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Anti Mouse Sema4b Monoclonal Antibody

Application

WB IHC-P IHC-F IC
1.0 Not Tested Not Tested Not Tested
IP FCM IF Neutralization
5.0 0.05 Not Tested Not Tested

(μg/mL)


[FCM] COS cell expressing mouse Sema4b
The data is provided from Research Institute for Microbial Diseases, Osaka Univ., Japan.

 

<UniPlot Summary>
#  Function // Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
#  Subunit structure // Interacts with GIPC PDZ domain.
#  Subcellular location // Membrane; Single-pass type I membrane protein.
#  Developmental stage // Expressed from day 10 in the embryo. Low levels found between days 10-12. Expression peaks on day 13 with moderate levels from then until birth.
#  Sequence similarities // Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain.

Character

Type Monoclonal Antibody
Immunogen recombinant protein of mouse Sema4B extracellular domain
Raised in Rat
Source Serum-free medium
Purification KAPTIV-M (Columns for Affinity Purification of IgM, Maker: Tecnogen S.p.A.)
Cross Reactivity Mouse
Concentration 0.25 mg/mL
Contents(Volume) 25 ug(100 UL / vial)
Label Unlabeled
Buffer PBS [containing 2% Block Ace as a stabilizer,0.1% Proclin as a bacteriostat]
Storage Store at -20℃ long term, store at 4℃ short term.Avoid repeated freeze-thaw cycles.

<Reference>
1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAAhomologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., et al. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Tissue: Pancreatic islet.
2. "Murine semaphorin D/collapsin is a member of a diverse gene family and creates domains inhibitory for axonal extension." Pueschel A.W., et al. Neuron 14:941-948(1995) [PubMed: 7748561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-823. Strain: NMRI. Tissue: Brain.
3. "A PDZ protein regulates the distribution of the transmembrane semaphorin, M-SemF." Wang L.-H., et al. J. Biol. Chem. 274:14137- 14146(1999) [PubMed: 10318831] [Abstract]

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