- Northern and Southern Blot Analysis
This solution requires no additives so handling is easy.
1. Northern Blot Analysis Using PefectHyb
Northern blot analysis was performed with 5 g of total RNA from HeLa cells. Total RNA was extracted using MagExtractor-RNA-(Code No. NPK-201), and then electrophoresis performed in acrylamide gel containing 50% formamide for transfer to the membrane.
An internal probe was prepared from [32P]dCTP with transferrin receptor gene as the template using a Prime-ItII Random Primer Labeling Kit (Code No. SC300385). It was then purified with MagExtractor-PCR & Gel Clean up- (Code No. NPK-601).
Detection was performed with PefectHyb and other commercially available hybridization solutions and by the conventional method to compare results. The results showed that PefectHyb provided higher sensitive detection than other solutions
2. Southern Blot Using PefectHyb
Human genomic DNA VNTR was analyzed.
1 g Genomic DNA extracted from a human cell line and digested with Hae? was electrophoresed and transferred to a membrane. Dig-labeled polymorphic analysis probe YNH24 (D2S44) was used for detection.PefectHyb was confirmed to have higher sensitivity and specificity than other companies’ solutions.