R4C8 accumulates into the nuclear pores of Hela cells.
Sample is the nuclear membrane fraction of HeLa cells (Left) and NIH3T3 cells (Right).
Left : the nuclear membrane fraction of HeLa cells
Right : NIH3T3 cells
Immunofluorescent : HeLa cells
Iimmuno-fluorescence : HeLa cells
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.