The mHTF medium is a novel medium for in vitro fertilization in mice.
It is recommended product by CARD (Center of Animal Resources and Development)
mHTF was specially modified for mice in vitro fertilization.
1. For washing or culturing medium for the in-vitro fertilization
2. As a weight for the sperm cryopreservation
(See Cryopreservation of mouse spermatozoa in FERTIUP)
3. For washing medium of refrigerated epididymis tailpiece etc.
The use of CARD MEDIUM FERTIUP will become more and more comfortably with this mHTF.
We supply 2mL and 5mL of mHTF. 5set (2mL) and 3sets (5mL) are also available!
We recommend that it is used up at once and please do not store an opened ampule for future usage. Please store it at 4 degrees before use.
Cryopreservation of Mouse Spermatozoa
(Using FERTIUP Cryoprotectant, mHTF, Sperm Straws, Straw connector and Freezing canister)
In Vitro Fertilization using Cryopreserved Spermatozoa
(Using FERTIUP Preincubation medium, CARD MEDIUM, mHTF and Straw connector)
Collection and Transport at Cold Temperature of Cauda Epididymis(Using Card Cold Transport Kit)
In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
(Using FERTIUP Preincubation Medium: PM and mHTF)
Transport of 2-Cell Embryos at Cold Temperature
(Using Card Cold Transport Kit, M2 and KSOM)
In vitro fertilization
(Using FERTIUP Preincubation medium, CARD MEDIUM and mHTF)
Simple Vitrification of Mouse Embryos
(Using DAP213 and DMSO)
Embryo Transfer into the Oviduct
Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
(Using FERTIUP Preincubation medium: PM, CARD MEDIUM and mHTF)
Procedure for Warming of Vitrified Embryos
(Using 0.25M sucrose and KSOM)
Storage of Media and Solutions in Ampoules Under Nitrogen Gas
Simple Vitrification of Mouse Oocytes
Tables of Media Composition
1M DMSO, DAP213, 0.25M Sucrose, mHTF and KSOM
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.