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Latest tool to obesity research

Visceral Adipocyte Culture Kit

Background

Mesenteric adipocytes, a type of visceral adipocytes, are located along the portal vain that transports nutrients absorbed from the intestinal tract to the liver (Fig. 1). Evidence has shown that excess fat accumulation in the visceral adipose tissue contributes to the pathogenesis of Type II diabetes, hypertension and atherosclerosis. The Rat Visceral Adipocyte Culture Kit contains preadipocytes isolated from rat mesentery and culture medium that induces differentiation of precursor cells into mature adipocytes, finally causes hypertrophy. The kit provides a convenient system for studying the mechanism of adipogenesis as well as for screening drugs that prevent metabolic syndrome such as obesity, diabetes and hypertension by blocking the processes of adipogenesis.

Specifications

Description (Cat. No.) Derived From Kit Components
Visceral Adipocyte Culture Kit V-1
(PMC-VAC01-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 3×106 cells) 1 vial
• Visceral Adipocyte Culture Medium Ver.1 (250 mL) 1 bottle
Visceral Adipocyte Culture Kit H-2
(PMC-VACH2-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 1.5×106 cells) 2 vials
• Visceral Adipocyte Culture Medium Ver.1 (250 mL) 1 bottle
Visceral Adipocyte Culture Kit V-1 ver. 2
(PMC-VAC21-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 3×106 cells) 1 vial
• Visceral Adipocyte Culture Medium Ver.2 (250 mL) 1 bottle
Visceral Adipocyte Culture Kit V-1 ver. 2
(PMC-VAC22-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 1.5×106 cells) 2 vials
• Visceral Adipocyte Culture Medium Ver.2 (250 mL) 1 bottle
Visceral Adipocyte Culture Kit PM01
(PMC-VAC31-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 3×106 cells) 1 vial
• Cosmedium VAC SF-V1 (250 mL) 1 bottle
Visceral Adipocyte Culture Kit PM02
(PMC-VAC41-COS)
SD Rat Mesenteric Adipose Tissue • Visceral Predipocyte (Frozen, 3×106 cells) 1 vial
• Cosmedium VAC SF-V2 (250 mL) 1 bottle

Components of Medium:

Visceral Adipocyte Culture Medium ver.1 (Cat.No. PMC-VACMR-COS) is a complete medium designed for optimal culture of visceral preadipocytes in vitro. It is a sterile, liquid basal medium (DE/F-12) which contain essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, inorganic salts, growth factors, hormones, newborn calf serum, and antibiotics.

Visceral Adipocyte Culture Medium ver.2 (Cat.No. PMC-VACM2-COS) which includes 1 ng/mL of insulin and physiological concentration of growth factors is more similar to in vivo condition than ver.1 which includes 10,000 ng/mL of insulin and no growth factors. Both medium do not contain differentiation agent. Cosmedium is suitable for maintenance of preadipocytes.

Product List

Product Name Cat# Quantity Price

Visceral Adipocyte Culture Kit V-1

PMC-VAC01-COS 1SET

¥ 162,000
$ 2160
€ 1620

Visceral Adipocyte Culture Kit H-2

PMC-VACH2-COS 1SET

¥ 162,000
$ 2160
€ 1620

Visceral Adipocyte Culture Medium ver.1

PMC-VACMR-COS 250ML

¥ 31,200
$ 416
€ 312

Visceral Adipocyte Culture Kit V-1 ver.2

PMC-VAC21-COS 1SET

¥ 171,600
$ 2288
€ 1716

Visceral Adipocyte Culture Kit V-1 ver.2

PMC-VAC22-COS 1SET

¥ 171,600
$ 2288
€ 1716

Visceral Adipocyte Culture Medium ver.2

PMC-VACM2-COS 250ML

¥ 42,000
$ 560
€ 420

Visceral Adipocyte Culture kit PM01

PMC-VAC31-COS 1SET

¥ 147,600
$ 1968
€ 1476

Visceral Adipocyte Culture kit PM02

PMC-VAC41-COS 1SET

¥ 157,200
$ 2096
€ 1572

Cosmedium VAC SF-V1

CSR-COS-PM01 250ML

¥ 14,000
$ 187
€ 140

Cosmedium VAC SF-V2

CSR-COS-PM02 250ML

¥ 22,000
$ 294
€ 220

References
  • Mineo H, Oda C, Chiji H, Kawada T, Shimizu K, Taira T. Thiazolidinediones exhibit different effects on preadipocytes isolated from rat mesenteric fat tissue and cell line 3T3-L1 cells derived from mice. Cell Biol Int. 2007 Jul;31(7):703-10. Epub 2007 Jan 14.
  • Shimizu K, Sakai M, Ando M, Chiji H, Kawada T, Mineo H, Taira T. Newly developed primary culture of rat visceral adipocytes and their in vitro characteristics. Cell Biol Int. 2006 Apr;30(4):381-8. Epub 2006 Mar 10.
  • Nagayama, M., Uchida, T., Gohara, K. Temporal and Spatial Variations of Lipid Droplets during Adipocyte Division and Differentiation. J. Lipid Res. 48, 9-18 (2007)
  • Kalgaonkar, S., Nishioka, H., Gross, H. B., Fujii, H. Keen, C. L., Hackman, R. M. Bioactivity of a Flavanol-rich Lychee Fruit Extract in Adipocytes and Its Effects on Oxidant Defense and Indices of Metabolic Syndrome in Animal Models. Phytother. Res. 24, 1223-1228 (2010)
  • Higashi, K., Mikami, T., Yamada, T., Kawashima, H., Kimura, T., Kaneko, H. A Novel Adipokine GM2AP Impairs Insulin Signaling. Biochem. Biophys. Res. Commun. 402, 571-576 (2010)