IMMUNO SHOT -Platinum- is, like IMMUNO SHOT, an enhancer of antigen-antibody reaction. In Western blotting and ELISA, researchers often experiences weak signal or high background. IMMUNO SHOT -Platinum- improves these problems by just using it as antibody diluents. Due to the principle of working mechanism, IMMUNO SHOT -Platinum- can be used in many assay systems that use antigen-antibody reaction.
IMMUNO SHOT -Platinum- is, unlike IMMUNO SHOT, consisted of all chemical components and contains no proteins. So this product is useful for assay systems that have to be avoided protein contamination.
How IMMUNO SHOT -Platinum- Works
IMMUNO SHOT -Platinum- contains polymers. One polymer, by changing the physicochemical properties of antigen and antibody, enhances the mutual accessibility, and facilitate the specific reaction. The other polymer reduces non-specific binding of antibody. Thus, IMMUNO SHOT -Platinum- enhances the antigen-antibody reaction while reducing background
1. High signal with low background
IMMUNO SHOT -Platinum- enhances the antigen-antibody reaction. Comparing with the method using detergent-containing buffer, several to over 10-fold stronger signal can be obtained while the background level is low. Thus, you can get much higher S/N ratio than usual method.
2. Effective for saving antibody usage and time of reaction time
Because higher signal can be obtained using IMMUNO SHOT -Platinum-, you can reduce the amount of antibody used and the time required for reactions.
3. Can be used for many reactions
IMMUNO SHOT -Platinum- can be used not only for Western blotting and ELISA, but also for other assay systems using antigen-antibody reactions. In addition, IMMUNO SHOT -Platinumdoes not affect activities of HRP or ALP, and can be used for assay systems using these enzymes.
4. Easy to use
IMMUNO SHOT -Platinum- is one solution format and formulated as to Ready to Use. Just exchange your dilution buffer of antibodies to IMMUNO SHOT -Platinum- solution.
Principle of usage for IMMUNO SHOT -Platinum-
・ IMMUNO SHOT -Platinum- is one solution format. Just use the same solution for the dilution of the 1st and 2nd antibody. No change is required for the other assay protocol. For details see the later instructions.
・ Do not dilute the IMMUNO SHOT -Platinum- solution. The solution is optimized. Dilution causes loss of IMMUNO SHOT -Platinum-’s ability.
・ IMMUNO SHOT -Platinum- can be used in Western blotting, antibody sandwich ELISA with either the 1st or 2nd antibody-labeled type, antigen sandwich ELISA.
Example 1 Detection of phospho-EGFR
1. IMMUNO SHOT -Platinum-
Sample: A431 Cell Lysate
Example2 Antibody Detection ELISA
|Capture Antigen: Leichmania antigen
Blocking : Blocking Reagent -Chemically Defined-
Antibody: infected serum
Detection: Anti-human IgG-HRP
Example3 Detection of HMG-1
Blocking: Blocking Reagent -Chemically Defined-
To make 100% protein-free assay system, we recommend Blocking Reagent for WB / ELISA-Chemically Defined.
Recommended to use together with Immunoshot Platinum
A: skim milk, TBS-T
B: skim milk,IMMUNO SHOT(1&2)
C: skim milk,IMMUNO SHOT -Platinum-
D: Blocking Reagent-Chemically Defined、IMMUNO SHOT -Platinum-
(Data source: Kyoto University, Graduate School of Biostudies, Negisi laboratory, Hironori Kato)
IMMUNO SHOT -Platinum-
IMMUNO SHOT -Platinum-
Blocking Reagent for WB -Chemically Defined-
Blocking Reagent for ELISA -Chemically Defined-
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.