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PNIPAAm-PEG 3D Thermoreversible Hydrogel Cell Culture

Mebiol® Gel Hydrogel Literature Citations and References

Cell Culture and Tissue Regeneration

Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation)
Defined and Scalable Differentiation of Human Oligodendrocyte Precursors from Pluripotent Stem Cells in a 3D Culture System.
Rodrigues GMC, Gaj T, Adil MM, Wahba J, Rao AT, Lorbeer FK, Kulkarni RU, Diogo MM, Cabral JMS, Miller EW, Hockemeyer D, Schaffer DV.
Stem Cell Reports. 2017 Jun 6;8(6):1770-1783. PMID: 28552605
Review
Engineering-derived approaches for iPSC preparation, expansion, differentiation and applications.
Li Y, Li L, Chen ZN, Gao G, Yao R, Sun W.
Biofabrication. 2017 Jul 31;9(3):032001. PMID: 28759433
Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation)
An Integrated Miniature Bioprocessing for Personalized Human Induced Pluripotent Stem Cell Expansion and Differentiation into Neural Stem Cells.
Haishuang Lin, Qiang Li, Yuguo Lei
Sci Rep. 2017 Jan 6;7:40191. doi: 10.1038/srep40191. PMID: 28057917
Adoptive transfer of T cells into recipient mice
Obesity accelerates T cell senescence in murine visceral adipose tissue.
Shirakawa K, Yan X, Shinmura K, Endo J, Kataoka M, Katsumata Y, Yamamoto T, Anzai A, Isobe S, Yoshida N, Itoh H, Manabe I, Sekai M, Hamazaki Y, Fukuda K, Minato N, Sano M.
J Clin Invest. 2016 Dec 1;126(12):4626-4639. PMID: 27820698
Review
Stem Cell Organoid Engineering
Xiaolei Yin, Benjamin E. Mead, Helia Safaee, Robert Langer, Jeffrey M. Karp and Oren Levy
Cell Stem Cell. 2016 Jan 7; 18(1): 25?38. PMID: 26748754
Affordable glioblastoma TICs for Drug discovery
Scalable Production of Glioblastoma Tumor-initiating Cells in 3 Dimension Thermoreversible Hydrogels.
Qiang Li, Haishuang Lin, Ou Wang, Xuefeng Qiu, Srivatsan Kidambi, Loic P. Deleyrolle, Brent A. Reynolds & Yuguo Lei.
Scientific Reports 6, Article number: 31915 (2016) PMID: 27549983
Biological processes
Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.
Xiao Huang, Qirui Hu, Yifan Lai, Demosthenes P. Morales, Dennis O. Clegg and Norbert O. Reich
DOI: 10.1002/adma.201603318 PMID: 27787919
Osteopontin Production
A CD153+CD4+ T Follicular Cell Population with Cell-Senescence Features Plays a Crucial Role in Lupus Pathogenesis via Osteopontin Production
Suhail Tahir, Yuji Fukushima, Keiko Sakamoto, Kyosuke Sato, Harumi Fujita, Joe Inoue, Toshimitsu Uede, Yoko Hamazaki, Masakazu Hattori, and Nagahiro Minato
J. Immunol., Jun 2015; 194: 5725 - 5735. PMID: 25972477
Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation)
Developing Defined and Scalable 3D Culture Systems for Culturing Human Pluripotent Stem Cells at High Densities.
Yuguo Lei, Daeun Jeong, Jifang Xiao, David V. Schaffer
Cell Mol Bioeng. 2014 Jun;7(2):172-183. PMID: 25419247
Review
Scalable culture of human pluripotent stemcells in 3D
Todd C. McDevitt
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):20852-3. PMID: 24327732
Stem Cells (corneal limbal), review
Towards the use of hydrogels in the treatment of limbal stem cell deficiency.
Bernice Wright, Shengli Mi, and Che J. Connon
Drug Discov Today. 2013 Jan; 18(1-2): 79-86. PMID: 22846850
Stem Cells (human pluipotent stem cell (hPSC) line expansion and differentiation)
A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation.
Yuguo Leia, David V. Schaffer
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):E5039-48. PMID: 24248365
Cell Culture (kidney cyst formation)
Mxi1 influences cyst formation in three-dimensional cell culture.
Yook YJ, Yoo KH, Song SA, Seo MJ, Ko JY, Kim BH, Lee EJ, Chang E, Woo YM, Park JH
BMB Rep. 2012 Mar;45(3):189-93. PMID: 22449707
ROS Cell-Based Assay
Effect of green tea extract on reactive oxygen species produced by neutrophils from cancer patients.
Katsuhiko Suzuki, Satoshi Ohno, Yoko Suzuki, Yumiko Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamoto, Ken-O Ishihara, Tsutomu Nozaki, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori
Anticancer Res. 2012 Jun;32(6):2369-75. PMID: 22641677
ROS Cell-Based Assay
Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils.
Suzuki Y, Ohno S, Okuyama R, Aruga A, Yamamoto M, Miura S, Yoshioka H, Mori Y, Suzuki K
Anticancer Res. 2012 Feb;32(2):565-70 PMID: 22287746
Cell Culture for drug screening
In vitro Thermoreversible Gel Disc Quantitative Assay of Rat Angiogenesis.
Shin Wakui, Tomoko Muto, Naohiko Anzai, Hiroyuki Takahashi, Promsuk Jutabha, Akihiko Sakata, Michael F. Wempe, Hiroshi Hano, Hitoshi Endou
AATEX 16(2), 59-65, 2011 PMID:
Wound healing
Wound dressing of newly developed thermobelling thermoreversible hydrogel.
H. Yoshioka, Y. Mori, S. Kubota
Jinko Zoki Vol. 27 (1998) No. 2 P 503-506 PMID:
Organ Culture
FGF signaling directs a center-to-pole expansion of tubulogenesis in mouse testis differentiation.
Hiramatsu R, Harikae K, Tsunekawa N, Kurohmaru M, Matsuo I, Kanai Y
Development. 2010 Jan;137(2):303-12. PMID: 20040496
Stem Cell Culture, Regenerative Medicine
Application of a Thermo-Reversible Gelation Polymer, Mebiol Gel, for Stem Cell Culture and Regenerative Medicine.
Kataoka K, Huh N
Journal of Stem Cell & Regenerative Medicine 2010 Vol. 6 (1): p10-14 (2010) PMID: 24693055
Embryo Explant Culture
Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo.
Yamamoto M, Beppu H, Takaoka K, Meno C, Li E, Miyazono K, Hamada H
J Cell Biol. 2009 Jan 26;184(2):323-34 PMID: 19153222
Virus infection/replication system
3D cultured immortalized human hepatocytes useful to develop drugs for blood-borne HCV.
Aly HH, Shimotohno K, Hijikata M
Biochem Biophys Res Commun. 2009 Feb 6;379(2):330-4. PMID: 19103167
Stem Cells (corneal limbal)
Limbal stem cells: application in ocular biomedicine.
Vemuganti GK, Fatima A, Madhira SL, Basti S, Sangwan VS
Int Rev Cell Mol Biol. 2009;275:133-81. PMID: 19491055
Stem Cells (corneal limbal)
Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: an animal model.
Sitalakshmi G, Sudha B, Madhavan HN, Vinay S, Krishnakumar S, Mori Y, Yoshioka H, Abraham S.
Tissue Eng Part A. 2009 Feb;15(2):407-15. PMID: 18724830
Stem Cells (mesenchymal)
Chondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemically synthesized thermoreversible polymer.
I. Kao, C. Yao, Y. Chang, T. Hsieh, S. Hwang,
Chin J Physiol. 2008 Aug 31;51(4):252-8 PMID: 19112883
Hepatocyte Transplantation
Intraperitoneal Transplantation of Hepatocytes Embedded in Thermoreversible Gelation Polymer (Mebiol Gel) in Acute Liver Failure Rat Model.
N. Parveen, A.A. Khan, S. Baskar, M.A. Habeeb, P. Ravindra Babu, A. Samuel, Y. Hiroshi, M. Yuichi, C.M. Habibullah
Hepatitis Monthly, Volume 275 8, Issue 4, Autumn, November 2008 Page S71 PMID:
Stem Cells (Corneal Limbal)
A Rabbit Model of Ex Vivo Cultivation and Transplantation of Autologous Limbal Epithelial Cells Grown in a Thermo-reversible Gelation Polymer (Mebiol Gel).
Sitalakshmi G, Sudha B, Vinay S, Madhavan H, Krishnakumar S, Mori Y, Yoshioka H, Abraham S
J Stem Cells Regen Med. 2006 Dec 26;1(1):44-5. eCollection 2006. PMID: 24692863
Virus Reproduction and Drug Screening
Production of infectious hepatitis C virus particles in three -dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b.
K. Murakami, K. Ishii, Y. Ishihara, S. Yoshizaki, K. Tanaka. Y. Gotoh, H. Aizaki, M. Kohara, H. Yoshioka, Y. Mori, N. Manabe, I., Shoji, T. Sata, R. Bartenschlarger, Y. Matsuura, T. Miyamura, T. Suzuki,
Virology. 2006 Aug 1;351(2):381-92. Epub 2006 May 6 PMID: 16678876
Tissue Regeneration (Liver)
Thermoreversible gelation polymer induces the emergence of hepatic stem cells in the partially injured rat liver.
Nagaya, M., Kubota, S., Suzuki, N., Akashi, K., Mitaka, T.,
Hepatology. 2006 May;43(5):1053-62. PMID: 16628635
Stem Cells (epithelial)
Isolation of epithelial stem cells from dermis by a three-dimensional culture system.
Reinhold J. Medina, Ken Kataoka, Mikiro Takaishi, Masahiro Miyazaki, Nam-ho Huh,
J Cell Biochem. 2006 May 1;98(1):174-84. PMID: 16408300
Tissue Engineering (Bone)
In Vitro Culture of Chondrocytes in a Novel Thermoreversible Gelation Polymer Scaffold Containing Growth Factors.
Yasuda, K. Kojima, K. W. Tinsley, H. Yoshioka, Y. Mori, C. A. Vacanti,
Tissue Eng. 2006 May;12(5):1237-45. PMID: 16771637
Stem Cells (corneal limbal)
Cultivation of human corneal limbal stem cells in Mebiol Gel A thermoreversible gelation polymer.
B. Sudha, H. N. Madhavan, G. Sitalakshmi, J. Malathi, S. Krishnakumar, Y. Mori, H. Yoshioka, S. Abraham,
Indian J Med Res. 2006 Dec;124(6):655-64. PMID: 17287553
Hepatocyte Culture
Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes.
Aly HH, Watashi K, Hijikata M, Kaneko H, Takada Y, Egawa H, Uemoto S, Shimotohno K
J Hepatol. 2007 Jan;46(1):26-36. Epub 2006 Oct 30. PMID: 17112629
Embryo Culture
Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm.
Kimura-Yoshida C, Nakano H, Okamura D, Nakao K, Yonemura S, Belo JA, Aizawa S, Matsui Y, Matsuo I.
Dev Cell. 2005 Nov;9(5):639-50. PMID: 16256739
Liver Regeneration
Evaluation of thermoreversible gelation polymer for regeneration of focal liver injury.
M. Nagaya, S. Kubota, N. Suzuki, M. Tadokoro, K. Akashi,
Eur Surg Res. 2004 Mar-Apr;36(2):95-103. PMID: 15007262
Stem Cell Culture and Differentiation
Gene expression profile of human mesenchymal stem cells during osteogenesis in three-dimensional thermoreversible gelation polymer.
Hishikawa, K., Miura, S., Marumo, T., Yoshioka, H., Mori, Y., Takato, T., Fujita, T.,
Biochem Biophys Res Commun. 2004 May 14;317(4):1103-7. PMID: 15094382
Evalulation of Cell Line Growth in Mebiol Gel
A study on the growth of continuous culture cell lines embedded in Mebiol Gel.
H. N. Madhavan, J. Malathi, Patricia, Rinku Joseph, Yuichi Mori, Samuel JK Abraham and Hiroshi Yoshioka,
CURRENT SCIENCE 87(9), 1275-1277 (2004) PMID:
Spheroid Culture (Cancer)
A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer.
Tsukikawa, S., Matsuoka, H., Kurahashi, Y., Konno, Y., Satoh, K., Satoh, R., Isogai, A., Kimura, K., Watanabe, Y., Nakano, S., Hayashi, J., Kubota, S.,
Artif Organs. 2003 Jul;27(7):598-604. PMID: 12823414
Islet Transplantation
In vitro studies on a new method for islet microencapsulation using a thermoreversible gelation polymer, N-isopropylacrylamide-based copolymer.
Shimizu, S., Yamazaki , M., Kubota, S., T. Ozasa, H. Moriya, Kobayashi, K., Mikami, M., Mori, Y., and Yamaguchi, S.,
Artif Organs. 1996 Nov;20(11):1232-7. PMID: 8908335

Non-Cell Culture Applications

Protein Crystallization Scaffold
Viscous hydrophilic injection matrices for serial crystallography
Gabriela Kovacsova, Marie Luise Grunbein, Marco Kloos, Thomas R. M. Barends, Ramona Schlesinger, Joachim Heberle, Wolfgang Kabsch, Robert L. Shoeman, R. Bruce Doak and Ilme Schlichting
IUCrJ Volume 4, Part 4, July 2017, Pages 400-410 PMID:
Protein Crystallization Scaffold
A Novel Approach for Protein Crystallization by a Synthetic Hydrogel with Thermoreversible Gelation Polymer.
Shigeru Sugiyama, Noriko Shimizu, Gen Sazaki, Mika Hirose, Yoshinori Takahashi, Mihoko Maruyama, Hiroyoshi Matsumura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori
Cryst. Growth Des., 2013, 13 (5), pp 1899-1904 PMID:
DNA molecular Sorting
Microfluidic active sorting of DNA molecules labeled with single quantum dots using flow switching by a hydrogel sol?gel transition.
Mai Haneoka, Yoshitaka Shirasaki, Hirokazu Sugino, Tokihiko Aoki, Takahiro Arakawa, Kazuto Ozaki, Dong Hyun Yoon, Noriyuki Ishii, Ryo Iizuka, Shuichi Shoji, Takashi Funatsu
Chemical, Volume 159, Issue 1, 28 November 2011, Pages 314-320 PMID:
Drug delivery
Novel local drug delivery system using thermoreversible gel in combination with polymeric microspheres or liposomes.
Arai T, Benny O, Joki T, Menon LG, Machluf M, Abe T, Carroll RS, Black PM.
Anticancer Res. 2010 Apr;30(4):1057-64. PMID: 20530409
Cell sorting switch
Microfluidic cell sorter with flow switching triggered by a sol?gel transition of a thermo-reversible gelation polymer.
Kazuto Ozaki, Hirokazu Sugino, Yoshitaka Shirasaki, Tokihiko Aoki, Takahiro Arakawa, Takashi Funatsu, Shuichi Shoji
Chemical, Volume 150, Issue 1, 21 September 2010, Pages 449-455 PMID:
Cell sorting switch
On-Chip Cell Sorting System Using Thermoreversible Gelation Polymer.
Yoshitaka Shirasaki, Hirokazu Sugino, Masayasu Tatsuoka, Jun Mizuno, Shuichi Shoji, and Takashi Funatsu
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, VOL. 13, NO. 2, MARCH/APRIL 2007 PMID:
Drug delivery
Novel drug delivery system using thermoreversible gelation polymer for malignant glioma.
T. Arai, T. Joki, M. Akiyama, M. Agawa, Y. Mori, H. Yoshioka, T. Abe,
J Neurooncol. 2006 Mar;77(1):9-15. PMID: 16292493
Cell sorting switch
On-Chip Cell Sorting System Using Laser-Induced Heating of a Thermoreversible Gelation Polymer to Control Flow.
Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita, T. Funatsu,
Anal Chem. 2006 Feb 1;78(3):695-701. PMID: 16448041
Scaffold for DNA Electrophoresis and Recovery
Separation and recovery of DNA fragments by electrophoresis through a thermoreversible hydrogel composed of poly(ethylene oxide) and poly(propylene oxide).
Yoshioka, H., Mori, Y. and Shimizu, M.,
Anal Biochem. 2003 Dec 15;323(2):218-23. PMID: 14656528

Physical Properties

Bio rapid prototyping by extruding/aspirating/refilling thermoreversible hydrogel.
Iwami K, Noda T, Ishida K, Morishima K, Nakamura M, Umeda N.
Biofabrication PMID: 20811123
Endovascular treatment of experimental aneurysms using a combination of thermoreversible gelation polymer and protection devices: feasibility study.
H. Takao, Y. Murayama, T. Saguchi, T. Ishibashi, M. Ebara, K. Irie, H. Yoshioka,Y. Mori, S. Ohtsubo, F. Vinnuela, T. Abe
Neurosurgery. 2009 Sep;65(3):601-9; discussion 609. PMID: 19687707
Endovascular treatment of experimental cerebral aneurysms using thermoreversible liquid embolic agents.
Takao H, Murayama Y, Saguchi T, Ishibashi T, Ebara M, Irie K, Yoshioka H, Mori Y, Ohtsubo S, Vinuela F, Abe T.
Interv Neuroradiol. 2006 Jan 20 PMID: 20569622
Thermoreversible gelation on heating and on cooling of an aqueous gelatin-poly(N-isopropylacrylamide) conjugate.
Yoshioka, H., Mori, Y., Tsukikawa, S. and Kubota, S.,
Polym. Adv. Tech., 9, 155-158 (1998) PMID:
A synthetic hydrogel with thermoreversible gelation. III. : an NMR study of the sol-gel transition.
Yoshioka, H., Cushman, J.A., Mori, Y. and Tsuchida, E.,
Polym. Adv. Tech., 5, 122-127 (1994) PMID:
A synthetic hydrogel with thermoreversible gelation. II. : Effect of added salts.
Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E.,
J. Macromol. Sci., A31(1), 121-125 (1994) PMID:
A synthetic hydrogel with thermoreversible gelation. I. : Preparation and rheological properties.
Yoshioka, H., Mikami, M., Mori, Y. and Tsuchida, E.,
J. Macromol. Sci., A31(1), 113-120 (1994) PMID:

Product List

Product Name Cat# Quantity Price

Mebiol Gel

MBG-PMW20-1001 1*10ML

¥ 30,000
$ 400
€ 300

Mebiol Gel

MBG-PMW20-5001 1*50ML

¥ 60,000
$ 800
€ 600

Mebiol Gel

MBG-PMW20-1005 5*10ML

¥ 100,000
$ 1334
€ 1000

Mebiol Gel

MBG-PMW20-5005 5*50ML

¥ 200,000
$ 2667
€ 2000

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.