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Anti-Rad18 (human) polyclonal antibody

Background

The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 as a ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (ref 1~3).

Application

1) Western blotting (1,000 fold dilution)
2) Indirect immuno-fluorescence staining
3) Immuno-precipitation

Fig 1.Indirect immuno-fluorescence staining of Rad18 protein in GM637 cells.
Rad18 protein is stained as yellow dots in nuclei.


Fig 2.Identification of Rad18 protein in crude extract of A549 cells by western blotting.
The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18 protein (ref. 1)

<Reference>
1. Miyase S. et al.J. Biol. Chem. 280, 515-524 (2005)
2. Tateishi S. et al.PNAS 97, 7927-7932 (2000)
3. Watanabe K. et al. EMBO J. 23, 3886-3896 (2004)

Product List

Product Name Cat# Quantity Price

Anti Rad18

BAM-70-023-EX 100UG

¥ 34,500
$ 460
€ 345

References
  • Miyase S.,et al., : Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination. J Biol Chem. 2005 Jan 7;280(1):515-24. Epub 2004 Oct 27. PMID: 15509568
  • Tateiahi S.,et al., : Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens. Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):7927-32. PMID: 10884424
  • Watanabe K. S.,et al., : Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination. EMBO J. 2004 Oct 1;23(19):3886-96. Epub 2004 Sep 9. PMID: 15359278

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