Antibiotics have been widely used to reduce the bacterial infection, and continual administration of the antibiotics show increased survival of the bacteria. This phenomenon is of potential medical significance since tolerance causes failures and facilitates the development of antibiotic resistance. The antibiotics have also been used in stockbreeding and fisheries. The antibiotics remained in the meat and fish are ingested by human, and accelerate the development of antibiotic resistance. Among various antibiotics, new quinolones have broad antibiotic spectra and a wide variety of derivatives have been developed and used. Contamination of new quinolones offer problem. We developed monoclonal antibody for the wide variety of new quinolones, and a kit for simultaneous detection of various antibiotics remained in the meat and fish with simple and easy procedure.
|・Assay range : 0.41-300 ng/mL|
|・40 samples can be measured with duplicate|
|・Microtiter plate is divisible strip, 96wells (12 strips, 8 wells)|
|・Sampling volume is 50μL|
|・Total reaction time is 1 hrs &10 minutes.|
|・Store at 2-8℃.The kit is stable for 6 month after the date of manufacture.|
< Specificity >
The ELISA kit is specific for new quinolones and their derivatives, and cross reacts with neither sulfa drugs nor nitrofurans (see Basic Capability).
< Assay principle >
The ELISA kit adopts direct competitive reaction recognizing the basic component in common with new quinolones and their derivatives. The capture antibody are coated on the surface of 96 well plate, onto which HRP-labeled new quinolone, anti-new quinolone antibody and Enrofloxacin antibacterial agent or object to be measured are overlaid at once. Measuring HRP activity in the complex yields concentration of new quinolone in the object to be measured.
|A. Antigen coated 96well plate||96well plate||1 plate||Capture antibody|
|B. New quinolone standard||B0-B7 at eachConcentration per vial||8 vials||50% Methanol containing Enrofloxacin|
|C. Enzyme conjugate solution||60 uL||1 vial||HRP-labeled Newquinolone|
|D. Enzyme conjugatesolution diluent||6 mL||1 vial||Phosphate buffered saline|
|E. Antibody solution||6 mL||1 vial||Phosphate buffered saline containing Anti Newquinolone Antibody|
|F. Substrate for enzymes||12 mL||1 vial||3,3',5,5'-tetramethylbe nzidine (TMB)|
|G. Stop solution||6 mL||1 vial||1N H2SO4|
|H. Concentrated washing solution||50 mL||1 vial||phosphate buffered saline containing Tween20|
|J. Plate seal||1 sheet|
BroadCheck New Quinolone kit Ver.2.0
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.