Antibiotics have been widely used to reduce bacterial infection, and continuos administration of antibiotics show increased survival of the bacteria.This phenomenon is of potential medical significance since tolerance causes failures and facilitates antibiotic resistance. Antibiotics have also been used in stockbreeding and fisheries.The antibiotics remaining in meat and fish are ingested by humans, and development of antibiotic resistance is accelerated. Among various antibiotics, new quinolones have broad antibiotic spectra and a wide variety of derivatives have been developed and used. Contamination of new quinolones causes problems. This kit is simple and easy to use for simultaneous detection of various antibiotics remaining in meat and fish.