Epitope tagging has become a powerful tool for detection and purification of expressed proteins. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This Anti-DYKDDDDK monoclonal antibody is a very high sensitivity antibody in not only Western blot (WB), Immunoprecipitation (IP), and Immunocytochemistry (ICC) but also Flow Cytometry (FCM).
|Not Tested||0.1-1.0||Not Tested||0.1-1.0|
Note : Recognition site is N-term Flag only
Fig. 1 ICC Sample: DYKDDDDK_mBLT1 transfected CHO-K1
Anti DYKDDDDK MAb (2H8) 1μg/ml
Sigma antibody A 1μg/ml
Sigma antibody B 1μg/ml
This cell staining is done in CHO cell by the DYKDDDDK addition to N-terminus of prostaglandin dehydrogenase. (antibody concentration : 1μg/ml)
< Flow Cytometry (FCM) >
Fig.2 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 1μg/ml : 2H8, M2, M5 ) and anti-Alexa488-mouse IgG
Fig.3 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 1μg/ml : 2H8, M5 ) and anti-Alexa488-mouse IgG
Fig.4 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 0.3 μg/ml , 0.14 μg/ml, 0.04 μg/ml, 0.03 μg/ml:2H8, M5 ) and anti-Alexa488-mouse IgG
< Immunoprecipitation >
Fig.5 Immunoprecipitation analysis of extracts from CHO cells with anti-FLAG antibody
(1μg/ml, 0.5 μg/ml, 0.1 μg/ml : 2H8, M2, M5 )
|Immunogen||DYKDDDDK peptide expressing cell line|
|Raised in||Mouse (BALB/c)|
|Buffer||PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]|
|Storage||Store below 20℃. Once thawed, store at 4℃. Repeated freeze-thaw cycles should be avoided.|
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.