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High sensitivity DYKDDDDK Tag antibody for analysis with WB, IP, ICC and FCM

Anti DYKDDDDK Monoclonal Antibody (Clone No. 2H8)

Background

Epitope tagging has become a powerful tool for detection and purification of expressed proteins. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.

This Anti-DYKDDDDK monoclonal antibody is a very high sensitivity antibody in not only Western blot (WB), Immunoprecipitation (IP), and Immunocytochemistry (ICC) but also Flow Cytometry (FCM).

Application

Recommended Antibody Dilutions
ElISA WB IHC ICC
Not Tested 0.1-1.0 Not Tested 0.1-1.0
IP FCM IF Neutralization
0.1-1.0 0.1-1.0 1.0-5.0 -

(μg/mL)

Note : Recognition site is N-term Flag only


Fig. 1 ICC Sample:  DYKDDDDK_mBLT1 transfected CHO-K1
Anti DYKDDDDK MAb (2H8)      1μg/ml
Sigma antibody A                1μg/ml
Sigma antibody B                1μg/ml

This cell staining is done in CHO cell by the DYKDDDDK addition to N-terminus of prostaglandin dehydrogenase. (antibody concentration : 1μg/ml)

< Flow Cytometry (FCM) >

Fig.2 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 1μg/ml : 2H8, M2, M5 ) and anti-Alexa488-mouse IgG


Fig.3 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 1μg/ml : 2H8, M5 ) and anti-Alexa488-mouse IgG


Fig.4 Flow cytometory analysis of CHO Cells (GPCR (mouse BLT1) stable expression)
with anti-FLAG antibody ( 0.3 μg/ml , 0.14 μg/ml, 0.04 μg/ml, 0.03 μg/ml:2H8, M5 ) and anti-Alexa488-mouse IgG

< Immunoprecipitation >

Fig.5 Immunoprecipitation analysis of extracts from CHO cells with anti-FLAG antibody
(1μg/ml, 0.5 μg/ml, 0.1 μg/ml : 2H8, M2, M5 )

Character

Type Monoclonal Antibody
Immunogen DYKDDDDK peptide expressing cell line
Raised in Mouse (BALB/c)
Source Serum-free medium
Purification ProteinG purified
Cross Reactivity Human
Concentration 0.25 mg/mL
Contents(Volume) 50μg (200μL/vial)
Label Unlabeled
Buffer PBS [containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage Store below 20℃. Once thawed, store at 4℃. Repeated freeze-thaw cycles should be avoided.

Product List

Product Name Cat# Quantity Price

Anti DYKDDDDK

KAL-KO602-S 5UG

¥ 7,600
$ 102
€ 76

Anti DYKDDDDK

KAL-KO602-M 200UG

¥ 30,000
$ 400
€ 300

Anti DYKDDDDK

KAL-KO602-L 1MG

¥ 55,000
$ 734
€ 550

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.