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Reagents to stabilise and improve spermatozoa cryopreservation results and in vitro fertilization (IVF) rates for laboratory mice

FERTIUP® Cryoprotectant
FERTIUP® Preincubation Medium
CARD MEDIUM® Mouse Fertilization Medium
FERTIUP® PM -CARD MEDIUM® set

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Background

Sperm cryopreservation is a practical and cost-effective method of archiving mouse strains in the laboratory. FERTIUP® and CARD MEDIUM® are high-performance media for mouse sperm cryopreservation, sperm preincubation and in vitro fertilization (IVF).

When used in combination, FERTIUP® and CARD MEDIUM® provide a high and stable fertilization rate using frozen-thawed mouse sperm. This will allow you to reproduce mice whenever you need them.

The FERTIUP® sperm cryoprotectant and sperm preincubation medium greatly improve fertilization rates of frozen-thawed sperm in C57BL/6 and 129 Mice strains, from less than 10% (using conventional methods) to more than 30%.

In addition, our new CARD MEDIUM® enhances the fertility rate to over 80%.

It goes without saying that FERTIUP® and CARD MEDIUM® have no reproduction toxicity of any kind.

Our newly developed system for sperm cryopreservation and IVF using FERTIUP® and CARD MEDIUM® provides superior support for the safe and reliable preservation of your valuable mouse strains.


Fig. 1: Offspring derived via in vitro fertilization using cryopreserved sperm treated with FERTIUP® and CARD MEDIUM®.


What is FERTIUP® and CARD MEDIUM®?

FERTIUP® and CARD MEDIUM® are reagents to stabilise and improve spermatozoa cryopreservation results and in vitro fertilization rates (IVF) for laboratory mice. The series of products consits of the following:

  1. FERTIUP® Cryoprotectant (KYD-001-EX)  →Reagent used to freeze sperm for storage.
  2. FERTIUP® Preincubation Medium (KYD-002-EX)  →Reagent used to pre-incubate sperm after thawing from frozen state. To perform before IVF.
  3. CARD MEDIUM® Mouse Fertilization Medium (KYD-003-EX)  →Medium used for IVF
Usage of FERTIUP® alone will lead to higher fertilization rates, but combined usage with CARD MEDIUM® will lead to even higher fertilization rates and success.

Feature and Advantages 

  •  High fertilization rate (over 80%)
  •  Improvements in the management of genetically modified mice (Tg/KO)
  •  Reduction in labor, facilities, and breeding cost.
  •  Reduction in time required to expand a mouse colony
  •  Efficient production of strains which are notoriously difficult to breed


Fig. 2: Fertilization rates using frozen-thawed sperm with FERTIUP® and with FERTIUP® and CARD MEDIUM®



Fig. 3: Fertilizaion rates using FERTIUP® Preincubation Medium and CARD MEDIUM® for fresh and frozen sperm in various strains.


Manual and Frequently Asked Questions (FAQ)

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FERTIUP®


We offer a variety of sizes available for your convenient usage (0.5mL, 1mL, 5*0.5mL, 5*1mL).
Please note once the ampule is opened, we recommend you to use it up, and not store an opened ampule for future usage.

CARD MEDIUM®


[Kit Components]
An ampoule including medium (A): 3 mL
A vial including powder (B): 30 mg
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22μm)

Set Products (FERTIUP® + CARD MEDIUM®)

 

FERTIUP® PM 1ML-CARD MEDIUM® set
(KYD-004-EX)

[Kit Components]
An ampoule including medium (A): 3 mL
A vial including powder (B): 30 mg
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22μm)
An ampule of PM (1.0mL) 

FERTIUP® PM 0.5ML-CARD MEDIUM® set
(KYD-005-EX)

[Kit Components]
An ampoule including medium (A): 3 mL
A vial including powder (B): 30 mg
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22μm) 
An ampule of PM (0.5mL) 

Product List

Product Name Cat# Quantity Price

FERTIUP Cryoprotectant: CPA

KYD-001-EX 1ML

¥ 6,500
$ 87
€ 65

FERTIUP Cryoprotectant: CPA

KYD-001-EX-X5 5*1ML

¥ 30,000
$ 400
€ 300

FERTIUP Cryoprotectant: CPA

KYD-001-05-EX 0.5ML

¥ 3,800
$ 51
€ 38

FERTIUP Cryoprotectant: CPA

KYD-001-05-EX-X5 5*0.5ML

¥ 17,500
$ 234
€ 175

FERTIUP Preincubation Medium: PM

KYD-002-EX 1ML

¥ 6,500
$ 87
€ 65

FERTIUP Preincubation Medium: PM

KYD-002-EX-X5 5*1ML

¥ 30,000
$ 400
€ 300

FERTIUP Preincubation Medium: PM

KYD-002-05-EX 0.5ML

¥ 3,800
$ 51
€ 38

FERTIUP Preincubation Medium: PM

KYD-002-05-EX-X5 5*0.5ML

¥ 17,500
$ 234
€ 175

CARD MEDIUM

KYD-003-EX 1KIT

¥ 12,000
$ 160
€ 120

FERTIUP PM 1ML-CARD MEDIUM set

KYD-004-EX 1SET

¥ 16,000
$ 214
€ 160

FERTIUP PM 0.5ML-CARD MEDIUM set

KYD-005-EX 1SET

¥ 14,000
$ 187
€ 140

Citation
  • Long-term melatonin treatment delays ovarian aging.
    Tamura H, Kawamoto M, Sato S, Tamura I, Maekawa R, Taketani T, Aasada H, Takaki E, Nakai A, Reiter RJ, Sugino N.
    J Pineal Res. 2017 Mar;62(2). PMID: 27889913
  • Mild reproductive impact of a Y chromosome deletion on a C57BL/6J substrain.
    MacBride MM, Navis A, Dasari A, Perez AV.
    Mamm Genome. 2017 Jun;28(5-6):155-165. PMID: 28283737
  • Identification of an anti-sperm auto-monoclonal antibody (Ts4)-recognized molecule in the mouse sperm acrosomal region and its inhibitory effect on fertilization in vitro.
    Yoshitake H, Oda R, Yanagida M, Kawasaki Y, Sakuraba M, Takamori K, Hasegawa A, Fujiwara H, Araki Y.
    J Reprod Immunol. 2016 Jun;115:6-13. PMID: 27064211
  • Severe damage to the placental fetal capillary network causes mid- to late fetal lethality and reduction in placental size in Peg11/Rtl1 KO mice.
    Kitazawa M, Tamura M, Kaneko-Ishino T, Ishino F.
    Genes Cells. 2017 Feb;22(2):174-188. PMID: 28111885

References
  • Cryopreservation of mouse spermatozoa and in vitro fertilization.
    Nakagata N.
    Methods Mol Biol. 2011;693:57-73. PMID: 21080274
  • Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm.
    Takeo T, Nakagata N.
    Lab Anim. 2010 Apr;44(2):132-7. PMID: 20357317
  • Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm.
    Takeo T, Kondo T, Haruguchi Y, Fukumoto K, Nakagawa Y, Takeshita Y, Nakamuta Y, Tsuchiyama S, Shimizu N, Hasegawa T, Goto M, Miyachi H, Anzai M, Fujikawa R, Nomaru K, Kaneko T, Itagaki Y, Nakagata N.
    J Am Assoc Lab Anim Sci. 2010 Jul;49(4):415-9. PMID: 20819385
  • Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature.
    Takeo T, Kaneko T, Haruguchi Y, Fukumoto K, Machida H, Koga M, Nakagawa Y, Takeshita Y, Matsuguma T, Tsuchiyama S, Shimizu N, Hasegawa T, Goto M, Miyachi H, Anzai M, Nakatsukasa E, Nomaru K, Nakagata N.
    Cryobiology. 2009 Apr;58(2):196-202. PMID: 19168045
  • Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells.
    Takeo T, Hoshii T, Kondo Y, Toyodome H, Arima H, Yamamura K, Irie T, Nakagata N.
    Biol Reprod. 2008 Mar;78(3):546-51. PMID: 18046011
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.