Reacts with NP of all Influenza B viruses so far tested (113 clinical strains), including Yamagata lineage strains; Mie/1/1993, JohanesBurg/5/1999, Florida/4/2006 and Victoria lineage strains; Lee/1940, Gif/21/1973, Shangdong/7/1997, Malasia/2506/2004, Massachustts/2/2012 No cross reactivity with influenza A viruses.
Example of Immunofluorescence assay, BAM-65-170-EX
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus NP antibody (clone 8C8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus NP antibody (clone 8C8) efficiently detected the viruses in the infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control. The cells infected with an Influenza B virus vaccine strain, Malaysia/2506/2004 as a representative of Victoria group is shown in the upper panel and Florida/4/2006 as Yamagata group in the lower panel.