Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
A new biomarker for lipid peroxidation:
# This antibody can be applied to tissue samples from human, rabbit, rat and other animals, because lipid oxidation is species independent. But if you are planning to apply to mouse tissue, blocking of internal mouse IgG will be needed before staining. For example, Vector M.O.M. Immunodetection kit (code.PK2200) may be useful for blocking of mouse IgG.
< Immunohistochemistry >
Recommended concentration: 2μg/ml
Application of anti HEL antibody to HUVECsMaeda R, et.al.:Hypertens Res 31(1)p141-151(2008)
Detection of HEL at Human atherosclerotic lesions. Photo: kindly provided by Dr. Naito
Immunohistochemical procedure PDF
< Western blotting >
Multiple bands will be detected because HEL adducts can be formed in any proteins which have lysine residues.
H. Arai, Y. Kato, K. Fukunaga, S. Mohri, and K. Nakamura: Formation of Nepsilon-(hexanonyl)lysine in oxidized human very-low density lipoprotein. J. Electrophoresis 48, p37-40 (2004)
|Subclass:||Mouse IgG 1kappa|
|Immunogen:||Hexanoyl modified keyhole limpet hemocyanine|
|Specificity:||Cross reactivity is checked for following loxidized lipids:MDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonannal, 2-nonenal, 4-hudroxy-2-nonenal|
|Form:||Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950.100 µg/mL of IgG.|
|Storage:||Store at less than -20℃.|
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.