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Anti Cut5/Rad4 (S. pombe) polyclonal antibody

Background

Cut5/Rad4/Dre3 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1, 2). It interacts with chromatin proteins to form the complex required for the initiation and progression DNA synthesis. It contains 4 BRCT domains and the molecular mass is 74.1 kDa with 648 amino acids.

Application

1) Western blotting (500 fold dilution). Not tested for other applications

Figure Identification of the Cut5/Rad4 protein in the crude extract of S. pombe with this antibody
Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins.

Lane M : Size markers (kDa)
Lane 1 : Wild-type cells
Lane 2 : The cut5-5Flag gene replacing the wild-type cut5 gene
Lane 3 : The cut5-13myc gene replacing the wild type gene
Lane 4 : The cut-TAP gene replacing the wild-type gene

* Cut5 protein is known to be sensitive to protease digestion in the C-terminal region and the native and the degradation products are observed as described in Ref.2

<Reference>
1. Saka Y. et al. Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1.Genes Dev.11:3387 (1997)
2. Saka Y. et al. Fission yeast cut5 links nuclear chromatin and M phase regulation in the replication checkpoint control.

Product List

Product Name Cat# Quantity Price

Anti Cut5/Rad4

BAM-63-107-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Swi6

BAM-63-101-EX 50UL

¥ 30,000
$ 400
€ 300

Anti DIS3

BAM-63-123EX 100UL

¥ 30,000
$ 400
€ 300

Anti H2B

BAM-63-125-EX 50UL

¥ 30,000
$ 400
€ 300

Anti Pad

BAM-63-133-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Ppa2

BAM-63-135-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Psm1

BAM-63-137-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Sds22

BAM-63-141-EX 100UL

¥ 35,000
$ 467
€ 350

Anti Mrc1

BAM-63-151-EX 50UL

¥ 30,000
$ 400
€ 300

Anti Dnmt1 (1-248)

BAM-70-201-EX 50UG

¥ 26,300
$ 351
€ 263

Anti Dnmt1 (1037-1386)

BAM-70-203-EX 50UG

¥ 29,000
$ 387
€ 290

Anti HP1 (ALPHA) /CBX5

BAM-70-221-EX 50UG

¥ 21,000
$ 280
€ 210

Anti HP1 (BETA)/CBX1

BAM-70-223-EX 50UG

¥ 21,000
$ 280
€ 210

Anti HP1 (GAMMA)/CBX3

BAM-70-225-EX 50UG

¥ 21,000
$ 280
€ 210

Anti 5-Methylcytosine

BAM-51-003-EX 100UG

¥ 34,500
$ 460
€ 345

Anti Rpn3

BAM-62-201-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn5

BAM-62-203-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn7

BAM-62-205-EX 100UL

¥ 40,000
$ 534
€ 400

Anti Rpn9

BAM-62-207-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Rpn12

BAM-62-209EX 50UL

¥ 30,000
$ 400
€ 300

Anti Nob1

BAM-62-211-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Nas6/p28

BAM-62-213-EX 100UL

¥ 42,000
$ 560
€ 420

Anti Tem1

BAM-62-215-EX 100UL

¥ 42,000
$ 560
€ 420

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.