Mrc1 (Mediator of replication checkpoint 1) is a S-phase checkpoint protein of Schizosaccharomyces pombe found at replication forks, required for phosphorylation and activation of Cds1 during DNA replication stress, where it forms a replication-pausing complex, and is phosphorylated by Rad3 and Tel1 kinases.The phosphorylated form of Mrc1 is detected by a change in the electrophoretic mobility.Human homolog of Mrc1 is claspin.
1) Western blotting (x3,000 dilution)
Other applications were not tested.
Western blot analysis of Mrc1 in whole cell extracts.
1. Zhao H. Mol Cell Biol 23: 8395 (2003)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.