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For staining of the Pancreatic beta-cell.

Pancreatic β-cell Fluorescent Staining Kit

Background

Pancreatic β-cell Fluorescent Staining Kit (PMC-AK11F-COS) is designed to stain insulin-producing β-cells, using anti-insulin antibody. This product is available for mouse and rat β-cells.


Mouse islets stained with Pancreatic β-cell Fluorescent Staining Kit

Kit component

ComponentQuantityStorage
Washing Buffer 100 mL 4-10°C
Blocking Buffer 2 mL 4-10°C
Anti-insulin antibody 20 μL 4-10°C
Fluorescein Secondary Antibodies 20 μL 4-10°C

< Protocol >

  1. Transfer pancreatic cells (1.0×105 cells and above) or 10-20 pancreatic islets into a suitable centrifuge tube such as 1.5 mL centrifuge tube.
  2. 2. Centrifuge at 2,000 x g for 3 minutes and remove the supernatant.
  3. Fix cells with 0.5 mL of fixative solution at 4°C over night.
  4. Centrifuge at 10,000 x g for 3 minutes and remove the fixative solution.
  5. Add 1 mL of Washing Buffer to centrifuge tube and centrifuge at 10,000 x g for 3 minutes, and then remove the Washing Buffer. (×3 times)
  6. After removing the Washing Buffer, add 100 μL of Blocking Buffer to centrifuge tube and leave the tube for 30 minutes at room temperature.
  7. Centrifuge at 10,000 x g for 3 minutes and remove the Blocking Buffer.
  8. Add 100 μL of Blocking Buffer to centrifuge tube and then add 2 μL of Anti-insulin antibody to the tube. Mix gently using micropipette and leave the tube at room temperature for one hour.
  9. Centrifuge at 10,000 x g for 3 minutes and remove the Anti-insulin antibody.
  10. Add 1 mL of Washing Buffer to centrifuge tube and centrifuge at 10,000 x g for 3 minutes, and then remove the Washing Buffer. (×3 times)
  11. After removing the Washing Buffer, add 100 μL of Blocking Buffer to centrifuge tube and then add 2 μL of Fluorescein Secondary Antibodies to the tube. Mix gently using micropipette and leave the tube at room temperature for one hour.
  12. Centrifuge at 10,000 x g for 3 minutes and remove the Anti-insulin antibody.
  13. Add 1 mL of Washing Buffer to centrifuge tube and centrifuge at 10,000 x g for 3 minutes, and then remove the Washing Buffer. (×3 times)
  14. Examine cells with an excitation filter of 459 nm and an emission filter of 520 nm.

< Application examples >

Cells isolated from mouse pancreas are stained with Pancreatic β-cell Fluorescent Staining Kit (PMC-AK11F-COS) and observed by fluorescence microscopy or transmitted-light microscopy.

Note

This product is to be used for Research Purposes Only. It is not to be used for Drug or Diagnostic Purposes, nor is it intended for Human Use. Primary cell products may not be resold, modified for resale, or used to manufacture commercial products without the express written consent of Primary Cell Co., Ltd. Except as otherwise expressly set forth in this use manual, Primary Cell does not make any representation or warranties or conditions of any kind, either express or implied, with respect to the products, or information disclosed hereunder, including, but not limited to, the implied warranties of merchantability, fit for a particular purpose, or noninfringement of the intellectual property rights of third parties.

Product List

Product Name Cat# Quantity Price

Pancreatic Beta-cell Staining Kit

PMC-AK11F-COS 1SET

¥ 57,000
$ 760
€ 570

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.