Please read this entire manual to fully understand and safely use the CBJ-IMR-201-EX.
The Warning sign highlights an instruction that must be strictly followed in order to avoid personal injury. Do not to proceed until the instructions are clearly understood and all statedconditions are met.
Declaration of Conformity and Safety Standards
This product complies with the requirements of Low Voltage
Directive (LVD) 73/23/EEC through the harmonized standard EN 61010-1, 1993+ A1,1992.
This product complies with the requirements of EMC Directive 89/336/EECthrough the harmonized standards EN 50081-1 (emission) and EN 50082-1 (immunity).
The CE symbol and corresponding declaration of conformity are valid forthe instrument when any of the following apply:
Terms and Conditions of Sale
All goods and services are sold subject to the terms and conditions of saleof the company within the Cosmo Bio Co., Ltd., group that supplies them.A copy of these terms and conditions is available on request.
Should you have any comments regarding this product, we will be pleased to receive them at the following address:
COSMO BIO CO.,LTD.
Toyo-Ekimae Bldg., 2-20, Toyo 2-Chome, Kotoku,Tokyo, Japan 135-0016
|Power Supply for i-MyRun||CBJ-IMR-201-EX|
|Electrophorsis Tank for i-MyRun N||CBJ-CBJ-IMR-301-EX-EX|
|Gel Casting System for i-MyRun N||CBJ-IMR-401-EX|
|Support guide for Minigel (L&S)||CBJ-IMR-501-EX|
*For more information refer to "4.Operation"
i-MyRun.N is a novel and innovative electrophoresis system including powersupply, gel electrophoresis tank, and gel casting system.
The power supply (CBJ-IMR-201-EX) is a high-quality, high-precision, andsafe power supply for electrophoresis applications that require a maximumof 135 volts, 80 mA, and a timer is included.
Electrophoresis separations can be controlled by voltage and current. TheCBJ-IMR-201-EX automatically switches over the control parameter accordingto the programmed limits and conductivity variations in the system. Twoelectrophoresis units can be connected to the CBJ-IMR-201-EX and run simultaneously with the same programmed method. One program can be saved (last setting).
Gel Electrophoresis Tank:
The tank lid is equipped with slits to allow electrophoretic patterns tobe easily observed and to optimize heat dissipation. The tank and powersupply are easily set up using one-touch connectors. It is available torun the 96-well plate format, a maximum 156 samples, or the maximum run length of 18.5 mm.
Gel Casting System:
The gel casting system (gel size: 124 (W) x 120 (L) mm) includes a unique UV transparent tray (greater UV transparency for higher sensitivity), a sample loading guide for multichannel handling of 6 combs and 26 wells (volume 9 R). On the other hand, you can use 13 wells (volume of 18 R)if necessary.
2.1 Safety precautions
Extreme caution should be exercised in the operation of this instrument as it can develop sufficient voltage and current to produce a lethal shock.
To avoid any risk of injury, the instrument should only be operated by properly trained personnel and always in accordance with the instructions provided.
Read this entire manual before using this power supply.
2.2 Built-in safety features
The CBJ-IMR-201-EX has been tested and complies with the IEC 61010-1 (EN 61010-1) electrical safety standard.
Use only undamaged electrical wire and equipment approved for the voltage you will use.
Two electrophoresis units can be run simultaneously with the same program. Please remember to double the limiting current and power if two electrophoresis units are run at the same time. The voltage will be the same regardless of the number of units.
1. Set up for the system
The power supply and electrophoresis tank have some of safety interlock systems. If you work with the system, make sure of the following points.
If you remove the lid, the safety function will activate, and you will hearthe beep.
Also make sure of some of the connections.
2. Gel Casting System
This set will produce one large gel.
Dimensions: 124 (W) x 28 (H) x 120 (L) mm
Combs can be positioned at A or B (A1 to A6 or B1 to B4)
Combs allow you to make slots of two different sizes: 18 R(13 wells) or 9 R(26 wells).
Note:The Gel Casting System has a sample loading guide.
1.Make the gels by using the 26-tooth comb on six lanes (A1A6)
|Insert the comb into the hole to which the B mark to the left of the gel casting tray is attached if you need the same four-lane distance.||Insert the comb into the hole to which the A mark to the right of the gel casting tray is attached if you need the same six-lane distance.|
If you with to store gels you have prepared. Be sure to cover the gel with buffer. Store the gel at a low temperature
It is important to keep the gel unit clean, particularly the gel-castingplate, and the combs. Do not clean any part of the gel unit with organic solvents such as ethanol or acetone.
3. Power Supply Operation
Check the Quick Start Operation Manual.
Note:The i-MyRun.N system has the mini gel support guide.
If you want to run your gel at constant voltage:
If you want to run at a constant ampere, apply the same procedures usingthe key instead of (20, 30, 40, 50, 60, 70, or 80 mA can be selected).
This power supply keeps in memory the last voltage and/or current setting in memory.
If you do not wish to use the timer:
You want to stop the electrophoresis:
Select and hold the key for two seconds, OFF appears on the display.
To start select the key.
If the timer is selected, it will automatically restart at the value before the electrophoresis was stopped.
Do not forget to switch off the power supply when not in operation.
ERROR XX is displayed, and the power supply will stop when the current reachesthe limit values.
Code 00: Current is too low.
Code 01: Current is too high.
4. Sample Loading
Gently load the samples into the well using a micropipette. Add small volumes of glycerol or sucrose to the sample facilitate sample. The maximum loading volume of the f sample is about 18 Rfor the 13 wells comb and 9 Rfor the 26 wells comb for the thick gel. If you use a multichannel pipette, use the loading guide plate and follow one of the proposed patterns:
Note: Refer to "4.Operation".
Place the cover on the electrophoresis tank. This will close the electrical circuit.
If the buffer temperature rises above 40 °C during electrophoresis, turn off the power and either dilute the running buffer or place i-MyRun in a cold room. Be aware that if the buffer concentration is too high, the temperature will increase rapidly.
If you want to withdraw the agarose gel from the support, first cut the two small overhangs of agarose located at the end of the gel.
6. Electrical Specification
Voltage from 100 to 240 VA, AC 50/60 Hz
Temperature of operation: from 040 °C
Output constant voltage: 35, 50, 75, 100, 135
VDA Max 54 W or 400 mA
Output constant ampere: 20, 30, 40, 50, 60, 70, 80 mA max 24 W or 300 VG
Wipe the instrument regularly with a damp cloth. Let the instrument drycompletely before use.
Otherwise, no user maintenance is necessary. All servicing should be entrustedto qualified personnel only. Please contact Cosmo Bio Co., Ltd., for more service information.