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Latest tool to obesity research

Brown Adipocyte Culture Kit

Go to "Cell Culture Kits & Cell Culture Assay Kits"

Background

BAT10 is preadipocyte isolated from rat brown adipose tissue. Adipose tissue plays an important role in mammalian energy equilibrium not only as a lipid-dissipating. White adipose tissue mainly has energy-storing function, but brown adipose tissue has very different function as energy-dissipating due to a unique mitochondrial uncoupling protein (UCP).

Brown adipose tissue is especially abundant in newborns and in hibernating mammals. Its primary function is to generate body heat in animals or newborns that do not shiver. In contrast to white adipocyte, which contain a single lipid droplet, brown adipocyte contain numerous smaller droplets and a much higher number of mitochondria, which contain iron and make it brown. Brown fat also contains more capillaries than white fat, since it has a greater need for oxygen than most tissues

Features & Usage

Comparion of UCP-1 gene expression between BAT and 3T3-L1
The UCP-1 gene expression of BAT which accumulated intracellular lipid droplets was examined using RT-PCR. The UCP-1 gene expression of BAT increased when stimulated by 1uM norepinephrine.

 

<UCP-1 gene expression in BAT and 3T3-L1 using RT-PCR>


Experiment example

・ Search for obesity, diabetic, high blood pressure, and drug for arteriosclerosis
・ Development of preventive food of lifestyle-related disease
・ Functional test of antiobestic functional food
・ Lipid metabolic experiment
・ Thermal energy release experiment
・ Function clarification of Brown Adipocyte
・ Screening of new β agonist

Kit Components

Brown Adipocyte Culture Kit D-i (#PMC-BAT10-COS)
Components Size Quantity Storage Conditions
Brown Preadipocytes, Rat
(Adult, 5-8weeks old)
1 x 106cells/vial 1 Liquid Nitrogen
Brown Adipocyte Growth Medium
(Cat. No. PMC-BATGM-COS)
125 ml 1 -20°C Freezer
Brown Adipocyte Differentiation Medium
(Cat. No. PMC-BATDM-COS)
100 ml 1 -20°C Freezer
Brown Adipocyte Maintenance Medium
(Cat. No. PMC-BATMM-COS)
125 ml 1 -20°C Freezer

Brown Adipocyte Culture Kit N-i (#PMC-BAT11-COS)
Components Size Quantity Storage Conditions
Brown Preadipocytes, Rat
(Adult, 5-8weeks old)
1 x 106cells/vial 1 Liquid Nitrogen
Brown Adipocyte Culture Medium
(Cat. No. PMC-BATFM-COS)
250 ml 1 -20°C Freezer

Components of Media:
BATGM, BATDM and BATMM are a complete media designed for optimal culture of rat brown preadipocytes in vitro. These are sterile, liquid basal medium (D-MEM, high glucose) which contain essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, inorganic salts, growth factors, hormones, calf serum, and antibiotics. In addition, BATDM contains insulin and dexamethasone, and BATMM contains insulin. To differentiate preadipocytes to mature adipocytes, use BATDM and BATMM.

Product List

Product Name Cat# Quantity Price

Brown Adipocyte Culture Kit D-i

PMC-BAT10-COS 1SET

¥ 114,000
$ 1520
€ 1140

Brown Adipocyte Culture kit N-i

PMC-BAT11-COS 1SET

¥ 114,000
$ 1520
€ 1140

Brown Adipocyte Differentiation Medium

PMC-BATDM-COS 500ML

¥ 31,200
$ 416
€ 312

Brown Adipocyte Growth Medium

PMC-BATGM-COS 500ML

¥ 31,200
$ 416
€ 312

Brown Adipocyte Maintenance Medium

PMC-BATMM-COS 500ML

¥ 31,200
$ 416
€ 312

Brown Adipocyte Culture Medium

PMC-BATFM-COS 250ML

¥ 31,200
$ 416
€ 312

References

Rehnmark S., Kopecky J., Jacobsson A., Nechad M., Herron D., et al. Brown adipocytes differentated in vitro can express the gene for the uncoupling protein thermogenin effects of hypothyroidism and norepinephrine. Exp. Cell Res., (1989) 182:75-83

G.Ailhaud, P. Grimaldi, and R. Negrel, Cellular and molecular aspects of adipose tissue development. Annu. Rev. Nutr., (1992) 12:207-233

Yasutake Shimizu and Takashi Shimazu, Effects of wortmannin on increased glucose transport by insulin and norepinephrine in primary culture of brown adipocytes. B.B.R.C., (1994) 202 No.2 July 29 660-665

Yasutake Shimizu, Danuta Kielar, Yasuhiko Minokoshi and Takashi Shimazu, Noradrenaline increases glucose transport into brown adipocytes in culture by a mechanism different from that of insulin. Biochem. J., (1996) 314:485-490

Hideki Nikami, Yasutake Shimizu, Michihiro Sumida, Yasuhiko Minokoshi, Toshihide Yosida, Masayuki Saito, and Takashi Shimazu. Expression of β3-adrenoceptor and stimulation of glucose transport by β3-agonists in brown adipocyte primary culture. J. Biochem. (1996) 119:120-125

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.