PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

Even staining, high sensitivity detection,low background, high contrast bands

2D-SILVER STAIN-II

Background

For detection of proteins and nucleic acids migration by polyacrylamide gel electrophoresis, silver staining is now being highlighted as a sensitive staining method.
However the original silver staining method is not necessarily easy to work and takes long-time.
2D-SILVER STAIN-II is an improved reagent kit developed to provide simple and fast staining.

Application

2D-SILVER STAIN-II is applicable to the detection of proteins and nucleic acids subjected to polyacrylamide and SDS-polyacrylamide slab gel electrophoresis.


Running Condition
・Loading buffer: Tris SDS
・Sample: PageRulerTM Prestained Protein Ladder #SM1811(Fermentas UAB)
1×4
2×8
3×16
4×32
5×64
6×128
7×256
8×512
9×1024
・Electrode buffer: SDS-Tris-Glycine Electrode buffer
・Running time:30 mA constant current/sheet、About 50 min.
・Dying Method: 2D-Silver Stain

Feature and Advantages

1) Rapid staining results in a short time after electrophoresis.
2) Staining with higher sensitivity
For proteins: 50 to 100 times more sensitive than CBB staining
For nucleic acids: 50 to 100 times more sensitive than EB staining
3) Simple preparation and simple operation.
4) No use of such materials as heavy metals controlled by regulations.
5) Capable of restaining CBB-stained gels. (Double-staining)
6) Staining process can be stopped any time during development for desired chromatic figures.

Spec

Components
Regents Main ingredients Volume
1 Fixing Reagent Thiourea 100mLx1
2 Pretreatment reagent Dithiothreitol, Glutar-aldehyde, Thiourea 100mLx1
3 Staining Solution A Siver nitrate 100mLx1
4 Staining Solution B Ammonium hydroxide, Sodium hydroxide 100mLx1
5 Concentrated developer Citric acid, Folmaldehyde, Sodium hydroxide 100mLx1
6 Stopper Citric acid 100mLx1

References

1) K. Ohsawa et. Al.: Silver Stain for Detecting 10-Femotogram Quantities of protein after Polyacrylamide Gel Electrophoresis, Anal. Biochem., 135, 409(1983)
2) S. Irie: A highly sensitive silver Staining for detection of proteins in polyacrylamide gels, biochemistry(Japan), 52, 411(1980)
3) B.R. Oakley et. al.: Visualization of proteins with silver "stain" for detecting proteins in polyacrylamide gels, Anal. Biochem., 105, 361(1980)
4) H.M. Poehling et. al.: Visualization of proteins with silver "stain", a critical analysis, electrophoresis, 2, 141 (1981)
5) U.K. Laemmli: Cleavage of structural proteins during the assembly of the head of bacteriophage T4., Nature, 227, 680(1970)

Product List

Product Name Cat# Quantity Price

2D-Silver Stain Reagent II [For 10 gels ]

DCB-423413 1PACK

¥ 9,000
$ 120
€ 90

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.