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DNA (cytosine-5) methyltransferase (mouse), Dnmt1

Background

DNA methylation is significant for epigenetic regulation of gene expression, X chromosome inactivation, genomic imprinting, and development. Abberant methylation patterns are associated with certain human tumors and developmental abnormalities. In vertebrates, there are two types of DNA methyltransferase activities; de novo and maintenance types. Two DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation of methylation patterns at an early stage of embryogenesis (de novo-type), while Dnmt1 is responsible for the maintenance of methylation patterns during replication. Dnmt1 favors to methylate the hemimethylated DNA and preferentially methylates one strand of the double-stranded DNA during its processive methylation. This product, mouse Dnmt1 deleting the N-terminal 290 amino acid residues, was expressed using a baculovirus expression system and purified by Prof. S. Tajima and Dr. I. Suetake of Osaka University (ref.2).

Data Link  Swiss-Prot  P13864

Application

1) In vitro metylation of cytosine residues in hemimethylated DNA at 5’….CG…3’. (ref. 1,2)
2) Antigen for anti-mammalian Dnmt1 antibodies.


SDS-polyacrylamide gel electrophoresis of recombinant Dnmt1.

Form : 0.5mg protein/ml in 0.2M NaCl, 10mM HEPES (pH 7.4), 50% glycerol

Definition of specific activity : 1 unit is defined as the amount of the enzyme that transfer 1 pmole of methyl group to poly dI-dC substrate during 30 minutes at 37℃
Specific activity : 17 units/ul
Quality Assurance : Greater than 95% protein determined by SDS-PAGE (CBB staining) (Fig.)

<References>
1. Vilkaitis G. et al. (2005) “Processive methylation of hemi- methylated CpG sites by mouse Dnmt1 DNA methyl-transferase.” J. Biol. Chem. 280: 64-72 PMID:15509558
2.Tajima S and Suetake I (1998) “Regulation and function of DNA methylation in vertebrates.”J Biochem.123:993-999 Review PMID : 9603984

Product List

Product Name Cat# Quantity Price

DNA (cytosine-5) methyltransferase

BAM-10-201-EX 300UNIT

¥ 33,000
$ 440
€ 330

Anti Dnmt1 (1037-1386)

BAM-70-203-EX 50UG

¥ 29,000
$ 387
€ 290

Anti Dnmt3b

BAM-70-206-EX 100UG

¥ 35,000
$ 467
€ 350

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.