The CARD mHTF medium is a novel medium for in vitro fertilization in mice.
It is recommended product by CARD (Center of Animal Resources and Development)
CARD mHTF was specially modified for mice in vitro fertilization.
1. For washing or culturing medium for the in-vitro fertilization
2. As a weight for the sperm cryopreservation
(See Cryopreservation of mouse spermatozoa in CARD FERTIUP)
3. For washing medium of refrigerated epididymis tailpiece etc.
The use of CARD MEDIUM CARD FERTIUP will become more and more comfortably with this CARD mHTF.
We supply 2mL and 5mL of CARD mHTF. 5set (2mL) and 3sets (5mL) are also available!
We recommend that it is used up at once and please do not store an opened ampule for future usage. Please store it at 4 °C before use.
Cryopreservation of Mouse Spermatozoa
(Using CARD FERTIUP Cryoprotectant, CARD mHTF, Sperm Straws, Straw connector and Freezing canister)
In Vitro Fertilization using Cryopreserved Spermatozoa
(Using CARD FERTIUP Preincubation medium, CARD MEDIUM, CARD mHTF and Straw connector)
Collection and Transport at Cold Temperature of Cauda Epididymis(Using Card Cold Transport Kit)
In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
(Using CARD FERTIUP Preincubation Medium: PM and CARD mHTF)
Transport of 2-Cell Embryos at Cold Temperature
(Using CARD Cold Transport Kit, M2 and CARD KSOM)
In vitro fertilization
(Using FERTIUP Preincubation medium, CARD MEDIUM and CARD mHTF)
Simple Vitrification of Mouse Embryos
(Using CARD DAP213 and CARD DMSO)
Embryo Transfer into the Oviduct
Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
(Using CARD FERTIUP Preincubation medium: PM, CARD MEDIUM and CARD mHTF)
Procedure for Warming of Vitrified Embryos
(Using CARD 0.25M sucrose and CARD KSOM)
Storage of Media and Solutions in Ampoules Under Nitrogen Gas
Simple Vitrification of Mouse Oocytes
(Using CARD mHTF)
Tables of Media Composition
CARD 1M DMSO, CARD DAP213, CARD 0.25M Sucrose, CARD mHTF and CARD KSOM
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.