|The Adipocyte Fluorescent Staining Kit (Cat.No.PMC-AK19F-COS) is designed to stain both lipid droplets and nuclei in adipocytes using BODIPY? (Invitrogen Corporation) and H33258, respectively. Furthermore, the amount of lipid per cell and cell shape can be quantified using imaging instrument such as IN Cell Analyzer 1000 (GE Healthcare Company)||
|Tablet for washing||5 tablets||4-10°C|
|Mounting agent||50 mL||4-10°C|
<Protocol: 96-well plate format>
Note: For detection of lipid droplets, examine cells with an excitation filter of 498 nm and an emission filter of 503 nm.
For detection of nucleus, examine cells with an excitation filter of 352 nm and an emission filter of 461 nm.
Visceral preadipocytes collected from a Spraguee Dawley rats (male) were differentiated into mature adipocytes and subjected to staining of lipid droplets (Figure 1) and nuclei (Figure 2).
Figure 1: BODIPY staining of lipid droplets
Figure 2: H33258 staining of nuclei
Adipocyte Fluorescent Staining Kit
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.