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An immuno-reaction enhancing solution for immunostaining

IMMUNO SHOT immunostaining

Background

 IMMUNO SHOT Immunostaining is the enhancer of antigen-antibody reaction designed for immunostaining, and enhances specific signal antigen-antibody while reducing nonspecific background staining. Thus, you can get high S/N ratio. Simply exchange the dilution buffer of antibody to IMMUNO SHOT Immunostaining, then you can experience the observation with high S/N ratio. The product is produced based on the technology developed for IMMUNO SHOT which is an antigen-antibody reaction enhancer designed for Western Blotting and ELISA.

 How IMMUNO SHOT Works?
IMMUNO SHOT Immunostaining contains polymers and proteins. One of these agents by changing the physicochemical properties of antigen and antibody, enhances the mutual accessibility, and facilitate the specific reaction. The other ingredients reduces non-specific binding of antibody. Thus, IMMUNO SHOT Immunostaining enhances the antigen-antibody reaction while reduces background.

Application

1. Frozen section <Protocol>

2. Paraffin section <Protocol>

3. Cultured cells <Protocol>

Feature and Advantages

1. Enhance specific signal while reducing background
  IMMUNO SHOT Immunostaining enhances the specific antigen-antibody reaction, while reducing the non-specific binding . Thus, you can get much higher S/N ratio than usual method.

2. Can be used for many chromogenic reaction
  IMMUNO SHOT Immunostaining does not affect activities of HRP (horse radish peroxidase) or ALP (alkaline phosphatase). IMMUNO SHOT Immunostaining can be also used with signal enhancing systems such as ABC system.

3. Easy to use
  IMMUNO SHOT Immunostaining is formulated as to Ready to Use. Just exchange your antibody dilution buffer to IMMUNO SHOT Immunostaining.
(Caution: the signal enhancing effect of IMMUNO SHOT Immunostaining greatly depends on the nature of antibody and antigen, and you may not always get good results)

Others

 Characteristics of Composition

Solution F:
The solution is designed to maximize the background-reducing activity. Ideal for observing fine structures by using antibodies with high specificity and sensitivity.

Solution M:
The solution shows characteristics of middle of F and S. Ideal for initial try.

Solution S:
The solution is designed to maximize the specific signal enhancing activity. Ideal for observing strong signal by using antibodies with less specificity and sensitivity. This solution has less ability to reduce background.
(Caution: the above description shows general characteristics of the solutions, and the results you get may be different depending on the antibody and antigen you are interested in.)

   Trouble shooting

Example

Staining result of Cdk4 in mouse brain tissue

Staining result of Cdk4 in mouse brain tissue
Immuno shot immunostaining showed stromger staining, and showed equal or superior staining comparing competitor.
1st Ab: anti-Cdk4 (rabbit poly), Fixation: 4% paraformaldehyde in PBS, Blocking: 5% FBS in PBS, Stained by ABC (for rabbit) method.
Gulia of forebrain are stained.

 

 Staining result of dimethyl histone in rat brain tissue

Staining result of dimethyl histone in rat brain tissue
Immuno shot immunostaining showed stromger staining, and showed equal or superior staining comparing competitor.
1st Ab: anti-dimethyl histone (Rabbit IgG mAb), Fixation: 4% paraformaldehyde in PBS, Blocking: 5% FBS in PBS, Stained by ABC (for rabbit) method.

 

Staining result of α-Tubulin in A549 cells

Staining result of α-Tubulin in A549 cells
Immuno shot immunostaining showed stromger staining, and showed equal or superior staining comparing competitor.
1st Ab: anti-α-tublin (rabbit poly), 2nd Ab: anti-rabbit IgG (FITC conjugated), Fixation: 4% paraformaldehyde in PBS, Detergent: 0.1% TritonX-100 in PBS, Blocking: 5% FBS in PBS, Stained by ABC (for rabbit) method.

 

 Staining result of p53 in A431cell

Staining result of p53 in A431cell
Immuno shot immunostaining Immuno shot immunostaining showed stromger staining, and showed equal or superior staining comparing competitor.
1st Ab: anti-p53 (rabbit poly, FITC conjugated), Fixation: 4% paraformaldehyde in PBS, Detergent: 0.1% TritonX-100 in PBS, Blocking: 5% FBS in PBS.

 

 Staining result of vimentine in A549cell

Staining result of vimentine in A549cell
Immuno shot immunostaining showed stromger staining, and showed equal or superior staining comparing competitor.
1st Ab: anti-vimentin (rabbit poly), 2nd Ab: anti-rabbit IgG (FITC conjugated), Fixation: 4% paraformaldehyde in PBS, Detergent: 0.1% TritonX-100 in PBS, Blocking: 5% FBS in PBS, Stained by ABC (for rabbit) method.

Product List

Product Name Cat# Quantity Price

IMMUNO SHOT immunostaining, Fine

CSR-IS-F-20 20ML

¥ 15,000
$ 200
€ 150

IMMUNO SHOT immunostaining, Mild

CSR-IS-M-20 20ML

¥ 15,000
$ 200
€ 150

IMMUNO SHOT immunostaining, Strong

CSR-IS-S-20 20ML

¥ 15,000
$ 200
€ 150

IMMUNO SHOT immunostaining Trial

CSR-IS-SMF-10 1SET

¥ 10,000
$ 134
€ 100

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.