Adipocyte accumulates neutral lipid in the cell maturing and neutral lipid is accumulated as lipid droplet. The evaluation of the amount of lipid droplet in the cell becomes criterion of the differentiation of the adipose cell.
Oil red O stain is one of the methods for determination of differentiation in adipocyte. Oil red O, the oilphilic red dye, stains intracellular lipid droplet. This lipid assay kit, consisting of cell fixative and oil red O solution, is used to stain the accumulated oil in adipocyte. Furthermore, this kit is able to quantify amount of oil with organic solvent extraction method.
This kit developed as option kit of White Adipocyte Culture Kit, Brown Adipocyte Culture Kit, Visceral Adipocyte Culture Kit. Put this kit to use oil stain and quantitation in adipocyte.
Human Subcutaneous Adipocyte stained
with Lipid Assay Kit
|Oil red O solution||150 mL×2||4°C|
|Solvent for Oil red O extraction||200 mL×2||4°C|
< Protocol (24-well plate format) >
Viceral adipocytes (PMC-VAC01-COS) were stained by Lipid Assay Kit.
Fig.1 Viceral adipocytes were stained with Oil red O solution
Fig.2 Dye extractions were measured by
spectrophotometer (540 nm)
Lipid Assay Kit
> Tashiro, K., Inamura, M., Kawabata, K., Sakurai, F., Yamanishi, K., Hayakawa, T., Mizuguchi, H. Efficient Adipocyte and Osteoblast Differentiation from Mouse Induced Pluripotent Stem Cells by Adenoviral Transduction. Stem Cells. 27, 1802-1811 (2009)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.