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This kit is useful to evaluate osteoclast formation and activation.

Osteoclast Culture Kit

Background

Osteoclast cells derive from bone marrow precursors. Due to their bone resorption activity, osteoclasts are instrumental in the control of bone mass. When cultured appropriately in the presence of M-CSF and RANKL, bone marrow osteoclast precursors differentiate into osteoblasts displaying bone resorption activity.

The Osteoclast Culture Kits contain bone marrow precursors from rat or mouse, and appropriate wash and growth media to insure osteoclast differentiation. Kits including an Osteo Assay Surface Plate - a 96-well plate coated with a manufactured material that supports osteoclast differentiation and is a substrate for osteoclast resorption activity - allow for the convenient and reproducible observation of osteoclast function.


 Rat Osteoclast

Specifications

Description (Cat. No.) Derived From Kit Components
Osteoclast Culture Kit V-4
(PMC-OSC11-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 4 vials
• Wash Medium (100 ml) 1 bottle
• Culture Medium (50 ml) 1 bottle
Osteoclast Culture Kit V-4
(Osteoassay plate)
(PMC-OSC31-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 4 vials
• Wash Medium (100 ml) 1 bottle
• Culture Medium (50 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well)
Osteoclast Culture Kit V-2
(PMC-OSC12-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
Osteoclast Culture Kit V-2
(Osteoassay plate)
(PMC-OSC32-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well) 
Osteoclast Culture Kit V-2
(PMC-OSC13-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
Osteoclast Culture Kit V-2 
(PMC-OSC33-COS)
ICR Mouse Bone Marrow

• Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well)

Osteoclast Culture Kit V-1
(PMC-OSC14-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle 
Osteoclast Culture Kit V-1 
(PMC-OSC34-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle 
• Osteo Assay Surface Plate 1 plate (96 well) 

Osteoclast Culture Kit V-1
(PMC-OSC15-COS)

Human bone marrow • Osteoclast precursor, Human (Frozen, 1.5×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (30 ml) 1 bottle 
Osteoclast Culture Kit V-1
(PMC-OSC35-COS)
Human bone marrow • Osteoclast precursor, Human (Frozen, 1.5×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (30 ml) 1 bottle 
• Osteo Assay Plate 1 plate (96 well) 

Microscope Images

  Phase contrast microscopy of differentiated osteoclasts on the surface of Osteo Assay Plate.
  TRAP staining of Osteoclasts on the surface of Osteo Assay Plate. TRAP staining kit(Cat No. AK04F) was used.
  Pit on the surface of Osteo Assay Plate (Kossa staining)

Product List

Product Name Cat# Quantity Price

Osteoclast Culture Kit V-4

PMC-OSC11-COS 1SET

¥ 152,400
$ 2032
€ 1524

Osteoclast Culture Kit V-2

PMC-OSC12-COS 1SET

¥ 88,800
$ 1184
€ 888

Osteoclast Culture kit V-2 (Osteoassay plate)

PMC-OSC32-COS 1SET

¥ 112,800
$ 1504
€ 1128

Osteoclast Culture Kit V-2

PMC-OSC13-COS 1SET

¥ 238,800
$ 3184
€ 2388

Osteoclast Culture Kit V-2

PMC-OSC33-COS 1SET

¥ 288,000
$ 3840
€ 2880

Osteoclast Culture Kit V-1

PMC-OSC14-COS 1SET

¥ 120,000
$ 1600
€ 1200

Osteoclast Culture Kit V-1

PMC-OSC34-COS 1SET

¥ 156,000
$ 2080
€ 1560

Osteoclast Culture Kit V-1

PMC-OSC15-COS 1SET

¥ 198,000
$ 2640
€ 1980

Osteoclast Culture Kit V-1 (Osteoassay plate)

PMC-OSC35-COS 1SET

¥ 222,000
$ 2960
€ 2220

Osteoclast Culture Medium (for Rat)

PMC-OSCMR-COS 50ML

¥ 36,000
$ 480
€ 360

Osteoclast Culture Medium (for Mouse)

PMC-OSCMM-COS 50ML

¥ 36,000
$ 480
€ 360

Osteoclast Wash Medium

PMC-OSCMW-COS 100ML

¥ 13,200
$ 176
€ 132

RANKL (Human), Recombinant

PMC-AK30-COS 10UG

¥ 21,600
$ 288
€ 216

M-CSF (Human)

PMC-AK39-COS 20UL

¥ 84,500
$ 1127
€ 845

Osteoclast Culture kit V-4 (Osteoassay plate)

PMC-OSC31-COS 1SET

¥ 176,400
$ 2352
€ 1764

References
  • Takeshita et al.(2000) Identification and Characterization of the New Osteoclast Progenitor with acrophage Phenotypes Being Able to Differentiate into Mature Osteoclasts. J. Bone Miner. Res. 15, 1477-1488.
  • Scheven et al. (1998) A sequential culture approach to study osteoclast differentiation from nonadherent porcine bone marrow cells. In Vitro Cell Dev Biol Anim 34, 568?577.
  • Martha et al. (1995) Enhanced Expression of αV Integrin Subunit and Osteopontin during Differentiation of HL-60 Cells along the Monocytic Pathway. Exp. Cell Res. 216, 335-341.
  • Itonaga et al.(1999) 1,25-Dihydroxyvitamin D3 and Prostaglandin E2 Act Directly on Circulating Human Osteoclast Precursors. Biochem. Biophys. Res. Commun. 242, 703-709.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.