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Anti Human CD44 ICD (intracellular domain) Polyclonal Antibody

Application

Recommended Antibody Dilutions
ELISA WB IHC ICC
Not Tested 15-20 100-150 100-150
IP FCM IF Neutralization
15-20 Not Tested 100-150 Not Tested

(µg/mL)


The data is provided from Division of Gene Regulation Institute for Advanced Medical Research School of Medicine, Keio Univ., Japan

<UniPlot Summary>
# Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
# Subcellular location Membrane ; Single-pass type I membrane protein.
# Tissue specificity Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
# /Sequence similarities Contains 1 Link domain.

Character

Type Polyclonal Antibody
Immunogen Partial peptide of Human CD44 (DQFMTADETRNLQNVDMKIGV)
Raised in Rabbit
Source Rabbit Serum
Purification Protein G purified
Cross Reactivity Human
Concentration 0.25 mg/mL
Contents (Volume) 200µg (800µL/vial)
Label Unlabeled
Buffer PBS [containing2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat]
Storage Store below 20°C. Once thawed, store at 4°C. Repeated freeze-thaw cycles should be avoided.

Product List

Product Name Cat# Quantity Price

Anti CD44ICD

KAL-KO601 200UG

¥ 49,000
$ 654
€ 490

Citation
  • Rheostatic CD44 isoform expression and its association with oxidative stress in human malignant mesothelioma
    Chew SH, Okazaki Y, Akatsuka S, Wang S, Jiang L, Ohara Y, Ito F, Saya H, Sekido Y, Toyokuni S.
    Free Radic Biol Med. 2017 May;106:91-99. PMID:
  • Extravillous trophoblast cell invasion is promoted by the CD44?hyaluronic acid interaction
    Takahashi H, Takizawa T, Matsubara S, Ohkuchi A, Kuwata T, Usui R, Matsumoto H, Sato Y, Fujiwara H, Okamoto A, Suzuki M, Takizawa T.
    Placenta. 2014 Mar;35(3):163-70. PMID: 24439029

References
  • Tumor necrosis factor-alpha regulates transforming growth factor-beta-dependent epithelial-mesenchymal transition by promoting hyaluronan-CD44-moesin interaction.
    Takahashi E, Nagano O, Ishimoto T, Yae T, Suzuki Y, Shinoda T, Nakamura S, Niwa S, Ikeda S, Koga H, Tanihara H, Saya H.
    J Biol Chem. 2010 Feb 5;285(6):4060-73. PMID: 19965872
  • Cell-matrix interaction via CD44 is independently regulated by different metalloproteinases activated in response to extracellular Ca(2+) influx and PKC activation.
    Nagano O, Murakami D, Hartmann D, De Strooper B, Saftig P, Iwatsubo T, Nakajima M, Shinohara M, Saya H.
    J Cell Biol. 2004 Jun 21;165(6):893-902. PMID: 15197174
  • Presenilin-dependent gamma-secretase activity mediates the intramembranous cleavage of CD44.
    Murakami D, Okamoto I, Nagano O, Kawano Y, Tomita T, Iwatsubo T, De Strooper B, Yumoto E, Saya H.
    Oncogene. 2003 Mar 13;22(10):1511-6. PMID: 12629514
  • Proteolytic release of CD44 intracellular domain and its role in the CD44 signaling pathway.
    Okamoto I, Kawano Y, Murakami D, Sasayama T, Araki N, Miki T, Wong AJ, Saya H.
    J Cell Biol. 2001 Nov 26;155(5):755-62. PMID: 11714729
  • Ras oncoprotein induces CD44 cleavage through phosphoinositide 3-OH kinase and the rho family of small G proteins.
    Kawano Y, Okamoto I, Murakami D, Itoh H, Yoshida M, Ueda S, Saya H.
    J Biol Chem. 2000 Sep 22;275(38):29628-35. PMID: 10896935
  • Regulated CD44 cleavage under the control of protein kinase C, calcium influx, and the Rho family of small G proteins.
    Okamoto I, Kawano Y, Matsumoto M, Suga M, Kaibuchi K, Ando M, Saya H.
    J Biol Chem. 1999 Sep 3;274(36):25525-34. PMID: 10464284
  • CD44 cleavage induced by a membrane-associated metalloprotease plays a critical role in tumor cell migration.
    Okamoto I, Kawano Y, Tsuiki H, Sasaki J, Nakao M, Matsumoto M, Suga M, Ando M, Nakajima M, Saya H.
    Oncogene. 1999 Feb 18;18(7):1435-46. PMID: 10050880

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.